-
An improved synthesis of guanosine TNA phosphoramidite for oligonucleotide synthesis. Nucleosides Nucleotides Nucleic Acids. 2024 Jun 21; 1-12.
Majumdar B, Sarma D, Lee EM, Setterholm NA, Chaput JC. PMID: 38904107.
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PubMed Mentions: Fields:
-
Improved synthesis and polymerase recognition of 7-deaza-7-modified α-l-threofuranosyl guanosine analogs. RSC Adv. 2024 Jun 18; 14(28):19701-19706.
Barpuzary B, Negria S, Chaput JC. PMID: 38903677; PMCID: PMC11188673.
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PubMed Mentions:
-
Structure and Stability of Ago2 MID-Nucleotide Complexes: All-in-One (Drop) His6-SUMO Tag Removal, Nucleotide Binding, and Crystal Growth. Curr Protoc. 2024 Jun; 4(6):e1088.
Lei L, Harp JM, Chaput JC, Wassarman K, Schlegel MK, Manoharan M, Egli M. PMID: 38923271.
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PubMed Mentions: Fields:
Translation:
HumansCells
-
Functionally Enhanced XNA Aptamers Discovered by Parallelized Library Screening. J Am Chem Soc. 2023 11 29; 145(47):25789-25796.
Lozoya-Colinas A, Yu Y, Chaput JC. PMID: 37962593; PMCID: PMC10690791.
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PubMed Mentions:
1 Fields:
Translation:
Cells
-
Parameterizing the Binding Properties of XNA Aptamers Isolated from a Low Stringency Selection. Biochemistry. 2023 11 21; 62(22):3245-3254.
Kundu N, McCloskey CM, Hajjar M, Chaput JC. PMID: 37932217.
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PubMed Mentions: Fields:
Translation:
Cells
-
Increasing the functional density of threose nucleic acid. RSC Chem Biol. 2024 Jan 03; 5(1):41-48.
Majumdar B, Sarma D, Yu Y, Lozoya-Colinas A, Chaput JC. PMID: 38179195; PMCID: PMC10763562.
View in:
PubMed Mentions:
-
Stability and mechanism of threose nucleic acid toward acid-mediated degradation. Nucleic Acids Res. 2023 Oct 13; 51(18):9542-9551.
Lee EM, Setterholm NA, Hajjar M, Barpuzary B, Chaput JC. PMID: 37650628; PMCID: PMC10570051.
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PubMed Mentions:
3 Fields:
-
Shorter Is Better: The α-(l)-Threofuranosyl Nucleic Acid Modification Improves Stability, Potency, Safety, and Ago2 Binding and Mitigates Off-Target Effects of Small Interfering RNAs. J Am Chem Soc. 2023 09 13; 145(36):19691-19706.
Matsuda S, Bala S, Liao JY, Datta D, Mikami A, Woods L, Harp JM, Gilbert JA, Bisbe A, Manoharan RM, Kim M, Theile CS, Guenther DC, Jiang Y, Agarwal S, Maganti R, Schlegel MK, Zlatev I, Charisse K, Rajeev KG, Castoreno A, Maier M, Janas MM, Egli M, Chaput JC, Manoharan M. PMID: 37638886.
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PubMed Mentions:
1 Fields:
Translation:
Animals
-
Highly Parallelized Screening of Functionally Enhanced XNA Aptamers in Uniform Hydrogel Particles. ACS Synth Biol. 2023 07 21; 12(7):2127-2134.
Yik EJ, Medina E, Paegel BM, Chaput JC. PMID: 37410977.
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PubMed Mentions:
1 Fields:
-
Engineering TNA polymerases through iterative cycles of directed evolution. Methods Enzymol. 2023; 691:29-59.
Yik EJ, Maola VA, Chaput JC. PMID: 37914450.
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PubMed Mentions: Fields:
Translation:
Cells
-
Chemical evolution of an autonomous DNAzyme with allele-specific gene silencing activity. Nat Commun. 2023 04 27; 14(1):2413.
Nguyen K, Malik TN, Chaput JC. PMID: 37105964; PMCID: PMC10140269.
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PubMed Mentions:
3 Fields:
Translation:
Cells
-
Amplification-Free COVID-19 Detection by Digital Droplet REVEALR. ACS Synth Biol. 2023 04 21; 12(4):1331-1338.
Yang K, Chaput JC. PMID: 36947677; PMCID: PMC10042143.
View in:
PubMed Mentions:
1 Fields:
Translation:
HumansCellsPHPublic Health
-
Metabolic adjustments in response to ATP spilling by the small DX protein in a Streptomyces strain. Front Cell Dev Biol. 2023; 11:1129009.
Apel C, Levasseur M, Lejeune C, Korch SB, Guérard F, David M, Askora A, Litaudon M, Roussi F, Gakière B, Chaput J, Virolle MJ. PMID: 36968208; PMCID: PMC10030506.
View in:
PubMed Mentions:
1
-
Crystallographic analysis of engineered polymerases synthesizing phosphonomethylthreosyl nucleic acid. Nucleic Acids Res. 2022 09 23; 50(17):9663-9674.
Hajjar M, Chim N, Liu C, Herdewijn P, Chaput JC. PMID: 36124684; PMCID: PMC9508818.
View in:
PubMed Mentions:
2 Fields:
Translation:
Cells
-
Reply to: On gene silencing by the X10-23 DNAzyme. Nat Chem. 2022 08; 14(8):859-861.
Spitale RC, Chaput JC. PMID: 35851838.
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PubMed Mentions:
2 Fields:
-
REVEALR-Based Genotyping of SARS-CoV-2 Variants of Concern in Clinical Samples. J Am Chem Soc. 2022 07 06; 144(26):11685-11692.
Yang K, Schuder DN, Ngor AK, Chaput JC. PMID: 35729726; PMCID: PMC9236216.
View in:
PubMed Mentions:
4 Fields:
Translation:
HumansCells
-
Transliteration of synthetic genetic enzymes. Nucleic Acids Res. 2021 11 18; 49(20):11438-11446.
Wang Y, Liu X, Shehabat M, Chim N, Chaput JC. PMID: 34634814; PMCID: PMC8599711.
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PubMed Mentions:
1 Fields:
-
Evolution of Functionally Enhanced α-l-Threofuranosyl Nucleic Acid Aptamers. ACS Synth Biol. 2021 11 19; 10(11):3190-3199.
McCloskey CM, Li Q, Yik EJ, Chim N, Ngor AK, Medina E, Grubisic I, Co Ting Keh L, Poplin R, Chaput JC. PMID: 34739228.
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PubMed Mentions:
11 Fields:
-
Synthesis and Polymerase Recognition of Threose Nucleic Acid Triphosphates Equipped with Diverse Chemical Functionalities. J Am Chem Soc. 2021 10 27; 143(42):17761-17768.
Li Q, Maola VA, Chim N, Hussain J, Lozoya-Colinas A, Chaput JC. PMID: 34637287.
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PubMed Mentions:
11 Fields:
Translation:
Cells
-
REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV-2. J Am Chem Soc. 2021 06 23; 143(24):8957-8961.
Yang K, Chaput JC. PMID: 34110141.
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PubMed Mentions:
18 Fields:
Translation:
HumansAnimalsCells
-
Functional Comparison of Laboratory-Evolved XNA Polymerases for Synthetic Biology. ACS Synth Biol. 2021 06 18; 10(6):1429-1437.
Medina E, Yik EJ, Herdewijn P, Chaput JC. PMID: 34029459.
View in:
PubMed Mentions:
11 Fields:
Translation:
Cells
-
Following replicative DNA synthesis by time-resolved X-ray crystallography. Nat Commun. 2021 05 11; 12(1):2641.
Chim N, Meza RA, Trinh AM, Yang K, Chaput JC. PMID: 33976175; PMCID: PMC8113479.
View in:
PubMed Mentions:
7 Fields:
Translation:
Cells
-
A biologically stable DNAzyme that efficiently silences gene expression in cells. Nat Chem. 2021 04; 13(4):319-326.
Wang Y, Nguyen K, Spitale RC, Chaput JC. PMID: 33767363.
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PubMed Mentions:
45 Fields:
Translation:
HumansCells
-
Allele-Specific RNA Knockdown with a Biologically Stable and Catalytically Efficient XNAzyme. J Am Chem Soc. 2021 03 31; 143(12):4519-4523.
Nguyen K, Wang Y, England WE, Chaput JC, Spitale RC. PMID: 33750115.
View in:
PubMed Mentions:
11 Fields:
-
Redesigning the Genetic Polymers of Life. Acc Chem Res. 2021 02 16; 54(4):1056-1065.
Chaput JC. PMID: 33533593.
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PubMed Mentions:
11 Fields:
Translation:
Cells
-
Structural interpretation of the effects of threo-nucleotides on nonenzymatic template-directed polymerization. Nucleic Acids Res. 2021 01 25; 49(2):646-656.
Zhang W, Kim SC, Tam CP, Lelyveld VS, Bala S, Chaput JC, Szostak JW. PMID: 33347562; PMCID: PMC7826252.
View in:
PubMed Mentions:
5 Fields:
Translation:
Cells
-
Reading and Writing Digital Information in TNA. ACS Synth Biol. 2020 11 20; 9(11):2936-2942.
Yang K, McCloskey CM, Chaput JC. PMID: 32966745.
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PubMed Mentions:
8 Fields:
Translation:
Humans
-
In Vitro Selection of an ATP-Binding TNA Aptamer. Molecules. 2020 Sep 13; 25(18).
Zhang L, Chaput JC. PMID: 32933142; PMCID: PMC7570665.
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PubMed Mentions:
5 Fields:
Translation:
Cells
-
Engineering polymerases for applications in synthetic biology. Q Rev Biophys. 2020 07 27; 53:e8.
Nikoomanzar A, Chim N, Yik EJ, Chaput JC. PMID: 32715992.
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PubMed Mentions:
17 Fields:
Translation:
HumansAnimalsCells
-
Synthesis and polymerase recognition of a pyrrolocytidine TNA triphosphate. Biopolymers. 2021 Jan; 112(1):e23388.
Mei H, Wang Y, Yik EJ, Chaput JC. PMID: 32615644.
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PubMed Mentions:
4 Fields:
Translation:
Cells
-
Programmed Allelic Mutagenesis of a DNA Polymerase with Single Amino Acid Resolution. ACS Synth Biol. 2020 07 17; 9(7):1873-1881.
Nikoomanzar A, Vallejo D, Yik EJ, Chaput JC. PMID: 32531152.
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PubMed Mentions:
9 Fields:
Translation:
Cells
-
Directed evolution of custom polymerases using droplet microfluidics. Methods Enzymol. 2020; 644:227-253.
Vallejo D, Nikoomanzar A, Chaput JC. PMID: 32943147.
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PubMed Mentions:
2 Fields:
Translation:
Cells
-
Generating Biologically Stable TNA Aptamers that Function with High Affinity and Thermal Stability. J Am Chem Soc. 2020 04 29; 142(17):7721-7724.
Dunn MR, McCloskey CM, Buckley P, Rhea K, Chaput JC. PMID: 32298104.
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PubMed Mentions:
25 Fields:
Translation:
Humans
-
Orthogonal Genetic Systems. Chembiochem. 2020 05 15; 21(10):1408-1411.
Chaput JC, Herdewijn P, Hollenstein M. PMID: 31889390.
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PubMed Mentions:
10 Fields:
Translation:
HumansAnimalsCells
-
Evaluating the Catalytic Potential of a General RNA-Cleaving FANA Enzyme. Chembiochem. 2020 04 01; 21(7):1001-1006.
Wang Y, Vorperian A, Shehabat M, Chaput JC. PMID: 31680396.
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PubMed Mentions:
3 Fields:
Translation:
Cells
-
P(V) Reagents for the Scalable Synthesis of Natural and Modified Nucleoside Triphosphates. J Am Chem Soc. 2019 08 28; 141(34):13286-13289.
Liao JY, Bala S, Ngor AK, Yik EJ, Chaput JC. PMID: 31298849.
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PubMed Mentions:
12 Fields:
-
Crystal structures of a natural DNA polymerase that functions as an XNA reverse transcriptase. Nucleic Acids Res. 2019 07 26; 47(13):6973-6983.
Jackson LN, Chim N, Shi C, Chaput JC. PMID: 31170294; PMCID: PMC6649750.
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PubMed Mentions:
11 Fields:
Translation:
Cells
-
What Is XNA? Angew Chem Int Ed Engl. 2019 08 19; 58(34):11570-11572.
Chaput JC, Herdewijn P. PMID: 31210402.
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PubMed Mentions:
21 Fields:
-
A Novel Small RNA-Cleaving Deoxyribozyme with a Short Binding Arm. Sci Rep. 2019 06 03; 9(1):8224.
Wang Y, Yang J, Yuan X, Cao J, Xu J, Chaput JC, Li Z, Yu H. PMID: 31160698; PMCID: PMC6546695.
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PubMed Mentions:
2 Fields:
Translation:
Cells
-
Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution. ACS Synth Biol. 2019 06 21; 8(6):1430-1440.
Vallejo D, Nikoomanzar A, Paegel BM, Chaput JC. PMID: 31120731; PMCID: PMC6942126.
View in:
PubMed Mentions:
34 Fields:
Translation:
Cells
-
Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning. ACS Synth Biol. 2019 06 21; 8(6):1421-1429.
Nikoomanzar A, Vallejo D, Chaput JC. PMID: 31081325.
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PubMed Mentions:
11 Fields:
Translation:
Cells
-
RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids. ACS Synth Biol. 2019 05 17; 8(5):955-961.
Horning DP, Bala S, Chaput JC, Joyce GF. PMID: 31042360; PMCID: PMC8111818.
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PubMed Mentions:
9 Fields:
Translation:
Cells
-
Ligase-Mediated Threose Nucleic Acid Synthesis on DNA Templates. ACS Synth Biol. 2019 02 15; 8(2):282-286.
McCloskey CM, Liao JY, Bala S, Chaput JC. PMID: 30629885.
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PubMed Mentions:
6 Fields:
-
Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue. Methods Mol Biol. 2019; 1973:27-37.
Mei H, Chaput J. PMID: 31016694.
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PubMed Mentions: Fields:
-
Activation of Innate Immune Responses by a CpG Oligonucleotide Sequence Composed Entirely of Threose Nucleic Acid. Nucleic Acid Ther. 2019 02; 29(1):51-59.
Lange MJ, Burke DH, Chaput JC. PMID: 30526333; PMCID: PMC6939586.
View in:
PubMed Mentions:
6 Fields:
Translation:
HumansCells
-
Evolution of a General RNA-Cleaving FANA Enzyme. Nat Commun. 2018 11 29; 9(1):5067.
Wang Y, Ngor AK, Nikoomanzar A, Chaput JC. PMID: 30498223; PMCID: PMC6265334.
View in:
PubMed Mentions:
28 Fields:
Translation:
Cells
-
Exploring the Role of AUG Triplets in Human Cap-Independent Translation Enhancing Elements. Biochemistry. 2018 11 06; 57(44):6308-6318.
Juba AN, Chaput JC, Wellensiek BP. PMID: 30371061; PMCID: PMC6222554.
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PubMed Mentions:
4 Fields:
Translation:
HumansCells
-
Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway. Elife. 2018 10 19; 7.
Chim N, Jackson LN, Trinh AM, Chaput JC. PMID: 30338759; PMCID: PMC6231770.
View in:
PubMed Mentions:
8 Fields:
Translation:
Cells
-
Bacterial Genome Containing Chimeric DNA-RNA Sequences. J Am Chem Soc. 2018 09 12; 140(36):11464-11473.
Mehta AP, Wang Y, Reed SA, Supekova L, Javahishvili T, Chaput JC, Schultz PG. PMID: 30160955.
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PubMed Mentions:
5 Fields:
Translation:
Cells
-
Synthesis of 2'-Deoxy-α-l-threofuranosyl Nucleoside Triphosphates. J Org Chem. 2018 08 17; 83(16):8840-8850.
Bala S, Liao JY, Zhang L, Tran CN, Chim N, Chaput JC. PMID: 30011988.
View in:
PubMed Mentions:
1 Fields:
Translation:
Cells
-
Visualizing primer extension without enzymes. Elife. 2018 05 31; 7.
Chaput JC. PMID: 29851382; PMCID: PMC5980227.
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PubMed Mentions: Fields:
-
Synthesis and Evolution of a Threose Nucleic Acid Aptamer Bearing 7-Deaza-7-Substituted Guanosine Residues. J Am Chem Soc. 2018 05 02; 140(17):5706-5713.
Mei H, Liao JY, Jimenez RM, Wang Y, Bala S, McCloskey C, Switzer C, Chaput JC. PMID: 29667819.
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PubMed Mentions:
30 Fields:
-
Made in translation. Nat Chem. 2018 Apr; 10(4):379-381.
Chaput JC. PMID: 29568049.
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PubMed Mentions: Fields:
Translation:
Cells
-
Expanding the chemical diversity of TNA with tUTP derivatives that are substrates for a TNA polymerase. Chem Commun (Camb). 2018 Jan 31; 54(10):1237-1240.
Mei H, Chaput JC. PMID: 29340357.
View in:
PubMed Mentions:
3 Fields:
-
A Tool for the Import of Natural and Unnatural Nucleoside Triphosphates into Bacteria. J Am Chem Soc. 2018 01 31; 140(4):1447-1454.
Feldman AW, Fischer EC, Ledbetter MP, Liao JY, Chaput JC, Romesberg FE. PMID: 29338214; PMCID: PMC5809120.
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PubMed Mentions:
14 Fields:
Translation:
AnimalsCells
-
Structural basis for TNA synthesis by an engineered TNA polymerase. Nat Commun. 2017 11 27; 8(1):1810.
Chim N, Shi C, Sau SP, Nikoomanzar A, Chaput JC. PMID: 29180809; PMCID: PMC5703726.
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PubMed Mentions:
17 Fields:
Translation:
Cells
-
Evaluating the Rate and Substrate Specificity of Laboratory Evolved XNA Polymerases. Anal Chem. 2017 12 05; 89(23):12622-12625.
Nikoomanzar A, Dunn MR, Chaput JC. PMID: 29148714.
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PubMed Mentions:
3 Fields:
Translation:
Cells
-
A Gram-Scale HPLC-Free Synthesis of TNA Triphosphates Using an Iterative Phosphorylation Strategy. Org Lett. 2017 08 18; 19(16):4379-4382.
Sau SP, Chaput JC. PMID: 28766949.
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PubMed Mentions:
4 Fields:
-
Engineered Polymerases with Altered Substrate Specificity: Expression and Purification. Curr Protoc Nucleic Acid Chem. 2017 06 19; 69:4.75.1-4.75.20.
Nikoomanzar A, Dunn MR, Chaput JC. PMID: 28628207.
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PubMed Mentions:
7 Fields:
Translation:
Cells
-
Synthesis and polymerase activity of a fluorescent cytidine TNA triphosphate analogue. Nucleic Acids Res. 2017 Jun 02; 45(10):5629-5638.
Mei H, Shi C, Jimenez RM, Wang Y, Kardouh M, Chaput JC. PMID: 28472363; PMCID: PMC5449585.
View in:
PubMed Mentions:
11 Fields:
Translation:
Cells
-
Synthesis of α-l-Threofuranosyl Nucleoside 3'-Monophosphates, 3'-Phosphoro(2-Methyl)imidazolides, and 3'-Triphosphates. J Org Chem. 2017 06 02; 82(11):5910-5916.
Bala S, Liao JY, Mei H, Chaput JC. PMID: 28490177.
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PubMed Mentions:
5 Fields:
-
A parallel stranded G-quadruplex composed of threose nucleic acid (TNA). Biopolymers. 2017 Mar; 107(3).
Liao JY, Anosova I, Bala S, Van Horn WD, Chaput JC. PMID: 27718227.
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PubMed Mentions:
5 Fields:
Translation:
Cells
-
Reverse Transcription of Threose Nucleic Acid by a Naturally Occurring DNA Polymerase. Chembiochem. 2016 10 04; 17(19):1804-1808.
Dunn MR, Chaput JC. PMID: 27383648.
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PubMed Mentions:
20 Fields:
Translation:
HumansCells
-
Structural Insights into Conformation Differences between DNA/TNA and RNA/TNA Chimeric Duplexes. Chembiochem. 2016 09 15; 17(18):1705-8.
Anosova I, Kowal EA, Sisco NJ, Sau S, Liao JY, Bala S, Rozners E, Egli M, Chaput JC, Van Horn WD. PMID: 27347671; PMCID: PMC5242226.
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PubMed Mentions:
12 Fields:
Translation:
Cells
-
A one-pot synthesis of α-l-threofuranosyl nucleoside triphosphates (tNTPs). Bioorg Med Chem Lett. 2016 07 15; 26(14):3271-3273.
Sau SP, Chaput JC. PMID: 27246616.
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PubMed Mentions:
5 Fields:
Translation:
Cells
-
A general strategy for expanding polymerase function by droplet microfluidics. Nat Commun. 2016 Apr 05; 7:11235.
Larsen AC, Dunn MR, Hatch A, Sau SP, Youngbull C, Chaput JC. PMID: 27044725; PMCID: PMC4822039.
View in:
PubMed Mentions:
53 Fields:
Translation:
Cells
-
Evaluating TNA stability under simulated physiological conditions. Bioorg Med Chem Lett. 2016 05 15; 26(10):2418-2421.
Culbertson MC, Temburnikar KW, Sau SP, Liao JY, Bala S, Chaput JC. PMID: 27080186.
View in:
PubMed Mentions:
20 Fields:
Translation:
HumansCells
-
A Scalable Synthesis of α-L-Threose Nucleic Acid Monomers. J Org Chem. 2016 Mar 18; 81(6):2302-7.
Sau SP, Fahmi NE, Liao JY, Bala S, Chaput JC. PMID: 26895480.
View in:
PubMed Mentions:
21 Fields:
-
Improving Polymerase Activity with Unnatural Substrates by Sampling Mutations in Homologous Protein Architectures. ACS Chem Biol. 2016 05 20; 11(5):1210-9.
Dunn MR, Otto C, Fenton KE, Chaput JC. PMID: 26860781.
View in:
PubMed Mentions:
23 Fields:
Translation:
Cells
-
The structural diversity of artificial genetic polymers. Nucleic Acids Res. 2016 Feb 18; 44(3):1007-21.
Anosova I, Kowal EA, Dunn MR, Chaput JC, Van Horn WD, Egli M. PMID: 26673703; PMCID: PMC4756832.
View in:
PubMed Mentions:
57 Fields:
Translation:
Cells
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Comparative analysis of eukaryotic cell-free expression systems. Biotechniques. 2015 Sep; 59(3):149-51.
Hartsough EM, Shah P, Larsen AC, Chaput JC. PMID: 26345507.
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PubMed Mentions:
2 Fields:
Translation:
HumansAnimalsCells
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DNA polymerase-mediated synthesis of unbiased threose nucleic acid (TNA) polymers requires 7-deazaguanine to suppress G:G mispairing during TNA transcription. J Am Chem Soc. 2015 Apr 01; 137(12):4014-7.
Dunn MR, Larsen AC, Zahurancik WJ, Fahmi NE, Meyers M, Suo Z, Chaput JC. PMID: 25785966.
View in:
PubMed Mentions:
7 Fields:
Translation:
Cells
-
Automated solid-phase synthesis of high capacity oligo-dT cellulose for affinity purification of poly-A tagged biomolecules. Bioorg Med Chem Lett. 2014 Dec 15; 24(24):5692-5694.
Sau SP, Larsen AC, Chaput JC. PMID: 25467163.
View in:
PubMed Mentions:
3 Fields:
Translation:
Humans
-
Replicating an expanded genetic alphabet in cells. Chembiochem. 2014 Sep 05; 15(13):1869-71.
Chaput JC. PMID: 25044483.
View in:
PubMed Mentions:
1 Fields:
Translation:
Cells
-
General approach for characterizing in vitro selected peptides with protein binding affinity. Anal Chem. 2014 Aug 05; 86(15):7219-23.
Larsen AC, Gillig A, Shah P, Sau SP, Fenton KE, Chaput JC. PMID: 24970615; PMCID: PMC4215864.
View in:
PubMed Mentions:
4 Fields:
Translation:
Cells
-
An In Vitro Selection Protocol for Threose Nucleic Acid (TNA) Using DNA Display. Curr Protoc Nucleic Acid Chem. 2014 Jun 24; 57:9.8.1-19.
Dunn MR, Chaput JC. PMID: 24961723.
View in:
PubMed Mentions:
1 Fields:
Translation:
Cells
-
Development of a full-length human protein production pipeline. Protein Sci. 2014 Aug; 23(8):1123-35.
Saul J, Petritis B, Sau S, Rauf F, Gaskin M, Ober-Reynolds B, Mineyev I, Magee M, Chaput J, Qiu J, LaBaer J. PMID: 24806540; PMCID: PMC4116660.
View in:
PubMed Mentions:
7 Fields:
Translation:
HumansCells
-
A leader sequence capable of enhancing RNA expression and protein synthesis in mammalian cells. Protein Sci. 2013 Oct; 22(10):1392-8.
Wellensiek BP, Larsen AC, Flores J, Jacobs BL, Chaput JC. PMID: 23908110; PMCID: PMC3795497.
View in:
PubMed Mentions:
6 Fields:
Translation:
HumansAnimalsCells
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Genome-wide profiling of human cap-independent translation-enhancing elements. Nat Methods. 2013 Aug; 10(8):747-50.
Wellensiek BP, Larsen AC, Stephens B, Kukurba K, Waern K, Briones N, Liu L, Snyder M, Jacobs BL, Kumar S, Chaput JC. PMID: 23770754; PMCID: PMC3731418.
View in:
PubMed Mentions:
19 Fields:
Translation:
HumansCells
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Synthesis of threose nucleic acid (TNA) triphosphates and oligonucleotides by polymerase-mediated primer extension. Curr Protoc Nucleic Acid Chem. 2013 Mar; Chapter 4:Unit 4.54.
Zhang S, Yu H, Chaput JC. PMID: 23512696.
View in:
PubMed Mentions:
3 Fields:
-
An efficient and faithful in vitro replication system for threose nucleic acid. J Am Chem Soc. 2013 Mar 06; 135(9):3583-91.
Yu H, Zhang S, Dunn MR, Chaput JC. PMID: 23432469.
View in:
PubMed Mentions:
17 Fields:
Translation:
Cells
-
Synthesis and enzymatic incorporation of α-L-threofuranosyl adenine triphosphate (tATP). Bioorg Med Chem Lett. 2013 Mar 01; 23(5):1447-9.
Zhang S, Chaput JC. PMID: 23352269.
View in:
PubMed Mentions:
1 Fields:
Translation:
Humans
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ATP sequestration by a synthetic ATP-binding protein leads to novel phenotypic changes in Escherichia coli. ACS Chem Biol. 2013 Feb 15; 8(2):451-63.
Korch SB, Stomel JM, León MA, Hamada MA, Stevenson CR, Simpson BW, Gujulla SK, Chaput JC. PMID: 23181457.
View in:
PubMed Mentions:
2 Fields:
Translation:
Cells
-
The emerging world of synthetic genetics. Chem Biol. 2012 Nov 21; 19(11):1360-71.
Chaput JC, Yu H, Zhang S. PMID: 23177191.
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PubMed Mentions:
27 Fields:
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Synthesis of threose nucleic acid (TNA) phosphoramidite monomers and oligonucleotide polymers. Curr Protoc Nucleic Acid Chem. 2012 Sep; Chapter 4:Unit4.51.
Zhang S, Chaput JC. PMID: 22956457.
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PubMed Mentions:
4 Fields:
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Solution structure of a parallel-stranded oligoisoguanine DNA pentaplex formed by d(T(iG)4 T) in the presence of Cs+ ions. Angew Chem Int Ed Engl. 2012 Aug 06; 51(32):7952-5.
Kang M, Heuberger B, Chaput JC, Switzer C, Feigon J. PMID: 22767484.
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PubMed Mentions:
4 Fields:
Translation:
Cells
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Synthetic genetic polymers capable of heredity and evolution. Science. 2012 Apr 20; 336(6079):341-4.
Pinheiro VB, Taylor AI, Cozens C, Abramov M, Renders M, Zhang S, Chaput JC, Wengel J, Peak-Chew SY, McLaughlin SH, Herdewijn P, Holliger P. PMID: 22517858; PMCID: PMC3362463.
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PubMed Mentions:
207 Fields:
Translation:
Cells
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Darwinian evolution of an alternative genetic system provides support for TNA as an RNA progenitor. Nat Chem. 2012 Jan 10; 4(3):183-7.
Yu H, Zhang S, Chaput JC. PMID: 22354431.
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PubMed Mentions:
78 Fields:
Translation:
HumansAnimalsCells
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Aptamers can discriminate alkaline proteins with high specificity. Chembiochem. 2011 Nov 25; 12(17):2659-66.
Yu H, Jiang B, Chaput JC. PMID: 22021204; PMCID: PMC3517100.
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PubMed Mentions:
8 Fields:
Translation:
Cells
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Generating DNA synbodies from previously discovered peptides. Chembiochem. 2011 Aug 16; 12(12):1813-7.
Liu R, Jiang B, Yu H, Chaput JC. PMID: 21692159; PMCID: PMC3390916.
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PubMed Mentions:
2 Fields:
Translation:
HumansCells
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Three-dimensional structures reveal multiple ADP/ATP binding modes for a synthetic class of artificial proteins. Biochemistry. 2010 Oct 12; 49(40):8689-99.
Simmons CR, Magee CL, Smith DA, Lauman L, Chaput JC, Allen JP. PMID: 20822107.
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Translation:
Cells
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The evolvability of lead peptides from small library screens. Chem Commun (Camb). 2010 Nov 07; 46(41):7778-80.
Zhang J, Williams BA, Nilsson MT, Chaput JC. PMID: 20830334.
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PubMed Mentions:
3 Fields:
Translation:
HumansCells
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Synthesis of glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers. Curr Protoc Nucleic Acid Chem. 2010 Sep; Chapter 4:Unit4.40.
Zhang S, Chaput JC. PMID: 20827716.
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PubMed Mentions:
2 Fields:
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Synthesis of peptide-oligonucleotide conjugates using a heterobifunctional crosslinker. Curr Protoc Nucleic Acid Chem. 2010 Sep; Chapter 4:Unit4.41.
Williams BA, Chaput JC. PMID: 20827717; PMCID: PMC2947322.
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PubMed Mentions:
9 Fields:
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The resurgence of acyclic nucleic acids. Chem Biodivers. 2010 Feb; 7(2):245-58.
Zhang S, Switzer C, Chaput JC. PMID: 20151377.
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PubMed Mentions:
7 Fields:
Translation:
Cells
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Random mutagenesis by error-prone PCR. Methods Mol Biol. 2010; 634:103-9.
McCullum EO, Williams BA, Zhang J, Chaput JC. PMID: 20676978.
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PubMed Mentions:
62 Fields:
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Creating protein affinity reagents by combining peptide ligands on synthetic DNA scaffolds. J Am Chem Soc. 2009 Dec 02; 131(47):17233-41.
Williams BA, Diehnelt CW, Belcher P, Greving M, Woodbury NW, Johnston SA, Chaput JC. PMID: 19894711; PMCID: PMC2796340.
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PubMed Mentions:
24 Fields:
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A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells. PLoS One. 2009 Oct 08; 4(10):e7385.
Stomel JM, Wilson JW, León MA, Stafford P, Chaput JC. PMID: 19812699; PMCID: PMC2754611.
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PubMed Mentions:
5 Fields:
Translation:
Cells
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Recognition imaging of acetylated chromatin using a DNA aptamer. Biophys J. 2009 Sep 16; 97(6):1804-7.
Lin L, Fu Q, Williams BA, Azzaz AM, Shogren-Knaak MA, Chaput JC, Lindsay S. PMID: 19751687; PMCID: PMC2749789.
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PubMed Mentions:
5 Fields:
Translation:
AnimalsCells
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Conformational analysis of DNA repair intermediates by time-resolved fluorescence spectroscopy. J Phys Chem A. 2009 Sep 03; 113(35):9585-7.
Lin S, Horning DP, Szostak JW, Chaput JC. PMID: 19673467; PMCID: PMC2733762.
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PubMed Mentions:
7 Fields:
Translation:
Cells
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A synthetic protein selected for ligand binding affinity mediates ATP hydrolysis. ACS Chem Biol. 2009 Aug 21; 4(8):649-58.
Simmons CR, Stomel JM, McConnell MD, Smith DA, Watkins JL, Allen JP, Chaput JC. PMID: 19522480.
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PubMed Mentions:
3 Fields:
Translation:
HumansCells
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Evolution of a histone H4-K16 acetyl-specific DNA aptamer. J Am Chem Soc. 2009 May 13; 131(18):6330-1.
Williams BA, Lin L, Lindsay SM, Chaput JC. PMID: 19385619; PMCID: PMC2715991.
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PubMed Mentions:
16 Fields:
Translation:
HumansCells
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Transcription of an RNA aptamer by a DNA polymerase. Chem Commun (Camb). 2009 May 28; (20):2938-40.
McCullum EO, Chaput JC. PMID: 19436916.
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PubMed Mentions:
2 Fields:
Translation:
Cells
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Template-directed nucleation and growth of inorganic nanoparticles on DNA scaffolds. Angew Chem Int Ed Engl. 2009; 48(45):8494-6.
Stearns LA, Chhabra R, Sharma J, Liu Y, Petuskey WT, Yan H, Chaput JC. PMID: 19795428.
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PubMed Mentions:
18 Fields:
Translation:
Cells
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Creating protein biocatalysts as tools for future industrial applications. Expert Opin Biol Ther. 2008 Aug; 8(8):1087-98.
Chaput JC, Woodbury NW, Stearns LA, Williams BA. PMID: 18613761.
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PubMed Mentions:
4 Fields:
Translation:
Cells
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Searching combinatorial libraries for native proteins with novel folds. Chembiochem. 2008 Jun 16; 9(9):1361-3.
Watkins JL, Chaput JC. PMID: 18464233.
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PubMed Mentions:
1 Fields:
Translation:
Cells
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Synthesis of two mirror image 4-helix junctions derived from glycerol nucleic acid. J Am Chem Soc. 2008 May 07; 130(18):5846-7.
Zhang RS, McCullum EO, Chaput JC. PMID: 18407636.
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PubMed Mentions:
10 Fields:
Translation:
Cells
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In vitro selection of histone H4 aptamers for recognition imaging microscopy. J Am Chem Soc. 2007 Nov 28; 129(47):14568-9.
Lin L, Hom D, Lindsay SM, Chaput JC. PMID: 17985909; PMCID: PMC2533041.
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PubMed Mentions:
8 Fields:
Translation:
Cells
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Experimental evidence that GNA and TNA were not sequential polymers in the prebiotic evolution of RNA. J Mol Evol. 2007 Sep; 65(3):289-95.
Yang YW, Zhang S, McCullum EO, Chaput JC. PMID: 17828568.
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PubMed Mentions:
14 Fields:
Translation:
Cells
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Structure and evolutionary analysis of a non-biological ATP-binding protein. J Mol Biol. 2007 Aug 10; 371(2):501-13.
Mansy SS, Zhang J, Kümmerle R, Nilsson M, Chou JJ, Szostak JW, Chaput JC. PMID: 17583732.
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PubMed Mentions:
6 Fields:
Translation:
Cells
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Structural insights into the evolution of a non-biological protein: importance of surface residues in protein fold optimization. PLoS One. 2007 May 23; 2(5):e467.
Smith MD, Rosenow MA, Wang M, Allen JP, Szostak JW, Chaput JC. PMID: 17520026; PMCID: PMC1867856.
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PubMed Mentions:
4 Fields:
Translation:
Cells
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Self-assembled peptide nanoarrays: an approach to studying protein-protein interactions. Angew Chem Int Ed Engl. 2007; 46(17):3051-4.
Williams BA, Lund K, Liu Y, Yan H, Chaput JC. PMID: 17361972.
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PubMed Mentions:
30 Fields:
Translation:
Cells
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Kinetic analysis of an efficient DNA-dependent TNA polymerase. J Am Chem Soc. 2005 May 25; 127(20):7427-34.
Horhota A, Zou K, Ichida JK, Yu B, McLaughlin LW, Szostak JW, Chaput JC. PMID: 15898792; PMCID: PMC5042361.
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PubMed Mentions:
39 Fields:
Translation:
Cells
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Evolutionary optimization of a nonbiological ATP binding protein for improved folding stability. Chem Biol. 2004 Jun; 11(6):865-74.
Chaput JC, Szostak JW. PMID: 15217619.
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PubMed Mentions:
11 Fields:
Translation:
Cells
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TNA synthesis by DNA polymerases. J Am Chem Soc. 2003 Aug 06; 125(31):9274-5.
Chaput JC, Szostak JW. PMID: 12889939.
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PubMed Mentions:
28 Fields:
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DNA polymerase-mediated DNA synthesis on a TNA template. J Am Chem Soc. 2003 Jan 29; 125(4):856-7.
Chaput JC, Ichida JK, Szostak JW. PMID: 12537469.
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PubMed Mentions:
30 Fields:
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5-propynyluracil.diaminopurine: an efficient base-pair for non-enzymatic transcription of DNA. Chem Commun (Camb). 2002 Aug 07; (15):1568-9.
Chaput JC, Sinha S, Switzer C. PMID: 12170785.
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PubMed Mentions:
8 Fields:
Translation:
Cells
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Probing structure and function with alternative nucleic acids bearing 2',5'-linked, zwitterionic, and isocytosine-isoguanine components. Methods. 2001 Feb; 23(2):141-8.
Switzer C, Chaput JC. PMID: 11181033.
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PubMed Mentions: Fields:
Translation:
Cells
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Nonenzymatic oligomerization on templates containing phosphodiester-linked acyclic glycerol nucleic acid analogues. J Mol Evol. 2000 Nov; 51(5):464-70.
Chaput JC, Switzer C. PMID: 11080369.
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PubMed Mentions:
6 Fields:
Translation:
Cells
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RecA protein promotes strand exchange with DNA substrates containing isoguanine and 5-methyl isocytosine. Biochemistry. 2000 Aug 22; 39(33):10177-88.
Rice KP, Chaput JC, Cox MM, Switzer C. PMID: 10956007.
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PubMed Mentions:
3 Fields:
Translation:
Cells
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Pretreatment expression of the perforin gene by circulating CD8(+) T lymphocytes predicts biochemical response to interferon-alpha in patients with chronic hepatitis C. Eur Cytokine Netw. 2000 Jun; 11(2):177-84.
Balian A, Naveau S, Zou W, Durand-Gasselin I, Bouchet L, Foussat A, Galanaud P, Chaput JC, Emilie D. PMID: 10903796.
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PubMed Mentions:
1 Fields:
Translation:
HumansCells
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A DNA pentaplex incorporating nucleobase quintets. Proc Natl Acad Sci U S A. 1999 Sep 14; 96(19):10614-9.
Chaput JC, Switzer C. PMID: 10485874; PMCID: PMC17931.
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PubMed Mentions:
15 Fields:
Translation:
Cells
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Beyond guanine quartets: cation-induced formation of homogenous and chimeric DNA tetraplexes incorporating iso-guanine and guanine. Chem Biol. 1997 Dec; 4(12):899-908.
Roberts C, Chaput JC, Switzer C. PMID: 9427655.
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PubMed Mentions:
7 Fields: