Goulding, Celia

TitleProfessor and Vice Chair
InstitutionUniversity of California, Irvine
DepartmentMolecular Biology & Biochemistry
Address2212 Natural Sciences 1
Zot 3900
Irvine CA 92697
Phone(949) 824-0337
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    Collapse Research 
    Collapse Research Activities and Funding
    Molecular mechanisms of antibacterial CDI toxin activation
    NIH/NIGMS R01GM117373Aug 1, 2016 - Jun 30, 2020
    Role: Co-Principal Investigator
    Structure-function analysis of polymorphic CDI toxin-immunity protein complexes a
    NIH/NIGMS U01GM102318Sep 1, 2012 - Aug 31, 2017
    Role: Principal Investigator
    Structural and Functional Analyses of Toxin-Antitoxin Protein Complexes From Bact
    NIH/NIAID R21AI099687Feb 1, 2012 - Jan 31, 2014
    Role: Principal Investigator
    Structural And Biochemical Characterization Of A Novel Mycobacterial Heme Uptake
    NIH/NIAID R01AI081161Jun 15, 2009 - May 31, 2014
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Chao A, Sieminski PJ, Owens CP, Goulding CW. Iron Acquisition in Mycobacterium tuberculosis. Chem Rev. 2018 Nov 26. PMID: 30474981.
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    2. Thakuri B, Graves AB, Chao A, Johansen SL, Goulding CW, Liptak MD. The affinity of MhuD for heme is consistent with a heme degrading function in vivo. Metallomics. 2018 Sep 21. PMID: 30239544.
      View in: PubMed
    3. Michalska K, Nhan DQ, Willett JLE, Stols LM, Eschenfeldt WH, Jones AM, Nguyen JY, Koskiniemi S, Low DA, Goulding CW, Joachimiak A, Hayes CS. Functional plasticity of antibacterial EndoU toxins. Mol Microbiol. 2018 Jun 20. PMID: 29923643.
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    4. Cuthbert BJ, Burley KH, Goulding CW. Introducing the new bacterial branch of the RNase A superfamily. RNA Biol. 2018 Jan 02; 15(1):9-12. PMID: 29099294.
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    5. Kryshtafovych A, Albrecht R, Baslé A, Bule P, Caputo AT, Carvalho AL, Chao KL, Diskin R, Fidelis K, Fontes CMGA, Fredslund F, Gilbert HJ, Goulding CW, Hartmann MD, Hayes CS, Herzberg O, Hill JC, Joachimiak A, Kohring GW, Koning RI, Lo Leggio L, Mangiagalli M, Michalska K, Moult J, Najmudin S, Nardini M, Nardone V, Ndeh D, Nguyen TH, Pintacuda G, Postel S, van Raaij MJ, Roversi P, Shimon A, Singh AK, Sundberg EJ, Tars K, Zitzmann N, Schwede T. Target highlights from the first post-PSI CASP experiment (CASP12, May-August 2016). Proteins. 2018 Mar; 86 Suppl 1:27-50. PMID: 28960539.
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    6. Michalska K, Gucinski GC, Garza-Sánchez F, Johnson PM, Stols LM, Eschenfeldt WH, Babnigg G, Low DA, Goulding CW, Joachimiak A, Hayes CS. Structure of a novel antibacterial toxin that exploits elongation factor Tu to cleave specific transfer RNAs. Nucleic Acids Res. 2017 Sep 29; 45(17):10306-10320. PMID: 28973472.
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    7. Morrissette NS, Goulding CW. From B to A: making an essential cofactor in a human parasite. Biochem J. 2017 08 30; 474(18):3089-3092. PMID: 28860337.
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    8. Burley K, Goulding CW. Protein engineering: Redirecting membrane machinery. Nat Chem Biol. 2017 Aug 18; 13(9):927-928. PMID: 28820876.
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    9. Batot G, Michalska K, Ekberg G, Irimpan EM, Joachimiak G, Jedrzejczak R, Babnigg G, Hayes CS, Joachimiak A, Goulding CW. The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamily. Nucleic Acids Res. 2017 May 19; 45(9):5013-5025. PMID: 28398546.
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    10. Balderas MA, Nguyen CT, Terwilliger A, Keitel WA, Iniguez A, Torres R, Palacios F, Goulding CW, Maresso AW. Progress toward the Development of a NEAT Protein Vaccine for Anthrax Disease. Infect Immun. 2016 Dec; 84(12):3408-3422. PMID: 27647868.
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    11. Costa DL, Namasivayam S, Amaral EP, Arora K, Chao A, Mittereder LR, Maiga M, Boshoff HI, Barry CE, Goulding CW, Andrade BB, Sher A. Pharmacological Inhibition of Host Heme Oxygenase-1 Suppresses Mycobacterium tuberculosis Infection In Vivo by a Mechanism Dependent on T Lymphocytes. MBio. 2016 10 25; 7(5). PMID: 27795400.
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    12. Johnson PM, Beck CM, Morse RP, Garza-Sánchez F, Low DA, Hayes CS, Goulding CW. Unraveling the essential role of CysK in CDI toxin activation. Proc Natl Acad Sci U S A. 2016 08 30; 113(35):9792-7. PMID: 27531961; PMCID: PMC5024621 [Available on 02/28/17].
    13. Johnson PM, Gucinski GC, Garza-Sánchez F, Wong T, Hung LW, Hayes CS, Goulding CW. Functional Diversity of Cytotoxic tRNase/Immunity Protein Complexes from Burkholderia pseudomallei. J Biol Chem. 2016 09 09; 291(37):19387-400. PMID: 27445337; PMCID: PMC5016678 [Available on 09/09/17].
    14. Diaz-Ochoa VE, Lam D, Lee CS, Klaus S, Behnsen J, Liu JZ, Chim N, Nuccio SP, Rathi SG, Mastroianni JR, Edwards RA, Jacobo CM, Cerasi M, Battistoni A, Ouellette AJ, Goulding CW, Chazin WJ, Skaar EP, Raffatellu M. Salmonella Mitigates Oxidative Stress and Thrives in the Inflamed Gut by Evading Calprotectin-Mediated Manganese Sequestration. Cell Host Microbe. 2016 Jun 08; 19(6):814-25. PMID: 27281571; PMCID: PMC4901528 [Available on 06/08/17].
    15. Matsui T, Nambu S, Goulding CW, Takahashi S, Fujii H, Ikeda-Saito M. Unique coupling of mono- and dioxygenase chemistries in a single active site promotes heme degradation. Proc Natl Acad Sci U S A. 2016 Apr 05; 113(14):3779-84. PMID: 27006503; PMCID: PMC4833273 [Available on 10/05/16].
    16. Morse RP, Willett JL, Johnson PM, Zheng J, Credali A, Iniguez A, Nowick JS, Hayes CS, Goulding CW. Diversification of ß-Augmentation Interactions between CDI Toxin/Immunity Proteins. J Mol Biol. 2015 Nov 20; 427(23):3766-84. PMID: 26449640; PMCID: PMC4658667 [Available on 11/20/16].
    17. Willett JL, Ruhe ZC, Goulding CW, Low DA, Hayes CS. Contact-Dependent Growth Inhibition (CDI) and CdiB/CdiA Two-Partner Secretion Proteins. J Mol Biol. 2015 Nov 20; 427(23):3754-65. PMID: 26388411; PMCID: PMC4658273 [Available on 11/20/16].
    18. Chim N, Torres R, Liu Y, Capri J, Batot G, Whitelegge JP, Goulding CW. The Structure and Interactions of Periplasmic Domains of Crucial MmpL Membrane Proteins from Mycobacterium tuberculosis. Chem Biol. 2015 Aug 20; 22(8):1098-107. PMID: 26278184; PMCID: PMC4546533.
    19. Behera RK, Torres R, Tosha T, Bradley JM, Goulding CW, Theil EC. Fe(2+) substrate transport through ferritin protein cage ion channels influences enzyme activity and biomineralization. J Biol Inorg Chem. 2015 Sep; 20(6):957-69. PMID: 26202907; PMCID: PMC4634868.
    20. Tan K, Johnson PM, Stols L, Boubion B, Eschenfeldt W, Babnigg G, Hayes CS, Joachimiak A, Goulding CW. The structure of a contact-dependent growth-inhibition (CDI) immunity protein from Neisseria meningitidis MC58. Acta Crystallogr F Struct Biol Commun. 2015 Jun; 71(Pt 6):702-9. PMID: 26057799; PMCID: PMC4461334.
    21. Graves AB, Morse RP, Chao A, Iniguez A, Goulding CW, Liptak MD. Crystallographic and spectroscopic insights into heme degradation by Mycobacterium tuberculosis MhuD. Inorg Chem. 2014 Jun 16; 53(12):5931-40. PMID: 24901029; PMCID: PMC4060609.
    22. Contreras H, Joens MS, McMath LM, Le VP, Tullius MV, Kimmey JM, Bionghi N, Horwitz MA, Fitzpatrick JA, Goulding CW. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. J Biol Chem. 2014 Jun 27; 289(26):18279-89. PMID: 24855650; PMCID: PMC4140288.
    23. Beck CM, Morse RP, Cunningham DA, Iniguez A, Low DA, Goulding CW, Hayes CS. CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Structure. 2014 May 06; 22(5):707-18. PMID: 24657090; PMCID: PMC4016183.
    24. Torres R, Lan B, Latif Y, Chim N, Goulding CW. Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase. Acta Crystallogr D Biol Crystallogr. 2014 Apr; 70(Pt 4):1074-85. PMID: 24699651; PMCID: PMC3975890.
    25. Contreras H, Chim N, Credali A, Goulding CW. Heme uptake in bacterial pathogens. Curr Opin Chem Biol. 2014 Apr; 19:34-41. PMID: 24780277; PMCID: PMC4007353.
    26. Chim N, Johnson PM, Goulding CW. Insights into redox sensing metalloproteins in Mycobacterium tuberculosis. J Inorg Biochem. 2014 Apr; 133:118-26. PMID: 24314844; PMCID: PMC3959581.
    27. Chim N, Harmston CA, Guzman DJ, Goulding CW. Structural and biochemical characterization of the essential DsbA-like disulfide bond forming protein from Mycobacterium tuberculosis. BMC Struct Biol. 2013 Oct 18; 13:23. PMID: 24134223; PMCID: PMC3853704.
    28. Pham JD, Chim N, Goulding CW, Nowick JS. Structures of oligomers of a peptide from ß-amyloid. J Am Chem Soc. 2013 Aug 21; 135(33):12460-7. PMID: 23927812; PMCID: PMC3787202.
    29. Owens CP, Chim N, Goulding CW. Insights on how the Mycobacterium tuberculosis heme uptake pathway can be used as a drug target. Future Med Chem. 2013 Aug; 5(12):1391-403. PMID: 23919550; PMCID: PMC3838433.
    30. Owens CP, Chim N, Graves AB, Harmston CA, Iniguez A, Contreras H, Liptak MD, Goulding CW. The Mycobacterium tuberculosis secreted protein Rv0203 transfers heme to membrane proteins MmpL3 and MmpL11. J Biol Chem. 2013 Jul 26; 288(30):21714-28. PMID: 23760277; PMCID: PMC3724630.
    31. Matsui T, Nambu S, Ono Y, Goulding CW, Tsumoto K, Ikeda-Saito M. Heme degradation by Staphylococcus aureus IsdG and IsdI liberates formaldehyde rather than carbon monoxide. Biochemistry. 2013 May 07; 52(18):3025-7. PMID: 23600533; PMCID: PMC3672231.
    32. Nambu S, Matsui T, Goulding CW, Takahashi S, Ikeda-Saito M. A new way to degrade heme: the Mycobacterium tuberculosis enzyme MhuD catalyzes heme degradation without generating CO. J Biol Chem. 2013 Apr 05; 288(14):10101-9. PMID: 23420845; PMCID: PMC3617252.
    33. Honsa ES, Owens CP, Goulding CW, Maresso AW. The near-iron transporter (NEAT) domains of the anthrax hemophore IsdX2 require a critical glutamine to extract heme from methemoglobin. J Biol Chem. 2013 Mar 22; 288(12):8479-90. PMID: 23364793; PMCID: PMC3605663.
    34. Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW. Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems. Proc Natl Acad Sci U S A. 2012 Dec 26; 109(52):21480-5. PMID: 23236156; PMCID: PMC3535622.
    35. Ekworomadu MT, Poor CB, Owens CP, Balderas MA, Fabian M, Olson JS, Murphy F, Bakkalbasi E, Balkabasi E, Honsa ES, He C, Goulding CW, Maresso AW. Differential function of lip residues in the mechanism and biology of an anthrax hemophore. PLoS Pathog. 2012; 8(3):e1002559. PMID: 22412371; PMCID: PMC3297588.
    36. Owens CP, Du J, Dawson JH, Goulding CW. Characterization of heme ligation properties of Rv0203, a secreted heme binding protein involved in Mycobacterium tuberculosis heme uptake. Biochemistry. 2012 Feb 21; 51(7):1518-31. PMID: 22283334; PMCID: PMC3299815.
    37. Torres R, Chim N, Sankaran B, Pujol C, Bliska JB, Goulding CW. Structural insights into RipC, a putative citrate lyase ß subunit from a Yersinia pestis virulence operon. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 01; 68(Pt 1):2-7. PMID: 22232161; PMCID: PMC3253824.
    38. Khan IH, Ravindran R, Krishnan VV, Awan IN, Rizvi SK, Saqib MA, Shahzad MI, Tahseen S, Ireton G, Goulding CW, Felgner P, DeRiemer K, Khanum A, Luciw PA. Plasma antibody profiles as diagnostic biomarkers for tuberculosis. Clin Vaccine Immunol. 2011 Dec; 18(12):2148-53. PMID: 21976221; PMCID: PMC3232686.
    39. Torres R, Swift RV, Chim N, Wheatley N, Lan B, Atwood BR, Pujol C, Sankaran B, Bliska JB, Amaro RE, Goulding CW. Biochemical, structural and molecular dynamics analyses of the potential virulence factor RipA from Yersinia pestis. PLoS One. 2011; 6(9):e25084. PMID: 21966419.
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    40. Tullius MV, Harmston CA, Owens CP, Chim N, Morse RP, McMath LM, Iniguez A, Kimmey JM, Sawaya MR, Whitelegge JP, Horwitz MA, Goulding CW. Discovery and characterization of a unique mycobacterial heme acquisition system. Proc Natl Acad Sci U S A. 2011 Mar 22; 108(12):5051-6. PMID: 21383189; PMCID: PMC3064333.
    41. Chim N, Habel JE, Johnston JM, Krieger I, Miallau L, Sankaranarayanan R, Morse RP, Bruning J, Swanson S, Kim H, Kim CY, Li H, Bulloch EM, Payne RJ, Manos-Turvey A, Hung LW, Baker EN, Lott JS, James MN, Terwilliger TC, Eisenberg DS, Sacchettini JC, Goulding CW. The TB Structural Genomics Consortium: a decade of progress. Tuberculosis (Edinb). 2011 Mar; 91(2):155-72. PMID: 21247804; PMCID: PMC3310434.
    42. Chim N, Riley R, The J, Im S, Segelke B, Lekin T, Yu M, Hung LW, Terwilliger T, Whitelegge JP, Goulding CW. An extracellular disulfide bond forming protein (DsbF) from Mycobacterium tuberculosis: structural, biochemical, and gene expression analysis. J Mol Biol. 2010 Mar 12; 396(5):1211-26. PMID: 20060836; PMCID: PMC2883863.
    43. Chim N, Iniguez A, Nguyen TQ, Goulding CW. Unusual diheme conformation of the heme-degrading protein from Mycobacterium tuberculosis. J Mol Biol. 2010 Jan 22; 395(3):595-608. PMID: 19917297; PMCID: PMC2859679.
    44. Fox BG, Goulding C, Malkowski MG, Stewart L, Deacon A. Structural genomics: from genes to structures with valuable materials and many questions in between. Nat Methods. 2008 Feb; 5(2):129-32. PMID: 18235432.
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    45. Khan IH, Ravindran R, Yee J, Ziman M, Lewinsohn DM, Gennaro ML, Flynn JL, Goulding CW, DeRiemer K, Lerche NW, Luciw PA. Profiling antibodies to Mycobacterium tuberculosis by multiplex microbead suspension arrays for serodiagnosis of tuberculosis. Clin Vaccine Immunol. 2008 Mar; 15(3):433-8. PMID: 18077619; PMCID: PMC2268277.
    46. White EL, Southworth K, Ross L, Cooley S, Gill RB, Sosa MI, Manouvakhova A, Rasmussen L, Goulding C, Eisenberg D, Fletcher TM. A novel inhibitor of Mycobacterium tuberculosis pantothenate synthetase. J Biomol Screen. 2007 Feb; 12(1):100-5. PMID: 17175524.
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    47. Goulding CW, Bowers PM, Segelke B, Lekin T, Kim CY, Terwilliger TC, Eisenberg D. The structure and computational analysis of Mycobacterium tuberculosis protein CitE suggest a novel enzymatic function. J Mol Biol. 2007 Jan 12; 365(2):275-83. PMID: 17064730.
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    48. Strong M, Goulding CW. Structural proteomics and computational analysis of a deadly pathogen: combating Mycobacterium tuberculosis from multiple fronts. Methods Biochem Anal. 2006; 49:245-69. PMID: 16929683.
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    49. Goulding CW, Apostol MI, Sawaya MR, Phillips M, Parseghian A, Eisenberg D. Regulation by oligomerization in a mycobacterial folate biosynthetic enzyme. J Mol Biol. 2005 May 27; 349(1):61-72. PMID: 15876368.
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    50. Goulding CW, Apostol MI, Gleiter S, Parseghian A, Bardwell J, Gennaro M, Eisenberg D. Gram-positive DsbE proteins function differently from Gram-negative DsbE homologs. A structure to function analysis of DsbE from Mycobacterium tuberculosis. J Biol Chem. 2004 Jan 30; 279(5):3516-24. PMID: 14597624.
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    51. Hsu T, Hingley-Wilson SM, Chen B, Chen M, Dai AZ, Morin PM, Marks CB, Padiyar J, Goulding C, Gingery M, Eisenberg D, Russell RG, Derrick SC, Collins FM, Morris SL, King CH, Jacobs WR. The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue. Proc Natl Acad Sci U S A. 2003 Oct 14; 100(21):12420-5. PMID: 14557547; PMCID: PMC218773.
    52. Goulding CW, Perry LJ, Anderson D, Sawaya MR, Cascio D, Apostol MI, Chan S, Parseghian A, Wang SS, Wu Y, Cassano V, Gill HS, Eisenberg D. Structural genomics of Mycobacterium tuberculosis: a preliminary report of progress at UCLA. Biophys Chem. 2003 Sep; 105(2-3):361-70. PMID: 14499904.
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    53. Goulding CW, Perry LJ. Protein production in Escherichia coli for structural studies by X-ray crystallography. J Struct Biol. 2003 Apr; 142(1):133-43. PMID: 12718925.
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    54. Goulding CW, Parseghian A, Sawaya MR, Cascio D, Apostol MI, Gennaro ML, Eisenberg D. Crystal structure of a major secreted protein of Mycobacterium tuberculosis-MPT63 at 1.5-A resolution. Protein Sci. 2002 Dec; 11(12):2887-93. PMID: 12441386; PMCID: PMC2373750.
    55. Goulding CW, Sawaya MR, Parseghian A, Lim V, Eisenberg D, Missiakas D. Thiol-disulfide exchange in an immunoglobulin-like fold: structure of the N-terminal domain of DsbD. Biochemistry. 2002 Jun 04; 41(22):6920-7. PMID: 12033924.
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    56. Goulding CW, Apostol M, Anderson DH, Gill HS, Smith CV, Kuo MR, Yang JK, Waldo GS, Suh SW, Chauhan R, Kale A, Bachhawat N, Mande SC, Johnston JM, Lott JS, Baker EN, Arcus VL, Leys D, McLean KJ, Munro AW, Berendzen J, Sharma V, Park MS, Eisenberg D, Sacchettini J, Alber T, Rupp B, Jacobs W, Terwilliger TC. The TB structural genomics consortium: providing a structural foundation for drug discovery. Curr Drug Targets Infect Disord. 2002 Jun; 2(2):121-41. PMID: 12462144.
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