Hertel, Klemens J

Title(s)Professor, Microbiology and Molecular Genetics
Phone(949) 824-2127, 42128, 42129
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    Collapse Research 
    Collapse Research Activities and Funding
    Tracking Gene Expression Dynamics from Transcription to Degradation
    NIH/NIGMS R01GM110244Mar 1, 2015 - Feb 28, 2019
    Role: Principal Investigator
    Coordinated regulation of alternative pre-mRNA processing in colon cancer
    NIH/NCI R01CA177651May 1, 2014 - Apr 30, 2019
    Role: Principal Investigator
    The role of alternative pre-mRNA splicing in breast cancer progression
    NIH/NCI R21CA149548Jun 1, 2010 - May 31, 2013
    Role: Principal Investigator
    Genomic Analysis of Alternative Splice-Site Selection
    NIH/NIGMS R21GM079413Mar 12, 2007 - Feb 28, 2009
    Role: Principal Investigator
    Mechanisms of Enhancer Dependent SpliceSite Activation
    NIH/NIGMS R01GM062287Feb 1, 2001 - Jun 30, 2020
    Role: Principal Investigator

    Collapse Bibliographic 
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Harari N, Sakhneny L, Khalifa-Malka L, Busch A, Hertel KJ, Hebrok M, Landsman L. Pancreatic pericytes originate from the embryonic pancreatic mesenchyme. Dev Biol. 2019 Feb 13. PMID: 30771302.
      View in: PubMed
    2. Zhu Y, Wang X, Forouzmand E, Jeong J, Qiao F, Sowd GA, Engelman AN, Xie X, Hertel KJ, Shi Y. Molecular Mechanisms for CFIm-Mediated Regulation of mRNA Alternative Polyadenylation. Mol Cell. 2018 01 04; 69(1):62-74.e4. PMID: 29276085.
      View in: PubMed
    3. Movassat M, Shenasa H, Hertel KJ. Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay. Methods Mol Biol. 2017; 1648:11-26. PMID: 28766286.
      View in: PubMed
    4. Garibaldi A, Carranza F, Hertel KJ. Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine. Methods Mol Biol. 2017; 1648:169-176. PMID: 28766297.
      View in: PubMed
    5. Aubol BE, Wu G, Keshwani MM, Movassat M, Fattet L, Hertel KJ, Fu XD, Adams JA. Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing. Mol Cell. 2016 07 21; 63(2):218-228. PMID: 27397683.
      View in: PubMed
    6. Movassat M, Crabb TL, Busch A, Yao C, Reynolds DJ, Shi Y, Hertel KJ. Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns. RNA Biol. 2016 07 02; 13(7):646-55. PMID: 27245359; PMCID: PMC4962795 [Available on 05/31/17].
    7. Mueller WF, Larsen LS, Garibaldi A, Hatfield GW, Hertel KJ. The Silent Sway of Splicing by Synonymous Substitutions. J Biol Chem. 2015 Nov 13; 290(46):27700-11. PMID: 26424794; PMCID: PMC4646019 [Available on 11/13/16].
    8. Busch A, Hertel KJ. Splicing predictions reliably classify different types of alternative splicing. RNA. 2015 May; 21(5):813-23. PMID: 25805853; PMCID: PMC4408789.
    9. Hoverter NP, Zeller MD, McQuade MM, Garibaldi A, Busch A, Selwan EM, Hertel KJ, Baldi P, Waterman ML. The TCF C-clamp DNA binding domain expands the Wnt transcriptome via alternative target recognition. Nucleic Acids Res. 2014 Dec 16; 42(22):13615-32. PMID: 25414359; PMCID: PMC4267635.
    10. Morris JP, Greer R, Russ HA, von Figura G, Kim GE, Busch A, Lee J, Hertel KJ, Kim S, McManus M, Hebrok M. Dicer regulates differentiation and viability during mouse pancreatic cancer initiation. PLoS One. 2014; 9(5):e95486. PMID: 24788257; PMCID: PMC4006805.
    11. von Figura G, Fukuda A, Roy N, Liku ME, Morris Iv JP, Kim GE, Russ HA, Firpo MA, Mulvihill SJ, Dawson DW, Ferrer J, Mueller WF, Busch A, Hertel KJ, Hebrok M. The chromatin regulator Brg1 suppresses formation of intraductal papillary mucinous neoplasm and pancreatic ductal adenocarcinoma. Nat Cell Biol. 2014 Mar; 16(3):255-67. PMID: 24561622.
      View in: PubMed
    12. Movassat M, Mueller WF, Hertel KJ. In vitro assay of pre-mRNA splicing in mammalian nuclear extract. Methods Mol Biol. 2014; 1126:151-60. PMID: 24549662; PMCID: PMC4688300.
    13. Webb CH, Hertel KJ. Preparation of splicing competent nuclear extracts. Methods Mol Biol. 2014; 1126:117-21. PMID: 24549659; PMCID: PMC4501576.
    14. Mueller WF, Hertel KJ. Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract. Methods Mol Biol. 2014; 1126:161-8. PMID: 24549663; PMCID: PMC4688299.
    15. Erkelenz S, Mueller WF, Evans MS, Busch A, Schöneweis K, Hertel KJ, Schaal H. Position-dependent splicing activation and repression by SR and hnRNP proteins rely on common mechanisms. RNA. 2013 Jan; 19(1):96-102. PMID: 23175589; PMCID: PMC3527730.
    16. Busch A, Hertel KJ. HEXEvent: a database of Human EXon splicing Events. Nucleic Acids Res. 2013 Jan; 41(Database issue):D118-24. PMID: 23118488; PMCID: PMC3531206.
    17. Busch A, Hertel KJ. Extensive regulation of NAGNAG alternative splicing: new tricks for the spliceosome? Genome Biol. 2012 Feb 22; 13(2):143. PMID: 22356731; PMCID: PMC3334559.
    18. Busch A, Hertel KJ. Evolution of SR protein and hnRNP splicing regulatory factors. Wiley Interdiscip Rev RNA. 2012 Jan-Feb; 3(1):1-12. PMID: 21898828; PMCID: PMC3235224.
    19. Shepard PJ, Choi EA, Busch A, Hertel KJ. Efficient internal exon recognition depends on near equal contributions from the 3' and 5' splice sites. Nucleic Acids Res. 2011 Nov 01; 39(20):8928-37. PMID: 21795381; PMCID: PMC3203598.
    20. Shepard PJ, Choi EA, Lu J, Flanagan LA, Hertel KJ, Shi Y. Complex and dynamic landscape of RNA polyadenylation revealed by PAS-Seq. RNA. 2011 Apr; 17(4):761-72. PMID: 21343387; PMCID: PMC3062186.
    21. Han J, Ding JH, Byeon CW, Kim JH, Hertel KJ, Jeong S, Fu XD. SR proteins induce alternative exon skipping through their activities on the flanking constitutive exons. Mol Cell Biol. 2011 Feb; 31(4):793-802. PMID: 21135118; PMCID: PMC3028638.
    22. Wang E, Mueller WF, Hertel KJ, Cambi F. G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site. J Biol Chem. 2011 Feb 11; 286(6):4059-71. PMID: 21127064; PMCID: PMC3039333.
    23. Crabb TL, Lam BJ, Hertel KJ. Retention of spliceosomal components along ligated exons ensures efficient removal of multiple introns. RNA. 2010 Sep; 16(9):1786-96. PMID: 20610656; PMCID: PMC2924538.
    24. Shepard PJ, Hertel KJ. Embracing the complexity of pre-mRNA splicing. Cell Res. 2010 Aug; 20(8):866-8. PMID: 20603640; PMCID: PMC3912182.
    25. Hicks MJ, Mueller WF, Shepard PJ, Hertel KJ. Competing upstream 5' splice sites enhance the rate of proximal splicing. Mol Cell Biol. 2010 Apr; 30(8):1878-86. PMID: 20123971; PMCID: PMC2849477.
    26. Geib T, Hertel KJ. Restoration of full-length SMN promoted by adenoviral vectors expressing RNA antisense oligonucleotides embedded in U7 snRNAs. PLoS One. 2009 Dec 08; 4(12):e8204. PMID: 19997596; PMCID: PMC2781471.
    27. Hsu SN, Hertel KJ. Spliceosomes walk the line: splicing errors and their impact on cellular function. RNA Biol. 2009 Nov-Dec; 6(5):526-30. PMID: 19829058; PMCID: PMC3912188.
    28. Shepard PJ, Hertel KJ. The SR protein family. Genome Biol. 2009; 10(10):242. PMID: 19857271; PMCID: PMC2784316.
    29. Tannukit S, Crabb TL, Hertel KJ, Wen X, Jans DA, Paine ML. Identification of a novel nuclear localization signal and speckle-targeting sequence of tuftelin-interacting protein 11, a splicing factor involved in spliceosome disassembly. Biochem Biophys Res Commun. 2009 Dec 18; 390(3):1044-50. PMID: 19857462; PMCID: PMC2787706.
    30. Klaue Y, Hertel KJ. Dangerous play--splitting the message may leave you empty handed. Nat Struct Mol Biol. 2009 Sep; 16(9):907-8. PMID: 19739289.
      View in: PubMed
    31. Fox-Walsh KL, Hertel KJ. Splice-site pairing is an intrinsically high fidelity process. Proc Natl Acad Sci U S A. 2009 Feb 10; 106(6):1766-71. PMID: 19179398; PMCID: PMC2644112.
    32. Kotlajich MV, Crabb TL, Hertel KJ. Spliceosome assembly pathways for different types of alternative splicing converge during commitment to splice site pairing in the A complex. Mol Cell Biol. 2009 Feb; 29(4):1072-82. PMID: 19064642; PMCID: PMC2643811.
    33. Kotlajich MV, Hertel KJ. Death by splicing: tumor suppressor RBM5 freezes splice-site pairing. Mol Cell. 2008 Oct 24; 32(2):162-4. PMID: 18951082.
      View in: PubMed
    34. Shepard PJ, Hertel KJ. Conserved RNA secondary structures promote alternative splicing. RNA. 2008 Aug; 14(8):1463-9. PMID: 18579871; PMCID: PMC2491482.
    35. Hertel KJ. Combinatorial control of exon recognition. J Biol Chem. 2008 Jan 18; 283(3):1211-5. PMID: 18024426.
      View in: PubMed
    36. Sciabica KS, Hertel KJ. The splicing regulators Tra and Tra2 are unusually potent activators of pre-mRNA splicing. Nucleic Acids Res. 2006; 34(22):6612-20. PMID: 17135210; PMCID: PMC1747189.
    37. Dou Y, Fox-Walsh KL, Baldi PF, Hertel KJ. Genomic splice-site analysis reveals frequent alternative splicing close to the dominant splice site. RNA. 2006 Dec; 12(12):2047-56. PMID: 17053087; PMCID: PMC1664720.
    38. Hicks MJ, Yang CR, Kotlajich MV, Hertel KJ. Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns. PLoS Biol. 2006 Jun; 4(6):e147. PMID: 16640457; PMCID: PMC1450099.
    39. Hicks MJ, Lam BJ, Hertel KJ. Analyzing mechanisms of alternative pre-mRNA splicing using in vitro splicing assays. Methods. 2005 Dec; 37(4):306-13. PMID: 16314259.
      View in: PubMed
    40. Fox-Walsh KL, Dou Y, Lam BJ, Hung SP, Baldi PF, Hertel KJ. The architecture of pre-mRNAs affects mechanisms of splice-site pairing. Proc Natl Acad Sci U S A. 2005 Nov 08; 102(45):16176-81. PMID: 16260721; PMCID: PMC1283478.
    41. Madocsai C, Lim SR, Geib T, Lam BJ, Hertel KJ. Correction of SMN2 Pre-mRNA splicing by antisense U7 small nuclear RNAs. Mol Ther. 2005 Dec; 12(6):1013-22. PMID: 16226920.
      View in: PubMed
    42. Freund M, Hicks MJ, Konermann C, Otte M, Hertel KJ, Schaal H. Extended base pair complementarity between U1 snRNA and the 5' splice site does not inhibit splicing in higher eukaryotes, but rather increases 5' splice site recognition. Nucleic Acids Res. 2005; 33(16):5112-9. PMID: 16155183; PMCID: PMC1201333.
    43. Ibrahim EC, Schaal TD, Hertel KJ, Reed R, Maniatis T. Serine/arginine-rich protein-dependent suppression of exon skipping by exonic splicing enhancers. Proc Natl Acad Sci U S A. 2005 Apr 05; 102(14):5002-7. PMID: 15753297; PMCID: PMC555999.
    44. Hertel KJ, Graveley BR. RS domains contact the pre-mRNA throughout spliceosome assembly. Trends Biochem Sci. 2005 Mar; 30(3):115-8. PMID: 15752982.
      View in: PubMed
    45. Lim SR, Hertel KJ. Commitment to splice site pairing coincides with A complex formation. Mol Cell. 2004 Aug 13; 15(3):477-83. PMID: 15304226.
      View in: PubMed
    46. Lam BJ, Bakshi A, Ekinci FY, Webb J, Graveley BR, Hertel KJ. Enhancer-dependent 5'-splice site control of fruitless pre-mRNA splicing. J Biol Chem. 2003 Jun 20; 278(25):22740-7. PMID: 12646561; PMCID: PMC2386364.
    47. Lam BJ, Hertel KJ. A general role for splicing enhancers in exon definition. RNA. 2002 Oct; 8(10):1233-41. PMID: 12403462; PMCID: PMC1370333.