Loading...

Abraham P. Lee

TitleWilliam J. Link Professor & Chair
InstitutionUniversity of California, Irvine
DepartmentBiomedical Engineering
Address3406 Engineering III
Zot 2715
Irvine CA 92697
Phone(949) 824-9691
vCardDownload vCard

    Collapse Research 
    Collapse Research Activities and Funding
    Microfluidic Engineering of Artificial-Antigen Presenting Cells for the Biomanufacturing of Tumor-Specific T-Cells
    NIH/NIBIB R21EB026900May 1, 2018 - Feb 29, 2020
    Role: Principal Investigator
    "LLISA:'Liposome-Linked Immunosorbant Assay'for Detection of HIV Viral Load at Point-of-Care"
    NIH/NIAID R21AI106024Aug 15, 2013 - Jul 31, 2015
    Role: Co-Principal Investigator
    A 3-D In Vitro Platform of Tumor Metastasis (PQ24)
    NIH/NCI R01CA170879Aug 20, 2012 - Jun 30, 2016
    Role: Co-Principal Investigator
    Integrated Microfluidic Platform for Point-of-Care Serodiagnostics
    NIH/NIAID R43AI102288Aug 1, 2012 - Jul 31, 2014
    Role: Co-Principal Investigator
    Microfluidics systems for formulation of contrast agents for molecular imaging
    NIH/NIBIB R03EB006846Feb 1, 2007 - Jan 31, 2009
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Li X, Lee AP. High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis. Methods Cell Biol. 2018; 148:35-50. PMID: 30473073.
      View in: PubMed
    2. Lee DH, Li X, Jiang A, Lee AP. An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood. Biomicrofluidics. 2018 Sep; 12(5):054104. PMID: 30271519.
      View in: PubMed
    3. Li X, Aghaamoo M, Liu S, Lee DH, Lee AP. Lipoplex-Mediated Single-Cell Transfection via Droplet Microfluidics. Small. 2018 Oct; 14(40):e1802055. PMID: 30199137.
      View in: PubMed
    4. Yale AR, Nourse JL, Lee KR, Ahmed SN, Arulmoli J, Jiang AYL, McDonnell LP, Botten GA, Lee AP, Monuki ES, Demetriou M, Flanagan LA. Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells. Stem Cell Reports. 2018 Aug 29. PMID: 30197120.
      View in: PubMed
    5. Wang X, Zhao D, Phan DTT, Liu J, Chen X, Yang B, Hughes CCW, Zhang W, Lee AP. A hydrostatic pressure-driven passive micropump enhanced with siphon-based autofill function. Lab Chip. 2018 07 24; 18(15):2167-2177. PMID: 29931005.
      View in: PubMed
    6. Lee DH, Li X, Ma N, Digman MA, Lee AP. Rapid and label-free identification of single leukemia cells from blood in a high-density microfluidic trapping array by fluorescence lifetime imaging microscopy. Lab Chip. 2018 05 01; 18(9):1349-1358. PMID: 29638231.
      View in: PubMed
    7. Kamalakshakurup G, Lee AP. High-efficiency single cell encapsulation and size selective capture of cells in picoliter droplets based on hydrodynamic micro-vortices. Lab Chip. 2017 12 05; 17(24):4324-4333. PMID: 29138790.
      View in: PubMed
    8. Nivedita N, Garg N, Lee AP, Papautsky I. A high throughput microfluidic platform for size-selective enrichment of cell populations in tissue and blood samples. Analyst. 2017 Jul 10; 142(14):2558-2569. PMID: 28634607.
      View in: PubMed
    9. Li X, Tao Y, Lee DH, Wickramasinghe HK, Lee AP. In situ mRNA isolation from a microfluidic single-cell array using an external AFM nanoprobe. Lab Chip. 2017 05 02; 17(9):1635-1644. PMID: 28401227.
      View in: PubMed
    10. Phan DTT, Wang X, Craver BM, Sobrino A, Zhao D, Chen JC, Lee LYN, George SC, Lee AP, Hughes CCW. A vascularized and perfused organ-on-a-chip platform for large-scale drug screening applications. Lab Chip. 2017 01 31; 17(3):511-520. PMID: 28092382.
      View in: PubMed
    11. Wang X, Phan DTT, George SC, Hughes CCW, Lee AP. 3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels. Methods Mol Biol. 2017; 1612:325-344. PMID: 28634954.
      View in: PubMed
    12. Sobrino A, Phan DT, Datta R, Wang X, Hachey SJ, Romero-López M, Gratton E, Lee AP, George SC, Hughes CC. 3D microtumors in vitro supported by perfused vascular networks. Sci Rep. 2016 08 23; 6:31589. PMID: 27549930.
      View in: PubMed
    13. Jain A, Taghavian O, Vallejo D, Dotsey E, Schwartz D, Bell FG, Greef C, Davies DH, Grudzien J, Lee AP, Felgner PL, Liang L. Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays. Proteomics. 2016 Apr; 16(8):1271-9. PMID: 26842269; PMCID: PMC4973892 [Available on 04/01/17].
    14. Wang X, Phan DTT, Zhao D, George SC, Hughes CCW, Lee AP. An on-chip microfluidic pressure regulator that facilitates reproducible loading of cells and hydrogels into microphysiological system platforms. Lab Chip. 2016 Mar 07; 16(5):868-876. PMID: 26879519; PMCID: PMC4911208 [Available on 02/23/17].
    15. Shih R, Lee AP. Post-Formation Shrinkage and Stabilization of Microfluidic Bubbles in Lipid Solution. Langmuir. 2016 Mar 01; 32(8):1939-46. PMID: 26820229.
      View in: PubMed
    16. Wang X, Phan DT, Sobrino A, George SC, Hughes CC, Lee AP. Engineering anastomosis between living capillary networks and endothelial cell-lined microfluidic channels. Lab Chip. 2016 Jan 21; 16(2):282-90. PMID: 26616908; PMCID: PMC4869859.
    17. Fang WF, Lee AP. LCAT pump optimization for an integrated microfluidic droplet generator. Microfluid Nanofluidics. 2015 May; 18(5-6):1265-1275. PMID: 30057518.
      View in: PubMed
    18. Simon MG, Li Y, Arulmoli J, McDonnell LP, Akil A, Nourse JL, Lee AP, Flanagan LA. Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput. Biomicrofluidics. 2014 Nov; 8(6):064106. PMID: 25553183; PMCID: PMC4240779.
    19. Bardin D, Lee AP. Low-cost experimentation for the study of droplet microfluidics. Lab Chip. 2014 Oct 21; 14(20):3978-86. PMID: 25133595; PMCID: PMC4190178.
    20. Patel MV, Nanayakkara IA, Simon MG, Lee AP. Cavity-induced microstreaming for simultaneous on-chip pumping and size-based separation of cells and particles. Lab Chip. 2014 Oct 07; 14(19):3860-72. PMID: 25124727.
      View in: PubMed
    21. Hsieh AT, Pan PJ, Lee AP. A real-time characterization method to rapidly optimize molecular beacon signal for sensitive nucleic acids analysis. Anal Bioanal Chem. 2014 May; 406(13):3059-67. PMID: 24722874.
      View in: PubMed
    22. Winkler M, Simon MG, Vu T, Gartner TL, Jester JV, Lee AP, Brown DJ. A microfabricated, optically accessible device to study the effects of mechanical cues on collagen fiber organization. Biomed Microdevices. 2014 Apr; 16(2):255-67. PMID: 24390073.
      View in: PubMed
    23. Shih R, Bardin D, Martz TD, Sheeran PS, Dayton PA, Lee AP. Flow-focusing regimes for accelerated production of monodisperse drug-loadable microbubbles toward clinical-scale applications. Lab Chip. 2013 Dec 21; 13(24):4816-26. PMID: 24162868; PMCID: PMC4062572.
    24. Hsu YH, Moya ML, Hughes CC, George SC, Lee AP. A microfluidic platform for generating large-scale nearly identical human microphysiological vascularized tissue arrays. Lab Chip. 2013 Aug 07; 13(15):2990-8. PMID: 23723013; PMCID: PMC3734340.
    25. Yang CG, Xu ZR, Lee AP, Wang JH. A microfluidic concentration-gradient droplet array generator for the production of multi-color nanoparticles. Lab Chip. 2013 Jul 21; 13(14):2815-20. PMID: 23674199.
      View in: PubMed
    26. Okabe Y, Lee AP. LCAT DNA shearing. J Lab Autom. 2014 Apr; 19(2):163-70. PMID: 23850863.
      View in: PubMed
    27. Bardin D, Kendall MR, Dayton PA, Lee AP. Parallel generation of uniform fine droplets at hundreds of kilohertz in a flow-focusing module. Biomicrofluidics. 2013; 7(3):34112. PMID: 24404032; PMCID: PMC3702567.
    28. Hatch AC, Patel A, Beer NR, Lee AP. Passive droplet sorting using viscoelastic flow focusing. Lab Chip. 2013 Apr 07; 13(7):1308-15. PMID: 23380996.
      View in: PubMed
    29. Moya ML, Hsu YH, Lee AP, Hughes CC, George SC. In vitro perfused human capillary networks. Tissue Eng Part C Methods. 2013 Sep; 19(9):730-7. PMID: 23320912; PMCID: PMC3719485.
    30. Hsu YH, Moya ML, Abiri P, Hughes CC, George SC, Lee AP. Full range physiological mass transport control in 3D tissue cultures. Lab Chip. 2013 Jan 07; 13(1):81-9. PMID: 23090158; PMCID: PMC3510322.
    31. Lu J, Barrios CA, Dickson AR, Nourse JL, Lee AP, Flanagan LA. Advancing practical usage of microtechnology: a study of the functional consequences of dielectrophoresis on neural stem cells. Integr Biol (Camb). 2012 Oct; 4(10):1223-36. PMID: 22892587; PMCID: PMC3808872.
    32. Rane TD, Zec HC, Puleo C, Lee AP, Wang TH. Droplet microfluidics for amplification-free genetic detection of single cells. Lab Chip. 2012 Sep 21; 12(18):3341-7. PMID: 22842841; PMCID: PMC3696383.
    33. Lee A, Wirtanen E. Inspiring engineering minds to advance human health: the Henry Samueli School of Engineering's Department of BME. IEEE Pulse. 2012 Jul; 3(4):42-5. PMID: 23008843.
      View in: PubMed
    34. Kendall MR, Bardin D, Shih R, Dayton PA, Lee AP. Scaled-Up Production of Monodisperse, Dual Layer Microbubbles Using Multi-Array Microfluidic Module for Medical Imaging and Drug Delivery. Bubble Sci Eng Technol. 2012 May; 4(1):12-20. PMID: 23049622.
      View in: PubMed
    35. Lin R, Fisher JS, Simon MG, Lee AP. Novel on-demand droplet generation for selective fluid sample extraction. Biomicrofluidics. 2012 Jun; 6(2):24103-2410310. PMID: 22655015; PMCID: PMC3360719.
    36. Prieto JL, Lu J, Nourse JL, Flanagan LA, Lee AP. Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells. Lab Chip. 2012 Jun 21; 12(12):2182-9. PMID: 22460949.
      View in: PubMed
    37. Martz TD, Bardin D, Sheeran PS, Lee AP, Dayton PA. Microfluidic generation of acoustically active nanodroplets. Small. 2012 Jun 25; 8(12):1876-9. PMID: 22467628; PMCID: PMC3658617.
    38. Simon MG, Lin R, Fisher JS, Lee AP. A Laplace pressure based microfluidic trap for passive droplet trapping and controlled release. Biomicrofluidics. 2012 Mar; 6(1):14110-1411013. PMID: 22662095; PMCID: PMC3365347.
    39. Okabe Y, Chen Y, Purohit R, Corn RM, Lee AP. Piezoelectrically driven vertical cavity acoustic transducers for the convective transport and rapid detection of DNA and protein binding to DNA microarrays with SPR imaging--a parametric study. Biosens Bioelectron. 2012 May 15; 35(1):37-43. PMID: 22503031.
      View in: PubMed
    40. Teh SY, Khnouf R, Fan H, Lee AP. Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics. Biomicrofluidics. 2011 Dec; 5(4):44113-4411312. PMID: 22685501; PMCID: PMC3368830.
    41. Patel MV, Tovar AR, Lee AP. Lateral cavity acoustic transducer as an on-chip cell/particle microfluidic switch. Lab Chip. 2012 Jan 07; 12(1):139-45. PMID: 22072298.
      View in: PubMed
    42. Bardin D, Martz TD, Sheeran PS, Shih R, Dayton PA, Lee AP. High-speed, clinical-scale microfluidic generation of stable phase-change droplets for gas embolotherapy. Lab Chip. 2011 Dec 07; 11(23):3990-8. PMID: 22011845; PMCID: PMC3637946.
    43. Martz TD, Sheeran PS, Bardin D, Lee AP, Dayton PA. Precision manufacture of phase-change perfluorocarbon droplets using microfluidics. Ultrasound Med Biol. 2011 Nov; 37(11):1952-7. PMID: 21963036; PMCID: PMC3291019.
    44. Labeed FH, Lu J, Mulhall HJ, Marchenko SA, Hoettges KF, Estrada LC, Lee AP, Hughes MP, Flanagan LA. Biophysical characteristics reveal neural stem cell differentiation potential. PLoS One. 2011; 6(9):e25458. PMID: 21980464; PMCID: PMC3184132.
    45. Hatch AC, Fisher JS, Tovar AR, Hsieh AT, Lin R, Pentoney SL, Yang DL, Lee AP. 1-Million droplet array with wide-field fluorescence imaging for digital PCR. Lab Chip. 2011 Nov 21; 11(22):3838-45. PMID: 21959960.
      View in: PubMed
    46. Hatch AC, Fisher JS, Pentoney SL, Yang DL, Lee AP. Tunable 3D droplet self-assembly for ultra-high-density digital micro-reactor arrays. Lab Chip. 2011 Aug 07; 11(15):2509-17. PMID: 21670837.
      View in: PubMed
    47. Kaya M, Feingold S, Hettiarachchi K, Lee AP, Dayton PA. Acoustic responses of monodisperse lipid-encapsulated microbubble contrast agents produced by flow focusing. Bubble Sci Eng Technol. 2010 Dec; 2(2):33-40. PMID: 21475641.
      View in: PubMed
    48. Hung LH, Teh SY, Jester J, Lee AP. PLGA micro/nanosphere synthesis by droplet microfluidic solvent evaporation and extraction approaches. Lab Chip. 2010 Jul 21; 10(14):1820-5. PMID: 20467687.
      View in: PubMed
    49. Lin G, Lee AP. Microfluidics: an emerging technology for food and health science. Ann N Y Acad Sci. 2010 Mar; 1190:186-92. PMID: 20388152.
      View in: PubMed
    50. Hettiarachchi K, Lee AP. Polymer-lipid microbubbles for biosensing and the formation of porous structures. J Colloid Interface Sci. 2010 Apr 15; 344(2):521-7. PMID: 20163798; PMCID: PMC2949358.
    51. Hettiarachchi K, Zhang S, Feingold S, Lee AP, Dayton PA. Controllable microfluidic synthesis of multiphase drug-carrying lipospheres for site-targeted therapy. Biotechnol Prog. 2009 Jul-Aug; 25(4):938-45. PMID: 19455647; PMCID: PMC2782552.
    52. Hsieh AT, Hori N, Massoudi R, Pan PJ, Sasaki H, Lin YA, Lee AP. Nonviral gene vector formation in monodispersed picolitre incubator for consistent gene delivery. Lab Chip. 2009 Sep 21; 9(18):2638-43. PMID: 19704978.
      View in: PubMed
    53. Huang CP, Lu J, Seon H, Lee AP, Flanagan LA, Kim HY, Putnam AJ, Jeon NL. Engineering microscale cellular niches for three-dimensional multicellular co-cultures. Lab Chip. 2009 Jun 21; 9(12):1740-8. PMID: 19495458; PMCID: PMC3758562.
    54. Wang L, Lu J, Marchenko SA, Monuki ES, Flanagan LA, Lee AP. Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow-through separation of beads and cells. Electrophoresis. 2009 Mar; 30(5):782-91. PMID: 19197906.
      View in: PubMed
    55. Lee AP. Microfluidic cellular and molecular detection for Lab-on-a-Chip applications. Conf Proc IEEE Eng Med Biol Soc. 2009; 2009:4147-9. PMID: 19963610.
      View in: PubMed
    56. Tovar AR, Lee AP. Lateral cavity acoustic transducer. Lab Chip. 2009 Jan 07; 9(1):41-3. PMID: 19209333.
      View in: PubMed
    57. Hung LH, Lin R, Lee AP. Rapid microfabrication of solvent-resistant biocompatible microfluidic devices. Lab Chip. 2008 Jun; 8(6):983-7. PMID: 18497921.
      View in: PubMed
    58. Talu E, Hettiarachchi K, Powell RL, Lee AP, Dayton PA, Longo ML. Maintaining monodispersity in a microbubble population formed by flow-focusing. Langmuir. 2008 Mar 04; 24(5):1745-9. PMID: 18205422; PMCID: PMC2556210.
    59. Teh SY, Lin R, Hung LH, Lee AP. Droplet microfluidics. Lab Chip. 2008 Feb; 8(2):198-220. PMID: 18231657.
      View in: PubMed
    60. Flanagan LA, Lu J, Wang L, Marchenko SA, Jeon NL, Lee AP, Monuki ES. Unique dielectric properties distinguish stem cells and their differentiated progeny. Stem Cells. 2008 Mar; 26(3):656-65. PMID: 18096719.
      View in: PubMed
    61. Talu E, Hettiarachchi K, Zhao S, Powell RL, Lee AP, Longo ML, Dayton PA. Tailoring the size distribution of ultrasound contrast agents: possible method for improving sensitivity in molecular imaging. Mol Imaging. 2007 Nov-Dec; 6(6):384-92. PMID: 18053409; PMCID: PMC2367094.
    62. Wang L, Flanagan LA, Monuki E, Jeon NL, Lee AP. Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry. Lab Chip. 2007 Sep; 7(9):1114-20. PMID: 17713608; PMCID: PMC3437924.
    63. Hettiarachchi K, Talu E, Longo ML, Dayton PA, Lee AP. On-chip generation of microbubbles as a practical technology for manufacturing contrast agents for ultrasonic imaging. Lab Chip. 2007 Apr; 7(4):463-8. PMID: 17389962; PMCID: PMC2253663.
    64. Hanson KL, Nicolau DV, Filipponi L, Wang L, Lee AP, Nicolau DV. Fungi use efficient algorithms for the exploration of microfluidic networks. Small. 2006 Oct; 2(10):1212-20. PMID: 17193591.
      View in: PubMed
    65. Tan YC, Hettiarachchi K, Siu M, Pan YR, Lee AP. Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles. J Am Chem Soc. 2006 May 03; 128(17):5656-8. PMID: 16637631.
      View in: PubMed
    66. Hung LH, Choi KM, Tseng WY, Tan YC, Shea KJ, Lee AP. Alternating droplet generation and controlled dynamic droplet fusion in microfluidic device for CdS nanoparticle synthesis. Lab Chip. 2006 Feb; 6(2):174-8. PMID: 16450024.
      View in: PubMed
    67. Tan YC, Lee AP. Microfluidic separation of satellite droplets as the basis of a monodispersed micron and submicron emulsification system. Lab Chip. 2005 Oct; 5(10):1178-83. PMID: 16175277.
      View in: PubMed
    68. Chung BG, Flanagan LA, Rhee SW, Schwartz PH, Lee AP, Monuki ES, Jeon NL. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip. 2005 Apr; 5(4):401-6. PMID: 15791337.
      View in: PubMed
    69. Tan YC, Fisher JS, Lee AI, Cristini V, Lee AP. Design of microfluidic channel geometries for the control of droplet volume, chemical concentration, and sorting. Lab Chip. 2004 Aug; 4(4):292-8. PMID: 15269794.
      View in: PubMed
    70. Chao WC, Collins J, Wang SW, Li GP, Bachman M, Lee AP. Control of concentration and volume gradients in microfluidic droplet arrays for protein crystallization screening. Conf Proc IEEE Eng Med Biol Soc. 2004; 4:2623-6. PMID: 17270813.
      View in: PubMed
    71. Lee AP. Digital microfluidics for bioassays and drug delivery. Conf Proc IEEE Eng Med Biol Soc. 2004; 7:5392. PMID: 17271562.
      View in: PubMed
    72. Collins J, Lee AP. Microfluidic flow transducer based on the measurement of electrical admittance. Lab Chip. 2004 Feb; 4(1):7-10. PMID: 15007432.
      View in: PubMed