Hughes, Christopher C.

Title(s)Chair of Molecular Biology and Biochemistry, Molecular Biology & Biochemistry
Phone(949) 824-8771, 8143, 3379
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    Collapse Research 
    Collapse Research Activities and Funding
    Modular monitoring of hormone release from human islets
    NIH/NIDDK UC4DK116283Sep 15, 2017 - Aug 31, 2021
    Role: Co-Principal Investigator
    Microphysiological systems to model vascular malformations
    NIH/NHLBI UG3HL141799Sep 1, 2017 - Jul 31, 2019
    Role: Principal Investigator
    Real-time bioluminescent detection of circulating biomarkers
    NIH/NIBIB R21EB022704Aug 15, 2016 - May 31, 2018
    Role: Principal Investigator
    A 3-D biomimetic human islet to model beta cell function in health and disease
    NIH/NIDDK UC4DK104202Sep 20, 2014 - Jun 30, 2019
    Role: Co-Principal Investigator
    (PQD5) Development of in Vitro Vascularized Microtumors for Drug Screening
    NIH/NCI R01CA180122Aug 1, 2013 - Jun 30, 2017
    Role: Principal Investigator
    Training Program in Cardiovascular Applied Research and Entrepreneurship
    NIH/NHLBI T32HL116270Jun 1, 2013 - May 31, 2023
    Role: Principal Investigator
    A 3-D In Vitro Platform of Tumor Metastasis (PQ24)
    NIH/NCI R01CA170879Aug 20, 2012 - Jun 30, 2016
    Role: Co-Principal Investigator
    Encapsulated engineered microtumors: A platform for high throughput drug screenin
    NIH/NCI R21CA154187Jan 1, 2011 - Dec 31, 2013
    Role: Principal Investigator
    Creating a prevascularized thick implantable human tissue
    NIH/NHLBI R21HL104203Jun 1, 2010 - Apr 30, 2013
    Role: Co-Principal Investigator
    Bidirectional Notch Signaling and Angiogenesis
    NIH/NHLBI R01HL086959Jan 19, 2007 - Dec 31, 2010
    Role: Principal Investigator
    Molecular Approaches targeted on blocking angiogenesis
    NIH/NHLBI R01HL060067Apr 1, 1999 - Feb 28, 2014
    Role: Principal Investigator
    Endothelial Cell Regulation of T Cell Activation
    NIH/NIAID R01AI040710Jun 1, 1996 - Apr 30, 2007
    Role: Principal Investigator

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    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Faal T, Phan DTT, Davtyan H, Scarfone VM, Varady E, Blurton-Jones M, Hughes CCW, Inlay MA. Induction of Mesoderm and Neural Crest-Derived Pericytes from Human Pluripotent Stem Cells to Study Blood-Brain Barrier Interactions. Stem Cell Reports. 2019 Jan 23. PMID: 30745035.
      View in: PubMed
    2. Hachey SJ, Hughes CCW. Applications of tumor chip technology. Lab Chip. 2018 09 26; 18(19):2893-2912. PMID: 30156248.
      View in: PubMed
    3. Yan H, Romero-López M, Benitez LI, Di K, Frieboes HB, Hughes CCW, Bota DA, Lowengrub JS. Multiscale modeling of glioblastoma. Transl Cancer Res. 2018 Feb; 7(Suppl 1):S96-S98. PMID: 30211018.
      View in: PubMed
    4. Andrejecsk JW, Hosman AE, Botella LM, Shovlin CL, Arthur HM, Dupuis-Girod S, Buscarini E, Hughes CCW, Lebrin F, Mummery CL, Post MC, Mager JJ. Executive summary of the 12th HHT international scientific conference. Angiogenesis. 2018 Feb; 21(1):169-181. PMID: 29147802.
      View in: PubMed
    5. Yan H, Romero-López M, Benitez LI, Di K, Frieboes HB, Hughes CCW, Bota DA, Lowengrub JS. 3D Mathematical Modeling of Glioblastoma Suggests That Transdifferentiated Vascular Endothelial Cells Mediate Resistance to Current Standard-of-Care Therapy. Cancer Res. 2017 08 01; 77(15):4171-4184. PMID: 28536277.
      View in: PubMed
    6. Phan DT, Bender RHF, Andrejecsk JW, Sobrino A, Hachey SJ, George SC, Hughes CC. Blood-brain barrier-on-a-chip: Microphysiological systems that capture the complexity of the blood-central nervous system interface. Exp Biol Med (Maywood). 2017 11; 242(17):1669-1678. PMID: 28195514.
      View in: PubMed
    7. Phan DTT, Wang X, Craver BM, Sobrino A, Zhao D, Chen JC, Lee LYN, George SC, Lee AP, Hughes CCW. A vascularized and perfused organ-on-a-chip platform for large-scale drug screening applications. Lab Chip. 2017 01 31; 17(3):511-520. PMID: 28092382.
      View in: PubMed
    8. Arulmoli J, Wright HJ, Phan DTT, Sheth U, Que RA, Botten GA, Keating M, Botvinick EL, Pathak MM, Zarembinski TI, Yanni DS, Razorenova OV, Hughes CCW, Flanagan LA. Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering. Acta Biomater. 2016 10 01; 43:122-138. PMID: 27475528.
      View in: PubMed
    9. Ziegler ME, Hatch MM, Wu N, Muawad SA, Hughes CC. mTORC2 mediates CXCL12-induced angiogenesis. Angiogenesis. 2016 07; 19(3):359-71. PMID: 27106789.
      View in: PubMed
    10. Wang X, Phan DTT, Zhao D, George SC, Hughes CCW, Lee AP. An on-chip microfluidic pressure regulator that facilitates reproducible loading of cells and hydrogels into microphysiological system platforms. Lab Chip. 2016 Mar 07; 16(5):868-876. PMID: 26879519; PMCID: PMC4911208 [Available on 02/23/17].
    11. Wang X, Phan DT, Sobrino A, George SC, Hughes CC, Lee AP. Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels. Lab Chip. 2016 Jan 21; 16(2):282-90. PMID: 26616908; PMCID: PMC4869859.
    12. Welch-Reardon KM, Wu N, Hughes CC. A role for partial endothelial-mesenchymal transitions in angiogenesis? Arterioscler Thromb Vasc Biol. 2015 Feb; 35(2):303-8. PMID: 25425619; PMCID: PMC4911209.
    13. White SM, Pittman CR, Hingorani R, Arora R, Esipova TV, Vinogradov SA, Hughes CC, Choi B, George SC. Implanted cell-dense prevascularized tissues develop functional vasculature that supports reoxygenation after thrombosis. Tissue Eng Part A. 2014 Sep; 20(17-18):2316-28. PMID: 24593148; PMCID: PMC4161156.
    14. Ehsan SM, Welch-Reardon KM, Waterman ML, Hughes CC, George SC. A three-dimensional in vitro model of tumor cell intravasation. Integr Biol (Camb). 2014 Jun; 6(6):603-10. PMID: 24763498; PMCID: PMC4046910.
    15. Heylman C, Sobrino A, Shirure VS, Hughes CC, George SC. A strategy for integrating essential three-dimensional microphysiological systems of human organs for realistic anticancer drug screening. Exp Biol Med (Maywood). 2014 Sep; 239(9):1240-54. PMID: 24740872; PMCID: PMC4156538.
    16. Popson SA, Ziegler ME, Chen X, Holderfield MT, Shaaban CI, Fong AH, Welch-Reardon KM, Papkoff J, Hughes CC. Interferon-induced transmembrane protein 1 regulates endothelial lumen formation during angiogenesis. Arterioscler Thromb Vasc Biol. 2014 May; 34(5):1011-9. PMID: 24603679; PMCID: PMC3993998.
    17. Welch-Reardon KM, Ehsan SM, Wang K, Wu N, Newman AC, Romero-Lopez M, Fong AH, George SC, Edwards RA, Hughes CC. Angiogenic sprouting is regulated by endothelial cell expression of Slug. J Cell Sci. 2014 May 01; 127(Pt 9):2017-28. PMID: 24554431; PMCID: PMC4004976.
    18. Hsu YH, Moya ML, Hughes CC, George SC, Lee AP. A microfluidic platform for generating large-scale nearly identical human microphysiological vascularized tissue arrays. Lab Chip. 2013 Aug 07; 13(15):2990-8. PMID: 23723013; PMCID: PMC3734340.
    19. Moya ML, Hsu YH, Lee AP, Hughes CC, George SC. In vitro perfused human capillary networks. Tissue Eng Part C Methods. 2013 Sep; 19(9):730-7. PMID: 23320912; PMCID: PMC3719485.
    20. Newman AC, Chou W, Welch-Reardon KM, Fong AH, Popson SA, Phan DT, Sandoval DR, Nguyen DP, Gershon PD, Hughes CC. Analysis of stromal cell secretomes reveals a critical role for stromal cell-derived hepatocyte growth factor and fibronectin in angiogenesis. Arterioscler Thromb Vasc Biol. 2013 Mar; 33(3):513-22. PMID: 23288153; PMCID: PMC3638018.
    21. Hsu YH, Moya ML, Abiri P, Hughes CC, George SC, Lee AP. Full range physiological mass transport control in 3D tissue cultures. Lab Chip. 2013 Jan 07; 13(1):81-9. PMID: 23090158; PMCID: PMC3510322.
    22. Newman AC, Hughes CC. Macrophages and angiogenesis: a role for Wnt signaling. Vasc Cell. 2012 Aug 31; 4(1):13. PMID: 22938389; PMCID: PMC3479425.
    23. Kim JH, Peacock MR, George SC, Hughes CC. BMP9 induces EphrinB2 expression in endothelial cells through an Alk1-BMPRII/ActRII-ID1/ID3-dependent pathway: implications for hereditary hemorrhagic telangiectasia type II. Angiogenesis. 2012 Sep; 15(3):497-509. PMID: 22622516; PMCID: PMC3423339.
    24. White SM, Hingorani R, Arora RP, Hughes CC, George SC, Choi B. Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues. Tissue Eng Part C Methods. 2012 Sep; 18(9):697-709. PMID: 22435776; PMCID: PMC3427639.
    25. Newman AC, Nakatsu MN, Chou W, Gershon PD, Hughes CC. The requirement for fibroblasts in angiogenesis: fibroblast-derived matrix proteins are essential for endothelial cell lumen formation. Mol Biol Cell. 2011 Oct; 22(20):3791-800. PMID: 21865599; PMCID: PMC3192859.
    26. Chen X, Aledia AS, Popson SA, Him L, Hughes CC, George SC. Rapid anastomosis of endothelial progenitor cell-derived vessels with host vasculature is promoted by a high density of cotransplanted fibroblasts. Tissue Eng Part A. 2010 Feb; 16(2):585-94. PMID: 19737050; PMCID: PMC2813071.
    27. Crampton SP, Wu B, Park EJ, Kim JH, Solomon C, Waterman ML, Hughes CC. Integration of the beta-catenin-dependent Wnt pathway with integrin signaling through the adaptor molecule Grb2. PLoS One. 2009 Nov 16; 4(11):e7841. PMID: 19924227; PMCID: PMC2773007.
    28. Chen X, Aledia AS, Ghajar CM, Griffith CK, Putnam AJ, Hughes CC, George SC. Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature. Tissue Eng Part A. 2009 Jun; 15(6):1363-71. PMID: 18976155; PMCID: PMC2792096.
    29. Johnston DA, Dong B, Hughes CC. TNF induction of jagged-1 in endothelial cells is NFkappaB-dependent. Gene. 2009 Apr 15; 435(1-2):36-44. PMID: 19393188; PMCID: PMC2674625.
    30. Hughes CC. Endothelial-stromal interactions in angiogenesis. Curr Opin Hematol. 2008 May; 15(3):204-9. PMID: 18391786.
      View in: PubMed
    31. Holderfield MT, Hughes CC. Crosstalk between vascular endothelial growth factor, notch, and transforming growth factor-beta in vascular morphogenesis. Circ Res. 2008 Mar 28; 102(6):637-52. PMID: 18369162.
      View in: PubMed
    32. Sainson RC, Johnston DA, Chu HC, Holderfield MT, Nakatsu MN, Crampton SP, Davis J, Conn E, Hughes CC. TNF primes endothelial cells for angiogenic sprouting by inducing a tip cell phenotype. Blood. 2008 May 15; 111(10):4997-5007. PMID: 18337563; PMCID: PMC2384130.
    33. Nakatsu MN, Hughes CC. An optimized three-dimensional in vitro model for the analysis of angiogenesis. Methods Enzymol. 2008; 443:65-82. PMID: 18772011.
      View in: PubMed
    34. Ghajar CM, Chen X, Harris JW, Suresh V, Hughes CC, Jeon NL, Putnam AJ, George SC. The effect of matrix density on the regulation of 3-D capillary morphogenesis. Biophys J. 2008 Mar 01; 94(5):1930-41. PMID: 17993494; PMCID: PMC2242748.
    35. Nakatsu MN, Davis J, Hughes CC. Optimized fibrin gel bead assay for the study of angiogenesis. J Vis Exp. 2007; (3):186. PMID: 18978935; PMCID: PMC2570172.
    36. Crampton SP, Davis J, Hughes CC. Isolation of human umbilical vein endothelial cells (HUVEC). J Vis Exp. 2007; (3):183. PMID: 18978951; PMCID: PMC2576276.
    37. Hughes CC. Christopher Hughes: an in vitro model for the study of angiogenesis (interview). J Vis Exp. 2007; (3):175. PMID: 18978990; PMCID: PMC2535841.
    38. Wu B, Crampton SP, Hughes CC. Wnt signaling induces matrix metalloproteinase expression and regulates T cell transmigration. Immunity. 2007 Feb; 26(2):227-39. PMID: 17306568; PMCID: PMC1855210.
    39. Ghajar CM, Blevins KS, Hughes CC, George SC, Putnam AJ. Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early matrix metalloproteinase upregulation. Tissue Eng. 2006 Oct; 12(10):2875-88. PMID: 17518656.
      View in: PubMed
    40. Holderfield MT, Henderson Anderson AM, Kokubo H, Chin MT, Johnson RL, Hughes CC. HESR1/CHF2 suppresses VEGFR2 transcription independent of binding to E-boxes. Biochem Biophys Res Commun. 2006 Aug 04; 346(3):637-48. PMID: 16782059.
      View in: PubMed
    41. Mestas J, Crampton SP, Hori T, Hughes CC. Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA. Int Immunol. 2005 Jun; 17(6):737-47. PMID: 15908450.
      View in: PubMed
    42. Sainson RC, Aoto J, Nakatsu MN, Holderfield M, Conn E, Koller E, Hughes CC. Cell-autonomous notch signaling regulates endothelial cell branching and proliferation during vascular tubulogenesis. FASEB J. 2005 Jun; 19(8):1027-9. PMID: 15774577.
      View in: PubMed
    43. Griffith CK, Miller C, Sainson RC, Calvert JW, Jeon NL, Hughes CC, George SC. Diffusion limits of an in vitro thick prevascularized tissue. Tissue Eng. 2005 Jan-Feb; 11(1-2):257-66. PMID: 15738680.
      View in: PubMed
    44. Mestas J, Hughes CC. Of mice and not men: differences between mouse and human immunology. J Immunol. 2004 Mar 01; 172(5):2731-8. PMID: 14978070.
      View in: PubMed
    45. Nakatsu MN, Sainson RC, Pérez-del-Pulgar S, Aoto JN, Aitkenhead M, Taylor KL, Carpenter PM, Hughes CC. VEGF(121) and VEGF(165) regulate blood vessel diameter through vascular endothelial growth factor receptor 2 in an in vitro angiogenesis model. Lab Invest. 2003 Dec; 83(12):1873-85. PMID: 14691306.
      View in: PubMed
    46. Nakatsu MN, Sainson RC, Aoto JN, Taylor KL, Aitkenhead M, Pérez-del-Pulgar S, Carpenter PM, Hughes CC. Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1. Microvasc Res. 2003 Sep; 66(2):102-12. PMID: 12935768.
      View in: PubMed
    47. Taylor KL, Henderson AM, Hughes CC. Notch activation during endothelial cell network formation in vitro targets the basic HLH transcription factor HESR-1 and downregulates VEGFR-2/KDR expression. Microvasc Res. 2002 Nov; 64(3):372-83. PMID: 12453432.
      View in: PubMed
    48. Murphy LL, Hughes CC. Endothelial cells stimulate T cell NFAT nuclear translocation in the presence of cyclosporin A: involvement of the wnt/glycogen synthase kinase-3 beta pathway. J Immunol. 2002 Oct 01; 169(7):3717-25. PMID: 12244165.
      View in: PubMed
    49. Mazanet MM, Hughes CC. B7-H1 is expressed by human endothelial cells and suppresses T cell cytokine synthesis. J Immunol. 2002 Oct 01; 169(7):3581-8. PMID: 12244148.
      View in: PubMed
    50. Aitkenhead M, Wang SJ, Nakatsu MN, Mestas J, Heard C, Hughes CC. Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: induction of ESM-1, (beta)ig-h3, and NrCAM. Microvasc Res. 2002 Mar; 63(2):159-71. PMID: 11866539.
      View in: PubMed