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Poulos, Thomas

Title(s)Chancellor's Professor, Molecular Biology & Biochemistry
Phone(949) 824-7020, 4322, 2856
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    Collapse Research 
    Collapse Research Activities and Funding
    Metalloenzyme structure, function, and as targets for neurodegeneration and bacterial pathogenesis
    NIH/NIGMS R35GM131920Jun 1, 2019 - May 31, 2024
    Role: Principal Investigator
    Training Program in Chemical and Structural Biology
    NIH/NIGMS T32GM108561Jul 1, 2014 - Jun 30, 2019
    Role: Principal Investigator
    Acquisition of a Bruker X8 Prospector Protein X-ray Crystallography System
    NIH/NCRR S10RR024679May 7, 2009 - May 6, 2010
    Role: Principal Investigator
    Nitroxyl adducts as structural probes of oxygenase/substrate interactions
    NIH/NIEHS R21ES016441Jan 1, 2008 - Dec 31, 2009
    Role: Principal Investigator
    Structural Studies on Nitric Oxide Synthase
    NIH/NIGMS R01GM057353May 1, 1998 - Aug 31, 2019
    Role: Principal Investigator
    7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEIN
    NIH/NIGMS R01GM045209Jan 1, 1991 - Dec 31, 2003
    Role: Principal Investigator
    NITROGENASE MECHANISM AND ASSEMBLY
    NIH/NIGMS R01GM043144Dec 1, 1989 - Mar 31, 2004
    Role: Principal Investigator
    Diversity in Heme Protein Structure and Function
    NIH/NIGMS R01GM042614Jul 1, 1989 - Mar 31, 2017
    Role: Principal Investigator
    Structural Studies on Cytochromes P450 and Related Proteins
    NIH/NIGMS R01GM033688Mar 1, 1985 - Nov 30, 2014
    Role: Principal Investigator
    X-RAY STUDIES OF PROTEIN STRUCTURE
    NIH/NIGMS R01GM033325Aug 1, 1983 - Jul 31, 1986
    Role: Principal Investigator

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    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Lamb DC, Follmer AH, Goldstone JV, Nelson DR, Warrilow AG, Price CL, True MY, Kelly SL, Poulos TL, Stegeman JJ. On the occurrence of cytochrome P450 in viruses. Proc Natl Acad Sci U S A. 2019 Jun 18; 116(25):12343-12352. PMID: 31167942.
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    2. Do HT, Li H, Chreifi G, Poulos TL, Silverman RB. Optimization of Blood-Brain Barrier Permeability with Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibitors Having a 2-Aminopyridine Scaffold. J Med Chem. 2019 Mar 14; 62(5):2690-2707. PMID: 30802056.
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    3. Follmer AH, Tripathi S, Poulos TL. Ligand and Redox Partner Binding Generates a New Conformational State in Cytochrome P450cam (CYP101A1). J Am Chem Soc. 2019 Feb 13; 141(6):2678-2683. PMID: 30672701.
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    4. Cinelli MA, Li H, Chreifi G, Poulos TL, Silverman RB. Correction to Nitrile in the Hole: Discovery of a Small Auxiliary Pocket in Neuronal Nitric Oxide Synthase Leading to the Development of Potent and Selective 2-Aminoquinoline Inhibitors. J Med Chem. 2019 Jan 11. PMID: 30633512.
      View in: PubMed
    5. Do HT, Wang HY, Li H, Chreifi G, Poulos TL, Silverman RB. Correction to Improvement of Cell Permeability of Human Neuronal Nitric Oxide Synthase Inhibitors Using Potent and Selective 2-Aminopyridine-Based Scaffolds with a Fluorobenzene Linker. J Med Chem. 2019 Jan 24; 62(2):1074. PMID: 30592215.
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    6. Follmer AH, Mahomed M, Goodin DB, Poulos TL. Substrate-Dependent Allosteric Regulation in Cytochrome P450cam (CYP101A1). J Am Chem Soc. 2018 Nov 28; 140(47):16222-16228. PMID: 30376314.
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    7. Li H, Evenson RJ, Chreifi G, Silverman RB, Poulos TL. Structural Basis for Isoform Selective Nitric Oxide Synthase Inhibition by Thiophene-2-carboximidamides. Biochemistry. 2018 Nov 06; 57(44):6319-6325. PMID: 30335983.
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    8. Lewis-Ballester A, Karkashon S, Batabyal D, Poulos TL, Yeh SR. Inhibition Mechanisms of Human Indoleamine 2,3 Dioxygenase 1. J Am Chem Soc. 2018 Jul 11; 140(27):8518-8525. PMID: 29897749.
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    9. Tripathi S, Poulos TL. Testing the N-Terminal Velcro Model of CooA Carbon Monoxide Activation. Biochemistry. 2018 05 29; 57(21):3059-3064. PMID: 29708736.
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    10. Batabyal D, Poulos TL. Effect of redox partner binding on CYP101D1 conformational dynamics. J Inorg Biochem. 2018 06; 183:179-183. PMID: 29550100.
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    11. Lewis-Ballester A, Pham KN, Batabyal D, Karkashon S, Bonanno JB, Poulos TL, Yeh SR. Structural insights into substrate and inhibitor binding sites in human indoleamine 2,3-dioxygenase 1. Nat Commun. 2017 11 22; 8(1):1693. PMID: 29167421.
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    12. Do HT, Wang HY, Li H, Chreifi G, Poulos TL, Silverman RB. Improvement of Cell Permeability of Human Neuronal Nitric Oxide Synthase Inhibitors Using Potent and Selective 2-Aminopyridine-Based Scaffolds with a Fluorobenzene Linker. J Med Chem. 2017 11 22; 60(22):9360-9375. PMID: 29091437.
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    13. Benabbas A, Sun Y, Poulos TL, Champion PM. Ultrafast CO Kinetics in Heme Proteins: Adiabatic Ligand Binding and Heavy Atom Tunneling. J Am Chem Soc. 2017 11 08; 139(44):15738-15747. PMID: 28984134.
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    14. Guo Z, Sevrioukova IF, Denisov IG, Zhang X, Chiu TL, Thomas DG, Hanse EA, Cuellar RAD, Grinkova YV, Langenfeld VW, Swedien DS, Stamschror JD, Alvarez J, Luna F, Galván A, Bae YK, Wulfkuhle JD, Gallagher RI, Petricoin EF, Norris B, Flory CM, Schumacher RJ, O'Sullivan MG, Cao Q, Chu H, Lipscomb JD, Atkins WM, Gupta K, Kelekar A, Blair IA, Capdevila JH, Falck JR, Sligar SG, Poulos TL, Georg GI, Ambrose E, Potter DA. Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria. Cell Chem Biol. 2017 10 19; 24(10):1314. PMID: 29053951.
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    15. Guo Z, Sevrioukova IF, Denisov IG, Zhang X, Chiu TL, Thomas DG, Hanse EA, Cuellar RAD, Grinkova YV, Langenfeld VW, Swedien DS, Stamschror JD, Alvarez J, Luna F, Galván A, Bae YK, Wulfkuhle JD, Gallagher RI, Petricoin EF, Norris B, Flory CM, Schumacher RJ, O'Sullivan MG, Cao Q, Chu H, Lipscomb JD, Atkins WM, Gupta K, Kelekar A, Blair IA, Capdevila JH, Falck JR, Sligar SG, Poulos TL, Georg GI, Ambrose E, Potter DA. Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria. Cell Chem Biol. 2017 Oct 19; 24(10):1259-1275.e6. PMID: 28919040.
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    16. Hollingsworth SA, Nguyen BD, Chreifi G, Arce AP, Poulos TL. Insights into the Dynamics and Dissociation Mechanism of a Protein Redox Complex Using Molecular Dynamics. J Chem Inf Model. 2017 09 25; 57(9):2344-2350. PMID: 28841378.
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    17. Batabyal D, Richards LS, Poulos TL. Effect of Redox Partner Binding on Cytochrome P450 Conformational Dynamics. J Am Chem Soc. 2017 09 20; 139(37):13193-13199. PMID: 28823160.
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    18. Pensa AV, Cinelli MA, Li H, Chreifi G, Mukherjee P, Roman LJ, Martásek P, Poulos TL, Silverman RB. Hydrophilic, Potent, and Selective 7-Substituted 2-Aminoquinolines as Improved Human Neuronal Nitric Oxide Synthase Inhibitors. J Med Chem. 2017 08 24; 60(16):7146-7165. PMID: 28776992.
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    19. Meneghini LM, Tripathi S, Woodworth MA, Majumdar S, Poulos TL, Weiss GA. Dissecting binding of a ß-barrel membrane protein by phage display. Mol Biosyst. 2017 Jul 25; 13(8):1438-1447. PMID: 28627567.
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    20. Chreifi G, Dejam D, Poulos TL. Crystal structure and functional analysis of Leishmania major pseudoperoxidase. J Biol Inorg Chem. 2017 Aug; 22(6):919-927. PMID: 28584975.
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    21. Cinelli MA, Li H, Chreifi G, Poulos TL, Silverman RB. Nitrile in the Hole: Discovery of a Small Auxiliary Pocket in Neuronal Nitric Oxide Synthase Leading to the Development of Potent and Selective 2-Aminoquinoline Inhibitors. J Med Chem. 2017 05 11; 60(9):3958-3978. PMID: 28422508.
      View in: PubMed
    22. Sevrioukova IF, Poulos TL. Structural basis for regiospecific midazolam oxidation by human cytochrome P450 3A4. Proc Natl Acad Sci U S A. 2017 01 17; 114(3):486-491. PMID: 28031486.
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    23. Poulos TL, Li H. Nitric oxide synthase and structure-based inhibitor design. Nitric Oxide. 2017 Feb 28; 63:68-77. PMID: 27890696.
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    24. Batabyal D, Lewis-Ballester A, Yeh SR, Poulos TL. A Comparative Analysis of the Effector Role of Redox Partner Binding in Bacterial P450s. Biochemistry. 2016 Nov 29; 55(47):6517-6523. PMID: 27808504.
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    25. Holden JK, Lewis MC, Cinelli MA, Abdullatif Z, Pensa AV, Silverman RB, Poulos TL. Targeting Bacterial Nitric Oxide Synthase with Aminoquinoline-Based Inhibitors. Biochemistry. 2016 Oct 04; 55(39):5587-5594. PMID: 27607918.
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    26. Hollingsworth SA, Batabyal D, Nguyen BD, Poulos TL. Conformational selectivity in cytochrome P450 redox partner interactions. Proc Natl Acad Sci U S A. 2016 08 02; 113(31):8723-8. PMID: 27439869; PMCID: PMC4978276 [Available on 02/02/17].
    27. Li H, Wang HY, Kang S, Silverman RB, Poulos TL. Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase. Biochemistry. 2016 07 05; 55(26):3702-7. PMID: 27250740.
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    28. Wang HY, Qin Y, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibition by Optimization of the 2-Aminopyridine-Based Scaffold with a Pyridine Linker. J Med Chem. 2016 05 26; 59(10):4913-25. PMID: 27050842; PMCID: PMC4882237 [Available on 05/26/17].
    29. Cinelli MA, Li H, Pensa AV, Kang S, Roman LJ, Martásek P, Poulos TL, Silverman RB. Correction to Phenyl Ether- and Aniline-Containing 2-Aminoquinolines as Potent and Selective Inhibitors of Neuronal Nitric Oxide Synthase. J Med Chem. 2016 Feb 11; 59(3):1246. PMID: 26807481; PMCID: PMC4957696.
    30. Chreifi G, Baxter EL, Doukov T, Cohen AE, McPhillips SE, Song J, Meharenna YT, Soltis SM, Poulos TL. Crystal structure of the pristine peroxidase ferryl center and its relevance to proton-coupled electron transfer. Proc Natl Acad Sci U S A. 2016 Feb 02; 113(5):1226-31. PMID: 26787871.
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    31. Fields JB, Hollingsworth SA, Chreifi G, Heyden M, Arce AP, Magaña-Garcia HI, Poulos TL, Tobias DJ. "Bind and Crawl" Association Mechanism of Leishmania major Peroxidase and Cytochrome c Revealed by Brownian and Molecular Dynamics Simulations. Biochemistry. 2015 Dec 15; 54(49):7272-82. PMID: 26598276.
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    32. Cinelli MA, Li H, Pensa AV, Kang S, Roman LJ, Martásek P, Poulos TL, Silverman RB. Phenyl Ether- and Aniline-Containing 2-Aminoquinolines as Potent and Selective Inhibitors of Neuronal Nitric Oxide Synthase. J Med Chem. 2015 Nov 12; 58(21):8694-712. PMID: 26469213.
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    33. Hollingsworth SA, Holden JK, Li H, Poulos TL. Elucidating nitric oxide synthase domain interactions by molecular dynamics. Protein Sci. 2016 Feb; 25(2):374-82. PMID: 26448477.
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    34. Kaur P, Chamberlin AR, Poulos TL, Sevrioukova IF. Structure-Based Inhibitor Design for Evaluation of a CYP3A4 Pharmacophore Model. J Med Chem. 2016 05 12; 59(9):4210-20. PMID: 26371436.
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    35. Kang S, Li H, Tang W, Martásek P, Roman LJ, Poulos TL, Silverman RB. 2-Aminopyridines with a Truncated Side Chain To Improve Human Neuronal Nitric Oxide Synthase Inhibitory Potency and Selectivity. J Med Chem. 2015 Jul 23; 58(14):5548-60. PMID: 26120733.
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    36. Holden JK, Dejam D, Lewis MC, Huang H, Kang S, Jing Q, Xue F, Silverman RB, Poulos TL. Inhibitor Bound Crystal Structures of Bacterial Nitric Oxide Synthase. Biochemistry. 2015 Jul 07; 54(26):4075-82. PMID: 26062720.
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    37. Sevrioukova IF, Poulos TL. Anion-Dependent Stimulation of CYP3A4 Monooxygenase. Biochemistry. 2015 Jul 07; 54(26):4083-96. PMID: 26066995.
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    38. Holden JK, Kang S, Beasley FC, Cinelli MA, Li H, Roy SG, Dejam D, Edinger AL, Nizet V, Silverman RB, Poulos TL. Nitric Oxide Synthase as a Target for Methicillin-Resistant Staphylococcus aureus. Chem Biol. 2015 Jun 18; 22(6):785-92. PMID: 26091171; PMCID: PMC4475277.
    39. Astashkin AV, Chen L, Elmore BO, Kunwar D, Miao Y, Li H, Poulos TL, Roman LJ, Feng C. Probing the Hydrogen Bonding of the Ferrous-NO Heme Center of nNOS by Pulsed Electron Paramagnetic Resonance. J Phys Chem A. 2015 Jun 25; 119(25):6641-9. PMID: 26035438.
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    40. Chreifi G, Hollingsworth SA, Li H, Tripathi S, Arce AP, Magaña-Garcia HI, Poulos TL. Enzymatic Mechanism of Leishmania major Peroxidase and the Critical Role of Specific Ionic Interactions. Biochemistry. 2015 Jun 02; 54(21):3328-36. PMID: 25941976.
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    41. Tang W, Li H, Doud EH, Chen Y, Choing S, Plaza C, Kelleher NL, Poulos TL, Silverman RB. Mechanism of Inactivation of Neuronal Nitric Oxide Synthase by (S)-2-Amino-5-(2-(methylthio)acetimidamido)pentanoic Acid. J Am Chem Soc. 2015 May 13; 137(18):5980-9. PMID: 25874809.
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    42. Tang W, Li H, Poulos TL, Silverman RB. Mechanistic studies of inactivation of inducible nitric oxide synthase by amidines. Biochemistry. 2015 Apr 21; 54(15):2530-8. PMID: 25811913.
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    43. Kaluka D, Batabyal D, Chiang BY, Poulos TL, Yeh SR. Spectroscopic and mutagenesis studies of human PGRMC1. Biochemistry. 2015 Mar 03; 54(8):1638-47. PMID: 25675345; PMCID: PMC4533898.
    44. Holden JK, Kang S, Hollingsworth SA, Li H, Lim N, Chen S, Huang H, Xue F, Tang W, Silverman RB, Poulos TL. Structure-based design of bacterial nitric oxide synthase inhibitors. J Med Chem. 2015 Jan 22; 58(2):994-1004. PMID: 25522110.
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    45. Sevrioukova IF, Poulos TL. Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions. Adv Exp Med Biol. 2015; 851:83-105. PMID: 26002732.
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    46. Mukherjee P, Li H, Sevrioukova I, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Novel 2,4-disubstituted pyrimidines as potent, selective, and cell-permeable inhibitors of neuronal nitric oxide synthase. J Med Chem. 2015 Feb 12; 58(3):1067-88. PMID: 25489882.
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    47. Hollingsworth SA, Poulos TL. Molecular dynamics of the P450cam-Pdx complex reveals complex stability and novel interface contacts. Protein Sci. 2015 Jan; 24(1):49-57. PMID: 25307478.
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    48. Li H, Jamal J, Plaza C, Pineda SH, Chreifi G, Jing Q, Cinelli MA, Silverman RB, Poulos TL. Structures of human constitutive nitric oxide synthases. Acta Crystallogr D Biol Crystallogr. 2014 Oct; 70(Pt 10):2667-74. PMID: 25286850.
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    49. Holden JK, Lim N, Poulos TL. Identification of redox partners and development of a novel chimeric bacterial nitric oxide synthase for structure activity analyses. J Biol Chem. 2014 Oct 17; 289(42):29437-45. PMID: 25194416.
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    50. Jing Q, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Combination of chiral linkers with thiophenecarboximidamide heads to improve the selectivity of inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem Lett. 2014 Sep 15; 24(18):4504-4510. PMID: 25149509.
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    51. Li H, Jamal J, Delker S, Plaza C, Ji H, Jing Q, Huang H, Kang S, Silverman RB, Poulos TL. The mobility of a conserved tyrosine residue controls isoform-dependent enzyme-inhibitor interactions in nitric oxide synthases. Biochemistry. 2014 Aug 19; 53(32):5272-9. PMID: 25089924.
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    52. Astashkin AV, Chen L, Zhou X, Li H, Poulos TL, Liu KJ, Guillemette JG, Feng C. Pulsed electron paramagnetic resonance study of domain docking in neuronal nitric oxide synthase: the calmodulin and output state perspective. J Phys Chem A. 2014 Aug 28; 118(34):6864-72. PMID: 25046446.
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    53. Chreifi G, Li H, McInnes CR, Gibson CL, Suckling CJ, Poulos TL. Communication between the zinc and tetrahydrobiopterin binding sites in nitric oxide synthase. Biochemistry. 2014 Jul 01; 53(25):4216-23. PMID: 24819538.
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    54. Kang S, Tang W, Li H, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Nitric oxide synthase inhibitors that interact with both heme propionate and tetrahydrobiopterin show high isoform selectivity. J Med Chem. 2014 May 22; 57(10):4382-96. PMID: 24758147.
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    55. Madrona Y, Hollingsworth SA, Tripathi S, Fields JB, Rwigema JC, Tobias DJ, Poulos TL. Crystal structure of cindoxin, the P450cin redox partner. Biochemistry. 2014 Mar 11; 53(9):1435-46. PMID: 24533927.
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    56. Cinelli MA, Li H, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Simplified 2-aminoquinoline-based scaffold for potent and selective neuronal nitric oxide synthase inhibition. J Med Chem. 2014 Feb 27; 57(4):1513-30. PMID: 24472039.
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    57. Huang H, Li H, Yang S, Chreifi G, Martásek P, Roman LJ, Meyskens FL, Poulos TL, Silverman RB. Potent and selective double-headed thiophene-2-carboximidamide inhibitors of neuronal nitric oxide synthase for the treatment of melanoma. J Med Chem. 2014 Feb 13; 57(3):686-700. PMID: 24447275.
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    58. Jing Q, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. An Accessible Chiral Linker to Enhance Potency and Selectivity of Neuronal Nitric Oxide Synthase Inhibitors. ACS Med Chem Lett. 2014 Jan 09; 5(1):56-60. PMID: 24660051.
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    59. Poulos TL. Heme enzyme structure and function. Chem Rev. 2014 Apr 09; 114(7):3919-62. PMID: 24400737; PMCID: PMC3981943.
    60. Sevrioukova IF, Poulos TL. Ritonavir analogues as a probe for deciphering the cytochrome P450 3A4 inhibitory mechanism. Curr Top Med Chem. 2014; 14(11):1348-55. PMID: 24805065.
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    61. Batabyal D, Poulos TL. Crystal structures and functional characterization of wild-type CYP101D1 and its active site mutants. Biochemistry. 2013 Dec 10; 52(49):8898-906. PMID: 24261604.
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    62. Holden JK, Li H, Jing Q, Kang S, Richo J, Silverman RB, Poulos TL. Structural and biological studies on bacterial nitric oxide synthase inhibitors. Proc Natl Acad Sci U S A. 2013 Nov 05; 110(45):18127-31. PMID: 24145412.
      View in: PubMed
    63. Jing Q, Li H, Chreifi G, Roman LJ, Martásek P, Poulos TL, Silverman RB. Chiral linkers to improve selectivity of double-headed neuronal nitric oxide synthase inhibitors. Bioorg Med Chem Lett. 2013 Oct 15; 23(20):5674-9. PMID: 23993333; PMCID: PMC3790268.
    64. Batabyal D, Li H, Poulos TL. Synergistic effects of mutations in cytochrome P450cam designed to mimic CYP101D1. Biochemistry. 2013 Aug 13; 52(32):5396-402. PMID: 23865948.
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    65. Tripathi S, O'Neill MJ, Wilks A, Poulos TL. Crystal structure of the Pseudomonas aeruginosa cytoplasmic heme binding protein, Apo-PhuS. J Inorg Biochem. 2013 Nov; 128:131-6. PMID: 23973453.
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    66. Madrona Y, Hollingsworth SA, Khan B, Poulos TL. P450cin active site water: implications for substrate binding and solvent accessibility. Biochemistry. 2013 Jul 30; 52(30):5039-50. PMID: 23829586.
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    67. Sevrioukova IF, Poulos TL. Dissecting cytochrome P450 3A4-ligand interactions using ritonavir analogues. Biochemistry. 2013 Jul 02; 52(26):4474-81. PMID: 23746300.
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    68. Jing Q, Li H, Fang J, Roman LJ, Martásek P, Poulos TL, Silverman RB. In search of potent and selective inhibitors of neuronal nitric oxide synthase with more simple structures. Bioorg Med Chem. 2013 Sep 01; 21(17):5323-31. PMID: 23867386.
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    69. Tripathi S, Li H, Poulos TL. Structural basis for effector control and redox partner recognition in cytochrome P450. Science. 2013 Jun 07; 340(6137):1227-30. PMID: 23744947.
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    70. Sevrioukova IF, Poulos TL. Pyridine-substituted desoxyritonavir is a more potent inhibitor of cytochrome P450 3A4 than ritonavir. J Med Chem. 2013 May 09; 56(9):3733-41. PMID: 23586711.
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    71. Jansen Labby K, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Methylated N(?)-hydroxy-L-arginine analogues as mechanistic probes for the second step of the nitric oxide synthase-catalyzed reaction. Biochemistry. 2013 May 07; 52(18):3062-73. PMID: 23586781.
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    72. Huang H, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Structure-guided design of selective inhibitors of neuronal nitric oxide synthase. J Med Chem. 2013 Apr 11; 56(7):3024-32. PMID: 23451760.
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    73. Li H, Jamal J, Chreifi G, Venkatesh V, Abou-Ziab H, Poulos TL. Dissecting the kinetics of the NADP(+)-FADH2 charge transfer complex and flavin semiquinones in neuronal nitric oxide synthase. J Inorg Biochem. 2013 Jul; 124:1-10. PMID: 23567464; PMCID: PMC3716911.
    74. Li H, Li C, Zhou S, Poulos TL, Gershon PD. Domain-level rocking motion within a polymerase that translocates on single-stranded nucleic acid. Acta Crystallogr D Biol Crystallogr. 2013 Apr; 69(Pt 4):617-24. PMID: 23519670.
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    75. Yang Z, Misner B, Ji H, Poulos TL, Silverman RB, Meyskens FL, Yang S. Targeting nitric oxide signaling with nNOS inhibitors as a novel strategy for the therapy and prevention of human melanoma. Antioxid Redox Signal. 2013 Aug 10; 19(5):433-47. PMID: 23199242; PMCID: PMC3704054.
    76. Poulos TL, Madrona Y. Oxygen activation and redox partner binding in cytochromes P450. Biotechnol Appl Biochem. 2013 Jan-Feb; 60(1):128-33. PMID: 23587000.
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    77. Li H, Xue F, Kraus JM, Ji H, Labby KJ, Mataka J, Delker SL, Martásek P, Roman LJ, Poulos TL, Silverman RB. Cyclopropyl- and methyl-containing inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2013 Mar 01; 21(5):1333-43. PMID: 23352768.
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    78. Salerno JC, Ray K, Poulos T, Li H, Ghosh DK. Calmodulin activates neuronal nitric oxide synthase by enabling transitions between conformational states. FEBS Lett. 2013 Jan 04; 587(1):44-7. PMID: 23159936.
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    79. Jasion VS, Doukov T, Pineda SH, Li H, Poulos TL. Crystal structure of the Leishmania major peroxidase-cytochrome c complex. Proc Natl Acad Sci U S A. 2012 Nov 06; 109(45):18390-4. PMID: 23100535.
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    80. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Nat Biotechnol. 2012 Nov; 30(11):1143-8. PMID: 23086203; PMCID: PMC3699407.
    81. Poulos TL, Li H. Structural basis for isoform-selective inhibition in nitric oxide synthase. Acc Chem Res. 2013 Feb 19; 46(2):390-8. PMID: 23030042.
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    82. Jensen LM, Meharenna YT, Davidson VL, Poulos TL, Hedman B, Wilmot CM, Sarangi R. Geometric and electronic structures of the His-Fe(IV)=O and His-Fe(IV)-Tyr hemes of MauG. J Biol Inorg Chem. 2012 Dec; 17(8):1241-55. PMID: 23053529.
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    83. Sevrioukova IF, Poulos TL. Understanding the mechanism of cytochrome P450 3A4: recent advances and remaining problems. Dalton Trans. 2013 Mar 07; 42(9):3116-26. PMID: 23018626; PMCID: PMC3787833.
    84. Madrona Y, Tripathi S, Li H, Poulos TL. Crystal structures of substrate-free and nitrosyl cytochrome P450cin: implications for O(2) activation. Biochemistry. 2012 Aug 21; 51(33):6623-31. PMID: 22775403.
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    85. Huang H, Ji H, Li H, Jing Q, Labby KJ, Martásek P, Roman LJ, Poulos TL, Silverman RB. Selective monocationic inhibitors of neuronal nitric oxide synthase. Binding mode insights from molecular dynamics simulations. J Am Chem Soc. 2012 Jul 18; 134(28):11559-72. PMID: 22731813.
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    86. Benabbas A, Karunakaran V, Youn H, Poulos TL, Champion PM. Effect of DNA binding on geminate CO recombination kinetics in CO-sensing transcription factor CooA. J Biol Chem. 2012 Jun 22; 287(26):21729-40. PMID: 22544803.
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    87. Jasion VS, Poulos TL. Leishmania major peroxidase is a cytochrome c peroxidase. Biochemistry. 2012 Mar 27; 51(12):2453-60. PMID: 22372542.
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    88. Sevrioukova IF, Poulos TL. Interaction of human cytochrome P4503A4 with ritonavir analogs. Arch Biochem Biophys. 2012 Apr 15; 520(2):108-16. PMID: 22410611.
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    91. Xue F, Kraus JM, Labby KJ, Ji H, Mataka J, Xia G, Li H, Delker SL, Roman LJ, Martásek P, Poulos TL, Silverman RB. Improved synthesis of chiral pyrrolidine inhibitors and their binding properties to neuronal nitric oxide synthase. J Med Chem. 2011 Sep 22; 54(18):6399-403. PMID: 21809851; PMCID: PMC3174355.
    92. Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL. Crystal structure of Leishmania major peroxidase and characterization of the compound i tryptophan radical. J Biol Chem. 2011 Jul 15; 286(28):24608-15. PMID: 21566139.
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    93. Doukov T, Li H, Sharma A, Martell JD, Soltis SM, Silverman RB, Poulos TL. Temperature-dependent spin crossover in neuronal nitric oxide synthase bound with the heme-coordinating thioether inhibitors. J Am Chem Soc. 2011 Jun 01; 133(21):8326-34. PMID: 21534614.
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    94. Xue F, Fang J, Delker SL, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Symmetric double-headed aminopyridines, a novel strategy for potent and membrane-permeable inhibitors of neuronal nitric oxide synthase. J Med Chem. 2011 Apr 14; 54(7):2039-48. PMID: 21410186; PMCID: PMC3072459.
    95. Delker SL, Xue F, Li H, Jamal J, Silverman RB, Poulos TL. Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase . Biochemistry. 2010 Dec 28; 49(51):10803-10. PMID: 21138269.
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    96. Ji H, Delker SL, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Exploration of the active site of neuronal nitric oxide synthase by the design and synthesis of pyrrolidinomethyl 2-aminopyridine derivatives. J Med Chem. 2010 Nov 11; 53(21):7804-24. PMID: 20958055.
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    97. Xue F, Li H, Delker SL, Fang J, Martásek P, Roman LJ, Poulos TL, Silverman RB. Potent, highly selective, and orally bioavailable gem-difluorinated monocationic inhibitors of neuronal nitric oxide synthase. J Am Chem Soc. 2010 Oct 13; 132(40):14229-38. PMID: 20843082.
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    98. Sevrioukova IF, Poulos TL. Structure and mechanism of the complex between cytochrome P4503A4 and ritonavir. Proc Natl Acad Sci U S A. 2010 Oct 26; 107(43):18422-7. PMID: 20937904.
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    99. Sevrioukova IF, Poulos TL. Structural biology of redox partner interactions in P450cam monooxygenase: a fresh look at an old system. Arch Biochem Biophys. 2011 Mar 01; 507(1):66-74. PMID: 20816746; PMCID: PMC3193999.
    100. Xue F, Li H, Fang J, Roman LJ, Martásek P, Poulos TL, Silverman RB. Peripheral but crucial: a hydrophobic pocket (Tyr(706), Leu(337), and Met(336)) for potent and selective inhibition of neuronal nitric oxide synthase. Bioorg Med Chem Lett. 2010 Nov 01; 20(21):6258-61. PMID: 20833542.
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    101. Meharenna YT, Poulos TL. Using molecular dynamics to probe the structural basis for enhanced stability in thermal stable cytochromes P450. Biochemistry. 2010 Aug 10; 49(31):6680-6. PMID: 20593793.
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    102. Xue F, Huang J, Ji H, Fang J, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Structure-based design, synthesis, and biological evaluation of lipophilic-tailed monocationic inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2010 Sep 01; 18(17):6526-37. PMID: 20673724.
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    103. Sevrioukova IF, Poulos TL. Arginines 65 and 310 in putidaredoxin reductase are critical for interaction with putidaredoxin. Biochemistry. 2010 Jun 29; 49(25):5160-6. PMID: 20524621.
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    104. Delker SL, Ji H, Li H, Jamal J, Fang J, Xue F, Silverman RB, Poulos TL. Unexpected binding modes of nitric oxide synthase inhibitors effective in the prevention of a cerebral palsy phenotype in an animal model. J Am Chem Soc. 2010 Apr 21; 132(15):5437-42. PMID: 20337441.
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    105. Meharenna YT, Doukov T, Li H, Soltis SM, Poulos TL. Crystallographic and single-crystal spectral analysis of the peroxidase ferryl intermediate. Biochemistry. 2010 Apr 13; 49(14):2984-6. PMID: 20230048.
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    106. Sundaramoorthy M, Gold MH, Poulos TL. Ultrahigh (0.93A) resolution structure of manganese peroxidase from Phanerochaete chrysosporium: implications for the catalytic mechanism. J Inorg Biochem. 2010 Jun; 104(6):683-90. PMID: 20356630.
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    107. Poulos TL. Thirty years of heme peroxidase structural biology. Arch Biochem Biophys. 2010 Aug 01; 500(1):3-12. PMID: 20206121; PMCID: PMC3202974.
    108. Sevrioukova IF, Poulos TL, Churbanova IY. Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex. J Biol Chem. 2010 Apr 30; 285(18):13616-20. PMID: 20179327.
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    109. Martell JD, Li H, Doukov T, Martásek P, Roman LJ, Soltis M, Poulos TL, Silverman RB. Heme-coordinating inhibitors of neuronal nitric oxide synthase. Iron-thioether coordination is stabilized by hydrophobic contacts without increased inhibitor potency. J Am Chem Soc. 2010 Jan 20; 132(2):798-806. PMID: 20014790.
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    110. Churbanova IY, Poulos TL, Sevrioukova IF. Production and characterization of a functional putidaredoxin reductase-putidaredoxin covalent complex. Biochemistry. 2010 Jan 12; 49(1):58-67. PMID: 19954240.
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    111. Doukov T, Li H, Soltis M, Poulos TL. Single crystal structural and absorption spectral characterizations of nitric oxide synthase complexed with N(omega)-hydroxy-L-arginine and diatomic ligands. Biochemistry. 2009 Nov 03; 48(43):10246-54. PMID: 19791770; PMCID: PMC3740733.
    112. Ioanoviciu A, Meharenna YT, Poulos TL, Ortiz de Montellano PR. DevS oxy complex stability identifies this heme protein as a gas sensor in Mycobacterium tuberculosis dormancy. Biochemistry. 2009 Jun 30; 48(25):5839-48. PMID: 19463006.
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    113. Kumar MR, Pervitsky D, Chen L, Poulos T, Kundu S, Hargrove MS, Rivera EJ, Diaz A, Colón JL, Farmer PJ. Nitrosyl hydride (HNO) as an O2 analogue: long-lived HNO adducts of ferrous globins. Biochemistry. 2009 Jun 09; 48(22):5018-25. PMID: 19368336.
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    114. Li C, Li H, Zhou S, Sun E, Yoshizawa J, Poulos TL, Gershon PD. Polymerase translocation with respect to single-stranded nucleic acid: looping or wrapping of primer around a poly(A) polymerase. Structure. 2009 May 13; 17(5):680-9. PMID: 19446524.
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    115. Igarashi J, Li H, Jamal J, Ji H, Fang J, Lawton GR, Silverman RB, Poulos TL. Crystal structures of constitutive nitric oxide synthases in complex with de novo designed inhibitors. J Med Chem. 2009 Apr 09; 52(7):2060-6. PMID: 19296678.
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    116. Ji H, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Discovery of highly potent and selective inhibitors of neuronal nitric oxide synthase by fragment hopping. J Med Chem. 2009 Feb 12; 52(3):779-97. PMID: 19125620.
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    117. Ji H, Tan S, Igarashi J, Li H, Derrick M, Martásek P, Roman LJ, Vásquez-Vivar J, Poulos TL, Silverman RB. Selective neuronal nitric oxide synthase inhibitors and the prevention of cerebral palsy. Ann Neurol. 2009 Feb; 65(2):209-17. PMID: 19235180; PMCID: PMC2658647.
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    119. Meharenna YT, Oertel P, Bhaskar B, Poulos TL. Engineering ascorbate peroxidase activity into cytochrome c peroxidase. Biochemistry. 2008 Sep 30; 47(39):10324-32. PMID: 18771292.
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    120. Fasan R, Meharenna YT, Snow CD, Poulos TL, Arnold FH. Evolutionary history of a specialized p450 propane monooxygenase. J Mol Biol. 2008 Nov 28; 383(5):1069-80. PMID: 18619466.
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    121. Gruia F, Ionascu D, Kubo M, Ye X, Dawson J, Osborne RL, Sligar SG, Denisov I, Das A, Poulos TL, Terner J, Champion PM. Low-frequency dynamics of Caldariomyces fumago chloroperoxidase probed by femtosecond coherence spectroscopy. Biochemistry. 2008 May 06; 47(18):5156-67. PMID: 18407660.
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    122. Ji H, Stanton BZ, Igarashi J, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Minimal pharmacophoric elements and fragment hopping, an approach directed at molecular diversity and isozyme selectivity. Design of selective neuronal nitric oxide synthase inhibitors. J Am Chem Soc. 2008 Mar 26; 130(12):3900-14. PMID: 18321097.
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    123. Meharenna YT, Slessor KE, Cavaignac SM, Poulos TL, De Voss JJ. The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin. J Biol Chem. 2008 Apr 18; 283(16):10804-12. PMID: 18270198.
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    124. Ho WW, Li H, Nishida CR, de Montellano PR, Poulos TL. Crystal structure and properties of CYP231A2 from the thermoacidophilic archaeon Picrophilus torridus. Biochemistry. 2008 Feb 19; 47(7):2071-9. PMID: 18197710.
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    125. Ho WW, Li H, Eakanunkul S, Tong Y, Wilks A, Guo M, Poulos TL. Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins. J Biol Chem. 2007 Dec 07; 282(49):35796-802. PMID: 17925389.
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    126. Corbett MC, Latimer MJ, Poulos TL, Sevrioukova IF, Hodgson KO, Hedman B. Photoreduction of the active site of the metalloprotein putidaredoxin by synchrotron radiation. Acta Crystallogr D Biol Crystallogr. 2007 Sep; 63(Pt 9):951-60. PMID: 17704563.
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    127. Ibrahim M, Kuchinskas M, Youn H, Kerby RL, Roberts GP, Poulos TL, Spiro TG. Mechanism of the CO-sensing heme protein CooA: new insights from the truncated heme domain and UVRR spectroscopy. J Inorg Biochem. 2007 Nov; 101(11-12):1776-85. PMID: 17720248.
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    128. Kimmich N, Das A, Sevrioukova I, Meharenna Y, Sligar SG, Poulos TL. Electron transfer between cytochrome P450cin and its FMN-containing redox partner, cindoxin. J Biol Chem. 2007 Sep 14; 282(37):27006-11. PMID: 17606612.
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    129. Levin AM, Murase K, Jackson PJ, Flinspach ML, Poulos TL, Weiss GA. Double barrel shotgun scanning of the caveolin-1 scaffolding domain. ACS Chem Biol. 2007 Jul 20; 2(7):493-500. PMID: 17602618; PMCID: PMC2532506.
    130. Seo J, Igarashi J, Li H, Martasek P, Roman LJ, Poulos TL, Silverman RB. Structure-based design and synthesis of N(omega)-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water. J Med Chem. 2007 May 03; 50(9):2089-99. PMID: 17425297.
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    131. Poulos TL. The Janus nature of heme. Nat Prod Rep. 2007 Jun; 24(3):504-10. PMID: 17534526.
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    132. Borjigin M, Li H, Lanz ND, Kerby RL, Roberts GP, Poulos TL. Structure-based hypothesis on the activation of the CO-sensing transcription factor CooA. Acta Crystallogr D Biol Crystallogr. 2007 Mar; 63(Pt 3):282-7. PMID: 17327664.
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    133. Poulos TL. Structural biology of p450-oxy complexes. Drug Metab Rev. 2007; 39(2-3):557-66. PMID: 17786638.
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    134. Friedman J, Meharenna YT, Wilks A, Poulos TL. Diatomic ligand discrimination by the heme oxygenases from Neisseria meningitidis and Pseudomonas aeruginosa. J Biol Chem. 2007 Jan 12; 282(2):1066-71. PMID: 17095508.
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    135. Kuznetsov VY, Poulos TL, Sevrioukova IF. Putidaredoxin-to-cytochrome P450cam electron transfer: differences between the two reductive steps required for catalysis. Biochemistry. 2006 Oct 03; 45(39):11934-44. PMID: 17002293.
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    136. Poulos TL. Soluble guanylate cyclase. Curr Opin Struct Biol. 2006 Dec; 16(6):736-43. PMID: 17015012.
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    137. Li H, Igarashi J, Jamal J, Yang W, Poulos TL. Structural studies of constitutive nitric oxide synthases with diatomic ligands bound. J Biol Inorg Chem. 2006 Sep; 11(6):753-68. PMID: 16804678.
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    138. Kuchinskas M, Li H, Conrad M, Roberts G, Poulos TL. The role of the DNA-binding domains in CooA activation. Biochemistry. 2006 Jun 13; 45(23):7148-53. PMID: 16752905.
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    139. Swartz L, Kuchinskas M, Li H, Poulos TL, Lanzilotta WN. Redox-dependent structural changes in the Azotobacter vinelandii bacterioferritin: new insights into the ferroxidase and iron transport mechanism. Biochemistry. 2006 Apr 11; 45(14):4421-8. PMID: 16584178.
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    140. Mbadugha BN, Seo J, Ji H, Martásek P, Roman LJ, Shea TM, Li H, Poulos TL, Silverman RB. Hydroxyl-terminated peptidomimetic inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2006 Jun 01; 14(11):3681-90. PMID: 16480878.
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    141. Li H, Flinspach ML, Igarashi J, Jamal J, Yang W, Gómez-Vidal JA, Litzinger EA, Huang H, Erdal EP, Silverman RB, Poulos TL. Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases. Biochemistry. 2005 Nov 22; 44(46):15222-9. PMID: 16285725.
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    142. Barrows TP, Poulos TL. Role of electrostatics and salt bridges in stabilizing the compound I radical in ascorbate peroxidase. Biochemistry. 2005 Nov 01; 44(43):14062-8. PMID: 16245922.
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    143. Poulos TL. Structural biology of heme monooxygenases. Biochem Biophys Res Commun. 2005 Dec 09; 338(1):337-45. PMID: 16185651.
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    144. Nagano S, Poulos TL. Crystallographic study on the dioxygen complex of wild-type and mutant cytochrome P450cam. Implications for the dioxygen activation mechanism. J Biol Chem. 2005 Sep 09; 280(36):31659-63. PMID: 15994329.
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    145. Bhaskar B, Poulos TL. The 1.13-A structure of iron-free cytochrome c peroxidase. J Biol Inorg Chem. 2005 Jun; 10(4):425-30. PMID: 15900441.
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    146. Sundaramoorthy M, Youngs HL, Gold MH, Poulos TL. High-resolution crystal structure of manganese peroxidase: substrate and inhibitor complexes. Biochemistry. 2005 May 03; 44(17):6463-70. PMID: 15850380.
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    147. Poulos TL. Intermediates in P450 catalysis. Philos Trans A Math Phys Eng Sci. 2005 Apr 15; 363(1829):793-806; discussion 1035-40. PMID: 15901536.
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    148. Nagano S, Cupp-Vickery JR, Poulos TL. Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF. J Biol Chem. 2005 Jun 10; 280(23):22102-7. PMID: 15824115.
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    149. Kuznetsov VY, Blair E, Farmer PJ, Poulos TL, Pifferitti A, Sevrioukova IF. The putidaredoxin reductase-putidaredoxin electron transfer complex: theoretical and experimental studies. J Biol Chem. 2005 Apr 22; 280(16):16135-42. PMID: 15716266.
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    150. Lad L, Koshkin A, de Montellano PR, Poulos TL. Crystal structures of the G139A, G139A-NO and G143H mutants of human heme oxygenase-1. A finely tuned hydrogen-bonding network controls oxygenase versus peroxidase activity. J Biol Inorg Chem. 2005 Mar; 10(2):138-46. PMID: 15690204.
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    151. Li H, Poulos TL. Structure-function studies on nitric oxide synthases. J Inorg Biochem. 2005 Jan; 99(1):293-305. PMID: 15598508.
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    152. Wang J, Lad L, Poulos TL, Ortiz de Montellano PR. Regiospecificity determinants of human heme oxygenase: differential NADPH- and ascorbate-dependent heme cleavage by the R183E mutant. J Biol Chem. 2005 Jan 28; 280(4):2797-806. PMID: 15525643.
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    153. Lad L, Ortiz de Montellano PR, Poulos TL. Crystal structures of ferrous and ferrous-NO forms of verdoheme in a complex with human heme oxygenase-1: catalytic implications for heme cleavage. J Inorg Biochem. 2004 Nov; 98(11):1686-95. PMID: 15522396.
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    154. Poulos TL. Structural and functional diversity in heme monooxygenases. Drug Metab Dispos. 2005 Jan; 33(1):10-8. PMID: 15475411.
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    155. Wang J, Niemevz F, Lad L, Huang L, Alvarez DE, Buldain G, Poulos TL, de Montellano PR. Human heme oxygenase oxidation of 5- and 15-phenylhemes. J Biol Chem. 2004 Oct 08; 279(41):42593-604. PMID: 15297453.
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    156. Meharenna YT, Li H, Hawkes DB, Pearson AG, De Voss J, Poulos TL. Crystal structure of P450cin in a complex with its substrate, 1,8-cineole, a close structural homologue to D-camphor, the substrate for P450cam. Biochemistry. 2004 Jul 27; 43(29):9487-94. PMID: 15260491.
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    157. Nagano S, Tosha T, Ishimori K, Morishima I, Poulos TL. Crystal structure of the cytochrome p450cam mutant that exhibits the same spectral perturbations induced by putidaredoxin binding. J Biol Chem. 2004 Oct 08; 279(41):42844-9. PMID: 15269210.
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    158. Barrows TP, Bhaskar B, Poulos TL. Electrostatic control of the tryptophan radical in cytochrome c peroxidase. Biochemistry. 2004 Jul 13; 43(27):8826-34. PMID: 15236591.
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    159. Flinspach M, Li H, Jamal J, Yang W, Huang H, Silverman RB, Poulos TL. Structures of the neuronal and endothelial nitric oxide synthase heme domain with D-nitroarginine-containing dipeptide inhibitors bound. Biochemistry. 2004 May 11; 43(18):5181-7. PMID: 15122883.
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    160. Friedman J, Lad L, Li H, Wilks A, Poulos TL. Structural basis for novel delta-regioselective heme oxygenation in the opportunistic pathogen Pseudomonas aeruginosa. Biochemistry. 2004 May 11; 43(18):5239-45. PMID: 15122889.
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    161. Guo M, Bhaskar B, Li H, Barrows TP, Poulos TL. Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link. Proc Natl Acad Sci U S A. 2004 Apr 20; 101(16):5940-5. PMID: 15071191.
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    162. Lad L, Friedman J, Li H, Bhaskar B, Ortiz de Montellano PR, Poulos TL. Crystal structure of human heme oxygenase-1 in a complex with biliverdin. Biochemistry. 2004 Apr 06; 43(13):3793-801. PMID: 15049686.
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    163. Sevrioukova IF, Li H, Poulos TL. Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase. J Mol Biol. 2004 Feb 27; 336(4):889-902. PMID: 15095867.
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    164. Li H, Poulos TL. Crystallization of cytochromes P450 and substrate-enzyme interactions. Curr Top Med Chem. 2004; 4(16):1789-802. PMID: 15579108.
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    165. Flinspach ML, Li H, Jamal J, Yang W, Huang H, Hah JM, Gómez-Vidal JA, Litzinger EA, Silverman RB, Poulos TL. Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase. Nat Struct Mol Biol. 2004 Jan; 11(1):54-9. PMID: 14718923.
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    166. Ji H, Li H, Flinspach M, Poulos TL, Silverman RB. Computer modeling of selective regions in the active site of nitric oxide synthases: implication for the design of isoform-selective inhibitors. J Med Chem. 2003 Dec 18; 46(26):5700-11. PMID: 14667223.
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    167. Poulos TL. The past and present of P450cam structural biology. Biochem Biophys Res Commun. 2003 Dec 05; 312(1):35-9. PMID: 14630013.
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    168. Poulos TL. Cytochrome P450 flexibility. Proc Natl Acad Sci U S A. 2003 Nov 11; 100(23):13121-2. PMID: 14597705; PMCID: PMC263725.
    169. Sevrioukova IF, Garcia C, Li H, Bhaskar B, Poulos TL. Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida. J Mol Biol. 2003 Oct 17; 333(2):377-92. PMID: 14529624.
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    170. Bretscher LE, Li H, Poulos TL, Griffith OW. Structural characterization and kinetics of nitric-oxide synthase inhibition by novel N5-(iminoalkyl)- and N5-(iminoalkenyl)-ornithines. J Biol Chem. 2003 Nov 21; 278(47):46789-97. PMID: 12960153.
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    171. Nagano S, Li H, Shimizu H, Nishida C, Ogura H, Ortiz de Montellano PR, Poulos TL. Crystal structures of epothilone D-bound, epothilone B-bound, and substrate-free forms of cytochrome P450epoK. J Biol Chem. 2003 Nov 07; 278(45):44886-93. PMID: 12933799.
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    172. Yano JK, Poulos TL. New understandings of thermostable and peizostable enzymes. Curr Opin Biotechnol. 2003 Aug; 14(4):360-5. PMID: 12943843.
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    173. Lad L, Wang J, Li H, Friedman J, Bhaskar B, Ortiz de Montellano PR, Poulos TL. Crystal structures of the ferric, ferrous, and ferrous-NO forms of the Asp140Ala mutant of human heme oxygenase-1: catalytic implications. J Mol Biol. 2003 Jul 11; 330(3):527-38. PMID: 12842469.
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    174. Friedman J, Lad L, Deshmukh R, Li H, Wilks A, Poulos TL. Crystal structures of the NO- and CO-bound heme oxygenase from Neisseriae meningitidis. Implications for O2 activation. J Biol Chem. 2003 Sep 05; 278(36):34654-9. PMID: 12819228.
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    175. Jia Q, Cai T, Huang M, Li H, Xian M, Poulos TL, Wang PG. Isoform-selective substrates of nitric oxide synthase. J Med Chem. 2003 Jun 05; 46(12):2271-4. PMID: 12773030.
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    176. Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL. High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase. Biochemistry. 2003 May 20; 42(19):5600-8. PMID: 12741816.
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    177. Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL. A novel heme and peroxide-dependent tryptophan-tyrosine cross-link in a mutant of cytochrome c peroxidase. J Mol Biol. 2003 Apr 18; 328(1):157-66. PMID: 12684005.
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    178. Lad L, Schuller DJ, Shimizu H, Friedman J, Li H, Ortiz de Montellano PR, Poulos TL. Comparison of the heme-free and -bound crystal structures of human heme oxygenase-1. J Biol Chem. 2003 Mar 07; 278(10):7834-43. PMID: 12500973.
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    179. Li H, Shimizu H, Flinspach M, Jamal J, Yang W, Xian M, Cai T, Wen EZ, Jia Q, Wang PG, Poulos TL. The novel binding mode of N-alkyl-N'-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO biosynthesis. Biochemistry. 2002 Nov 26; 41(47):13868-75. PMID: 12437343.
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    180. Yano JK, Blasco F, Li H, Schmid RD, Henne A, Poulos TL. Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus. J Biol Chem. 2003 Jan 03; 278(1):608-16. PMID: 12401810.
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    181. Immoos CE, Bhaskar B, Cohen MS, Barrows TP, Farmer PJ, Poulos TL. Mesopone cytochrome c peroxidase: functional model of heme oxygenated oxidases. J Inorg Biochem. 2002 Sep 20; 91(4):635-43. PMID: 12237229.
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    182. Sevrioukova IF, Poulos TL. Putidaredoxin reductase, a new function for an old protein. J Biol Chem. 2002 Jul 12; 277(28):25831-9. PMID: 12011076.
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    183. Bhaskar B, Bonagura CA, Li H, Poulos TL. Cation-induced stabilization of the engineered cation-binding loop in cytochrome c peroxidase (CcP). Biochemistry. 2002 Feb 26; 41(8):2684-93. PMID: 11851415.
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    184. Li H, Yano JK, Poulos TL. Molecular replacement in P450 crystal structure determinations. Methods Enzymol. 2002; 357:79-93. PMID: 12424900.
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    185. Podust LM, Stojan J, Poulos TL, Waterman MR. Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51. J Inorg Biochem. 2001 Dec 15; 87(4):227-35. PMID: 11744060.
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    186. Raman CS, Li H, Martásek P, Southan G, Masters BS, Poulos TL. Crystal structure of nitric oxide synthase bound to nitro indazole reveals a novel inactivation mechanism. Biochemistry. 2001 Nov 13; 40(45):13448-55. PMID: 11695891.
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    187. Shimizu H, Schuller DJ, Lanzilotta WN, Sundaramoorthy M, Arciero DM, Hooper AB, Poulos TL. Crystal structure of Nitrosomonas europaea cytochrome c peroxidase and the structural basis for ligand switching in bacterial di-heme peroxidases. Biochemistry. 2001 Nov 13; 40(45):13483-90. PMID: 11695895.
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    188. Kotsonis P, Fröhlich LG, Raman CS, Li H, Berg M, Gerwig R, Groehn V, Kang Y, Al-Masoudi N, Taghavi-Moghadam S, Mohr D, Münch U, Schnabel J, Martásek P, Masters BS, Strobel H, Poulos T, Matter H, Pfleiderer W, Schmidt HH. Structural basis for pterin antagonism in nitric-oxide synthase. Development of novel 4-oxo-pteridine antagonists of (6R)-5,6,7,8-tetrahydrobiopterin. J Biol Chem. 2001 Dec 28; 276(52):49133-41. PMID: 11590164.
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    189. Schuller DJ, Zhu W, Stojiljkovic I, Wilks A, Poulos TL. Crystal structure of heme oxygenase from the gram-negative pathogen Neisseria meningitidis and a comparison with mammalian heme oxygenase-1. Biochemistry. 2001 Sep 25; 40(38):11552-8. PMID: 11560504.
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    190. Sevrioukova IF, Hazzard JT, Tollin G, Poulos TL. Laser flash induced electron transfer in P450cam monooxygenase: putidaredoxin reductase-putidaredoxin interaction. Biochemistry. 2001 Sep 04; 40(35):10592-600. PMID: 11524002.
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    191. Li H, Raman CS, Martásek P, Masters BS, Poulos TL. Crystallographic studies on endothelial nitric oxide synthase complexed with nitric oxide and mechanism-based inhibitors. Biochemistry. 2001 May 08; 40(18):5399-406. PMID: 11331003.
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    192. Raman CS, Li H, Martásek P, Babu BR, Griffith OW, Masters BS, Poulos TL. Implications for isoform-selective inhibitor design derived from the binding mode of bulky isothioureas to the heme domain of endothelial nitric-oxide synthase. J Biol Chem. 2001 Jul 13; 276(28):26486-91. PMID: 11331290.
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    193. Podust LM, Poulos TL, Waterman MR. Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors. Proc Natl Acad Sci U S A. 2001 Mar 13; 98(6):3068-73. PMID: 11248033.
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    194. Roberts GP, Thorsteinsson MV, Kerby RL, Lanzilotta WN, Poulos T. CooA: a heme-containing regulatory protein that serves as a specific sensor of both carbon monoxide and redox state. Prog Nucleic Acid Res Mol Biol. 2001; 67:35-63. PMID: 11525385.
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    195. Lightning LK, Huang H, Moenne-Loccoz P, Loehr TM, Schuller DJ, Poulos TL, de Montellano PR. Disruption of an active site hydrogen bond converts human heme oxygenase-1 into a peroxidase. J Biol Chem. 2001 Apr 06; 276(14):10612-9. PMID: 11121422.
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    196. Liu Y, Koenigs Lightning L, Huang H, Moënne-Loccoz P, Schuller DJ, Poulos TL, Loehr TM, Ortiz de Montellano PR. Replacement of the distal glycine 139 transforms human heme oxygenase-1 into a peroxidase. J Biol Chem. 2000 Nov 03; 275(44):34501-7. PMID: 10942763.
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    197. Yano JK, Koo LS, Schuller DJ, Li H, Ortiz de Montellano PR, Poulos TL. Crystal structure of a thermophilic cytochrome P450 from the archaeon Sulfolobus solfataricus. J Biol Chem. 2000 Oct 06; 275(40):31086-92. PMID: 10859321.
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    198. Lanzilotta WN, Schuller DJ, Thorsteinsson MV, Kerby RL, Roberts GP, Poulos TL. Structure of the CO sensing transcription activator CooA. Nat Struct Biol. 2000 Oct; 7(10):876-80. PMID: 11017196.
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    199. Li H, Raman CS, Martásek P, Král V, Masters BS, Poulos TL. Mapping the active site polarity in structures of endothelial nitric oxide synthase heme domain complexed with isothioureas. J Inorg Biochem. 2000 Aug 31; 81(3):133-9. PMID: 11051558.
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    200. Satterlee JD, Teske JG, Erman JE, Mauro JM, Poulos TL. Temperature, pH, and solvent isotope effects on cytochrome c peroxidase mutant N82A studied by proton NMR. J Protein Chem. 2000 Aug; 19(6):535-42. PMID: 11195979.
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    201. Bursey EH, Poulos TL. Two substrate binding sites in ascorbate peroxidase: the role of arginine 172. Biochemistry. 2000 Jun 27; 39(25):7374-9. PMID: 10858284.
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    202. Bonagura CA, Bhaskar B, Sundaramoorthy M, Poulos TL. Conversion of an engineered potassium-binding site into a calcium-selective site in cytochrome c peroxidase. J Biol Chem. 1999 Dec 31; 274(53):37827-33. PMID: 10608846.
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    203. Sevrioukova IF, Hazzard JT, Tollin G, Poulos TL. The FMN to heme electron transfer in cytochrome P450BM-3. Effect of chemical modification of cysteines engineered at the FMN-heme domain interaction site. J Biol Chem. 1999 Dec 17; 274(51):36097-106. PMID: 10593892.
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    204. Li H, Poulos TL. Fatty acid metabolism, conformational change, and electron transfer in cytochrome P-450(BM-3). Biochim Biophys Acta. 1999 Nov 23; 1441(2-3):141-9. PMID: 10570242.
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    205. Schuller DJ, Wilks A, Ortiz de Montellano PR, Poulos TL. Crystal structure of human heme oxygenase-1. Nat Struct Biol. 1999 Sep; 6(9):860-7. PMID: 10467099.
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    206. Poulos TL. Helping copper find a home. Nat Struct Biol. 1999 Aug; 6(8):709-11. PMID: 10426941.
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    207. Li H, Raman CS, Glaser CB, Blasko E, Young TA, Parkinson JF, Whitlow M, Poulos TL. Crystal structures of zinc-free and -bound heme domain of human inducible nitric-oxide synthase. Implications for dimer stability and comparison with endothelial nitric-oxide synthase. J Biol Chem. 1999 Jul 23; 274(30):21276-84. PMID: 10409685.
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    208. Webster SD, Tenner AJ, Poulos TL, Cribbs DH. The mouse C1q A-chain sequence alters beta-amyloid-induced complement activation. Neurobiol Aging. 1999 May-Jun; 20(3):297-304. PMID: 10588577.
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    209. Bonagura CA, Sundaramoorthy M, Bhaskar B, Poulos TL. The effects of an engineered cation site on the structure, activity, and EPR properties of cytochrome c peroxidase. Biochemistry. 1999 Apr 27; 38(17):5538-45. PMID: 10220341.
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    210. Poulos TL, Li H, Raman CS. Heme-mediated oxygen activation in biology: cytochrome c oxidase and nitric oxide synthase. Curr Opin Chem Biol. 1999 Apr; 3(2):131-7. PMID: 10348620.
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    211. Sevrioukova IF, Li H, Zhang H, Peterson JA, Poulos TL. Structure of a cytochrome P450-redox partner electron-transfer complex. Proc Natl Acad Sci U S A. 1999 Mar 02; 96(5):1863-8. PMID: 10051560.
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    212. Cheek J, Mandelman D, Poulos TL, Dawson JH. A study of the K(+)-site mutant of ascorbate peroxidase: mutations of protein residues on the proximal side of the heme cause changes in iron ligation on the distal side. J Biol Inorg Chem. 1999 Feb; 4(1):64-72. PMID: 10499104.
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    213. Raman CS, Li H, Martásek P, Král V, Masters BS, Poulos TL. Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center. Cell. 1998 Dec 23; 95(7):939-50. PMID: 9875848.
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    214. Mandelman D, Jamal J, Poulos TL. Identification of two electron-transfer sites in ascorbate peroxidase using chemical modification, enzyme kinetics, and crystallography. Biochemistry. 1998 Dec 15; 37(50):17610-7. PMID: 9860877.
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    215. Mandelman D, Schwarz FP, Li H, Poulos TL. The role of quaternary interactions on the stability and activity of ascorbate peroxidase. Protein Sci. 1998 Oct; 7(10):2089-98. PMID: 9792095.
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    216. Sundaramoorthy M, Terner J, Poulos TL. Stereochemistry of the chloroperoxidase active site: crystallographic and molecular-modeling studies. Chem Biol. 1998 Sep; 5(9):461-73. PMID: 9751642.
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    217. Schuller DJ, Wilks A, Ortiz de Montellano P, Poulos TL. Crystallization of recombinant human heme oxygenase-1. Protein Sci. 1998 Aug; 7(8):1836-8. PMID: 10082382.
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    218. Vidakovic M, Sligar SG, Li H, Poulos TL. Understanding the role of the essential Asp251 in cytochrome p450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect. Biochemistry. 1998 Jun 30; 37(26):9211-9. PMID: 9649301.
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    219. Nissum M, Neri F, Mandelman D, Poulos TL, Smulevich G. Spectroscopic characterization of recombinant pea cytosolic ascorbate peroxidase: similarities and differences with cytochrome c peroxidase. Biochemistry. 1998 Jun 02; 37(22):8080-7. PMID: 9609702.
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    220. Poulos TL, Raman CS, Li H. NO news is good news. Structure. 1998 Mar 15; 6(3):255-8. PMID: 9551547.
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    221. Gajhede M, Schuller DJ, Henriksen A, Smith AT, Poulos TL. Crystal structure of horseradish peroxidase C at 2.15 A resolution. Nat Struct Biol. 1997 Dec; 4(12):1032-8. PMID: 9406554.
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    222. Daff SN, Chapman SK, Turner KL, Holt RA, Govindaraj S, Poulos TL, Munro AW. Redox control of the catalytic cycle of flavocytochrome P-450 BM3. Biochemistry. 1997 Nov 11; 36(45):13816-23. PMID: 9374858.
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    223. Sundaramoorthy M, Kishi K, Gold MH, Poulos TL. Crystal structures of substrate binding site mutants of manganese peroxidase. J Biol Chem. 1997 Jul 11; 272(28):17574-80. PMID: 9211904.
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    224. Govindaraj S, Poulos TL. The domain architecture of cytochrome P450BM-3. J Biol Chem. 1997 Mar 21; 272(12):7915-21. PMID: 9065459.
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    225. Hazzard JT, Govindaraj S, Poulos TL, Tollin G. Electron transfer between the FMN and heme domains of cytochrome P450BM-3. Effects of substrate and CO. J Biol Chem. 1997 Mar 21; 272(12):7922-6. PMID: 9065460.
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    226. Li H, Poulos TL. The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid. Nat Struct Biol. 1997 Feb; 4(2):140-6. PMID: 9033595.
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    227. Velazquez P, Cribbs DH, Poulos TL, Tenner AJ. Aspartate residue 7 in amyloid beta-protein is critical for classical complement pathway activation: implications for Alzheimer's disease pathogenesis. Nat Med. 1997 Jan; 3(1):77-9. PMID: 8986745.
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    228. Cupp-Vickery JR, Poulos TL. Structure of cytochrome P450eryF: substrate, inhibitors, and model compounds bound in the active site. Steroids. 1997 Jan; 62(1):112-6. PMID: 9029724.
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    229. Govindaraj S, Poulos TL. Probing the structure of the linker connecting the reductase and heme domains of cytochrome P450BM-3 using site-directed mutagenesis. Protein Sci. 1996 Jul; 5(7):1389-93. PMID: 8819171.
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    230. Cupp-Vickery JR, Han O, Hutchinson CR, Poulos TL. Substrate-assisted catalysis in cytochrome P450eryF. Nat Struct Biol. 1996 Jul; 3(7):632-7. PMID: 8673608.
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    231. Bonagura CA, Sundaramoorthy M, Pappa HS, Patterson WR, Poulos TL. An engineered cation site in cytochrome c peroxidase alters the reactivity of the redox active tryptophan. Biochemistry. 1996 May 14; 35(19):6107-15. PMID: 8634253.
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    232. Poulos TL. Ligands and electrons and haem proteins. Nat Struct Biol. 1996 May; 3(5):401-3. PMID: 8612066.
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    233. Pappa H, Li H, Sundaramoorthy M, Arciero D, Hooper A, Poulos TL. Crystallization and preliminary crystallographic analysis of cytochrome c553 peroxidase from Nitrosomonas europaea. J Struct Biol. 1996 May-Jun; 116(3):429-31. PMID: 8813001.
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    234. Pappa HS, Tajbaksh S, Saunders AJ, Pielak GJ, Poulos TL. Probing the cytochrome c peroxidase-cytochrome c electron transfer reaction using site specific cross-linking. Biochemistry. 1996 Apr 16; 35(15):4837-45. PMID: 8664274.
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    235. Schuller DJ, Ban N, Huystee RB, McPherson A, Poulos TL. The crystal structure of peanut peroxidase. Structure. 1996 Mar 15; 4(3):311-21. PMID: 8805539.
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    236. Li H, Poulos TL. Conformational dynamics in cytochrome P450-substrate interactions. Biochimie. 1996; 78(8-9):695-9. PMID: 9010597.
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    237. Poulos TL. Approaches to crystallizing P450s. Methods Enzymol. 1996; 272:358-68. PMID: 8791795.
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    238. Sundaramoorthy M, Terner J, Poulos TL. The crystal structure of chloroperoxidase: a heme peroxidase--cytochrome P450 functional hybrid. Structure. 1995 Dec 15; 3(12):1367-77. PMID: 8747463.
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    239. Poulos TL. Cytochrome P450. Curr Opin Struct Biol. 1995 Dec; 5(6):767-74. PMID: 8749364.
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    240. Alam SL, Satterlee JD, Mauro JM, Poulos TL, Erman JE. Proton NMR studies of cytochrome c peroxidase mutant N82A: hyperfine resonance assignments, identification of two interconverting enzyme ofecies, quantitating the rate of interconversion, and determination of equilibrium constants. Biochemistry. 1995 Nov 28; 34(47):15496-503. PMID: 7492551.
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    241. Yeom H, Sligar SG, Li H, Poulos TL, Fulco AJ. The role of Thr268 in oxygen activation of cytochrome P450BM-3. Biochemistry. 1995 Nov 14; 34(45):14733-40. PMID: 7578081.
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    242. Smulevich G, Neri F, Willemsen O, Choudhury K, Marzocchi MP, Poulos TL. Effect of the His175-->Glu mutation on the heme pocket architecture of cytochrome c peroxidase. Biochemistry. 1995 Oct 17; 34(41):13485-90. PMID: 7577937.
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    243. Govindaraj S, Poulos TL. Role of the linker region connecting the reductase and heme domains in cytochrome P450BM-3. Biochemistry. 1995 Sep 05; 34(35):11221-6. PMID: 7669780.
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    244. Sundaramoorthy M, Mauro JM, Sullivan AM, Terner J, Poulos TL. Preliminary crystallographic analysis of chloroperoxidase from Caldariomyces fumago. Acta Crystallogr D Biol Crystallogr. 1995 Sep 01; 51(Pt 5):842-4. PMID: 15299822.
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    245. Pappa HS, Poulos TL. Site-specific cross-linking as a method for studying intramolecular electron transfer. Biochemistry. 1995 May 23; 34(20):6573-80. PMID: 7756288.
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    246. Poulos TL, Patterson WR, Sundaramoorthy M. The crystal structure of ascorbate and manganese peroxidases: the role of non-haem metal in the catalytic mechanism. Biochem Soc Trans. 1995 May; 23(2):228-32. PMID: 7672248.
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    247. Patterson WR, Poulos TL. Crystal structure of recombinant pea cytosolic ascorbate peroxidase. Biochemistry. 1995 Apr 04; 34(13):4331-41. PMID: 7703247.
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    248. Patterson WR, Poulos TL, Goodin DB. Identification of a porphyrin pi cation radical in ascorbate peroxidase compound I. Biochemistry. 1995 Apr 04; 34(13):4342-5. PMID: 7703248.
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    249. Cupp-Vickery JR, Poulos TL. Structure of cytochrome P450eryF involved in erythromycin biosynthesis. Nat Struct Biol. 1995 Feb; 2(2):144-53. PMID: 7749919.
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    250. Li H, Poulos TL. Modeling protein-substrate interactions in the heme domain of cytochrome P450(BM-3). Acta Crystallogr D Biol Crystallogr. 1995 Jan 01; 51(Pt 1):21-32. PMID: 15299332.
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    251. Sundaramoorthy M, Kishi K, Gold MH, Poulos TL. The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution. J Biol Chem. 1994 Dec 30; 269(52):32759-67. PMID: 7806497.
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    252. Cupp-Vickery JR, Li H, Poulos TL. Preliminary crystallographic analysis of an enzyme involved in erythromycin biosynthesis: cytochrome P450eryF. Proteins. 1994 Oct; 20(2):197-201. PMID: 7846029.
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    253. Gonvindaraj S, Li H, Poulos TL. Flavin supported fatty acid oxidation by the heme domain of Bacillus megaterium cytochrome P450BM-3. Biochem Biophys Res Commun. 1994 Sep 30; 203(3):1745-9. PMID: 7945324.
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    254. DeLauder SF, Mauro JM, Poulos TL, Williams JC, Schwarz FP. Thermodynamics of hydrogen cyanide and hydrogen fluoride binding to cytochrome c peroxidase and its Asn-82-->Asp mutant. Biochem J. 1994 Sep 01; 302 ( Pt 2):437-42. PMID: 8092995.
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    255. Satterlee JD, Alam SL, Mauro JM, Erman JE, Poulos TL. The effect of the Asn82-->Asp mutation in yeast cytochrome c peroxidase studied by proton NMR spectroscopy. Eur J Biochem. 1994 Aug 15; 224(1):81-7. PMID: 8076654.
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    256. Choudhury K, Sundaramoorthy M, Hickman A, Yonetani T, Woehl E, Dunn MF, Poulos TL. Role of the proximal ligand in peroxidase catalysis. Crystallographic, kinetic, and spectral studies of cytochrome c peroxidase proximal ligand mutants. J Biol Chem. 1994 Aug 12; 269(32):20239-49. PMID: 8051115.
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    257. Patterson WR, Poulos TL. Characterization and crystallization of recombinant pea cytosolic ascorbate peroxidase. J Biol Chem. 1994 Jun 24; 269(25):17020-4. PMID: 8006006.
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    258. Li H, Poulos TL. Structural variation in heme enzymes: a comparative analysis of peroxidase and P450 crystal structures. Structure. 1994 Jun 15; 2(6):461-4. PMID: 7922023.
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    259. Sundaramoorthy M, Kishi K, Gold MH, Poulos TL. Preliminary crystallographic analysis of manganese peroxidase from Phanerochaete chrysosporium. J Mol Biol. 1994 May 20; 238(5):845-8. PMID: 8182752.
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    260. Poulos TL. Peroxidases. Curr Opin Biotechnol. 1993 Aug; 4(4):484-9. PMID: 7764068.
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    261. Raag R, Li H, Jones BC, Poulos TL. Inhibitor-induced conformational change in cytochrome P-450CAM. Biochemistry. 1993 May 04; 32(17):4571-8. PMID: 8485133.
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    262. Poulos TL, Edwards SL, Wariishi H, Gold MH. Crystallographic refinement of lignin peroxidase at 2 A. J Biol Chem. 1993 Feb 25; 268(6):4429-40. PMID: 8440725.
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    263. Edwards SL, Raag R, Wariishi H, Gold MH, Poulos TL. Crystal structure of lignin peroxidase. Proc Natl Acad Sci U S A. 1993 Jan 15; 90(2):750-4. PMID: 11607355.
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    264. Choudhury K, Sundaramoorthy M, Mauro JM, Poulos TL. Conversion of the proximal histidine ligand to glutamine restores activity to an inactive mutant of cytochrome c peroxidase. J Biol Chem. 1992 Dec 25; 267(36):25656-9. PMID: 1334481.
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    265. Zhou DJ, Korzekwa KR, Poulos T, Chen SA. A site-directed mutagenesis study of human placental aromatase. J Biol Chem. 1992 Jan 15; 267(2):762-8. PMID: 1730667.
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    266. Poulos TL, Raag R. Cytochrome P450cam: crystallography, oxygen activation, and electron transfer. FASEB J. 1992 Jan 06; 6(2):674-9. PMID: 1537455.
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    267. Veerapandian B, Gilliland GL, Raag R, Svensson AL, Masui Y, Hirai Y, Poulos TL. Functional implications of interleukin-1 beta based on the three-dimensional structure. Proteins. 1992 Jan; 12(1):10-23. PMID: 1553379.
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    268. Raag R, Martinis SA, Sligar SG, Poulos TL. Crystal structure of the cytochrome P-450CAM active site mutant Thr252Ala. Biochemistry. 1991 Dec 03; 30(48):11420-9. PMID: 1742281.
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    269. Darwish K, Li HY, Poulos TL. Engineering proteins, subcloning and hyperexpressing oxidoreductase genes. Protein Eng. 1991 Aug; 4(6):701-8. PMID: 1658778.
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    270. Li HY, Darwish K, Poulos TL. Characterization of recombinant Bacillus megaterium cytochrome P-450 BM-3 and its two functional domains. J Biol Chem. 1991 Jun 25; 266(18):11909-14. PMID: 1904873.
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    271. Raag R, Poulos TL. Crystal structures of cytochrome P-450CAM complexed with camphane, thiocamphor, and adamantane: factors controlling P-450 substrate hydroxylation. Biochemistry. 1991 Mar 12; 30(10):2674-84. PMID: 2001355.
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    272. Poulos TL. Modeling of mammalian P450s on basis of P450cam X-ray structure. Methods Enzymol. 1991; 206:11-30. PMID: 1784202.
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    273. Whitlow M, Howard AJ, Finzel BC, Poulos TL, Winborne E, Gilliland GL. A metal-mediated hydride shift mechanism for xylose isomerase based on the 1.6 A Streptomyces rubiginosus structures with xylitol and D-xylose. Proteins. 1991; 9(3):153-73. PMID: 2006134.
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    274. Raag R, Swanson BA, Poulos TL, Ortiz de Montellano PR. Formation, crystal structure, and rearrangement of a cytochrome P-450cam iron-phenyl complex. Biochemistry. 1990 Sep 04; 29(35):8119-26. PMID: 2261467.
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    275. Smulevich G, Wang Y, Edwards SL, Poulos TL, English AM, Spiro TG. Resonance Raman spectroscopy of cytochrome c peroxidase single crystals on a variable-temperature microscope stage. Biochemistry. 1990 Mar 13; 29(10):2586-92. PMID: 2159325.
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    276. Edwards SL, Poulos TL. Ligand binding and structural perturbations in cytochrome c peroxidase. A crystallographic study. J Biol Chem. 1990 Feb 15; 265(5):2588-95. PMID: 2154451.
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    277. Stayton PS, Poulos TL, Sligar SG. Putidaredoxin competitively inhibits cytochrome b5-cytochrome P-450cam association: a proposed molecular model for a cytochrome P-450cam electron-transfer complex. Biochemistry. 1989 Oct 03; 28(20):8201-5. PMID: 2690937.
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    278. Raag R, Poulos TL. Crystal structure of the carbon monoxide-substrate-cytochrome P-450CAM ternary complex. Biochemistry. 1989 Sep 19; 28(19):7586-92. PMID: 2611203.
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    279. Furuya H, Shimizu T, Hirano K, Hatano M, Fujii-Kuriyama Y, Raag R, Poulos TL. Site-directed mutageneses of rat liver cytochrome P-450d: catalytic activities toward benzphetamine and 7-ethoxycoumarin. Biochemistry. 1989 Aug 22; 28(17):6848-57. PMID: 2819037.
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    280. Poulos TL. Reversing enzyme specificity. Nature. 1989 Jun 22; 339(6226):580-1. PMID: 2733788.
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    281. Raag R, Poulos TL. The structural basis for substrate-induced changes in redox potential and spin equilibrium in cytochrome P-450CAM. Biochemistry. 1989 Jan 24; 28(2):917-22. PMID: 2713354.
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    282. Pantoliano MW, Whitlow M, Wood JF, Rollence ML, Finzel BC, Gilliland GL, Poulos TL, Bryan PN. The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test case. Biochemistry. 1988 Nov 01; 27(22):8311-7. PMID: 3072018.
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    283. Edwards SL, Kraut J, Poulos TL. Crystal structure of nitric oxide inhibited cytochrome c peroxidase. Biochemistry. 1988 Oct 18; 27(21):8074-81. PMID: 2852951.
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    284. Poulos TL. Cytochrome P450: molecular architecture, mechanism, and prospects for rational inhibitor design. Pharm Res. 1988 Feb; 5(2):67-75. PMID: 3073382.
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    285. Poulos TL. Heme enzyme crystal structures. Adv Inorg Biochem. 1988; 7:1-36. PMID: 2821744.
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    286. Poulos TL, Howard AJ. Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam. Biochemistry. 1987 Dec 15; 26(25):8165-74. PMID: 3442650.
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    287. Poulos TL, Sheriff S, Howard AJ. Cocrystals of yeast cytochrome c peroxidase and horse heart cytochrome c. J Biol Chem. 1987 Oct 15; 262(29):13881-4. PMID: 2820983.
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    288. Poulos TL, Finzel BC, Howard AJ. High-resolution crystal structure of cytochrome P450cam. J Mol Biol. 1987 Jun 05; 195(3):687-700. PMID: 3656428.
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    289. Hazzard JT, Poulos TL, Tollin G. Kinetics of reduction by free flavin semiquinones of the components of the cytochrome c-cytochrome c peroxidase complex and intracomplex electron transfer. Biochemistry. 1987 May 19; 26(10):2836-48. PMID: 3038167.
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    290. Pantoliano MW, Ladner RC, Bryan PN, Rollence ML, Wood JF, Poulos TL. Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond. Biochemistry. 1987 Apr 21; 26(8):2077-82. PMID: 3476160.
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    291. Gilliland GL, Howard AJ, Winborne EL, Poulos TL, Stewart DB, Durham DR. Crystallization and preliminary x-ray diffraction studies of subtilisin GX from Bacillus sp. GX6644. J Biol Chem. 1987 Mar 25; 262(9):4280-3. PMID: 3549729.
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    292. Bryan PN, Rollence ML, Pantoliano MW, Wood J, Finzel BC, Gilliland GL, Howard AJ, Poulos TL. Proteases of enhanced stability: characterization of a thermostable variant of subtilisin. Proteins. 1986 Dec; 1(4):326-34. PMID: 3329733.
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    293. Poulos TL, Finzel BC, Howard AJ. Crystal structure of substrate-free Pseudomonas putida cytochrome P-450. Biochemistry. 1986 Sep 09; 25(18):5314-22. PMID: 3768350.
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    294. Bryan P, Pantoliano MW, Quill SG, Hsiao HY, Poulos T. Site-directed mutagenesis and the role of the oxyanion hole in subtilisin. Proc Natl Acad Sci U S A. 1986 Jun; 83(11):3743-5. PMID: 3520553.
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    295. Chance M, Powers L, Poulos T, Chance B. Cytochrome c peroxidase compound ES is identical with horseradish peroxide compound I in iron-ligand distances. Biochemistry. 1986 Mar 25; 25(6):1266-70. PMID: 3008825.
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    296. Poulos TL, Finzel BC, Gunsalus IC, Wagner GC, Kraut J. The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450. J Biol Chem. 1985 Dec 25; 260(30):16122-30. PMID: 4066706.
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    297. Evangelista-Kirkup R, Crisanti M, Poulos TL, Spiro TG. Resonance Raman spectroscopy shows different temperature-dependent coordination equilibria for native horseradish and cytochrome c peroxidase. FEBS Lett. 1985 Oct 14; 190(2):221-6. PMID: 2995134.
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    298. Chance B, Powers L, Ching Y, Poulos T, Schonbaum GR, Yamazaki I, Paul KG. X-ray absorption studies of intermediates in peroxidase activity. Arch Biochem Biophys. 1984 Dec; 235(2):596-611. PMID: 6097192.
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    299. Edwards SL, Poulos TL, Kraut J. The crystal structure of fluoride-inhibited cytochrome c peroxidase. J Biol Chem. 1984 Nov 10; 259(21):12984-8. PMID: 6092357.
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    302. Poulos TL, Mauk AG. Models for the complexes formed between cytochrome b5 and the subunits of methemoglobin. J Biol Chem. 1983 Jun 25; 258(12):7369-73. PMID: 6863249.
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    303. Poulos TL, Perez M, Wagner GC. Preliminary crystallographic data on cytochrome P-450CAM. J Biol Chem. 1982 Sep 10; 257(17):10427-9. PMID: 7107613.
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    304. Waldmeyer B, Bechtold R, Bosshard HR, Poulos TL. The cytochrome c peroxidase.cytochrome c electron transfer complex. Experimental support of a hypothetical model. J Biol Chem. 1982 Jun 10; 257(11):6073-6. PMID: 6281255.
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    305. Poulos TL, Kraut J. A hypothetical model of the cytochrome c peroxidase . cytochrome c electron transfer complex. J Biol Chem. 1980 Nov 10; 255(21):10322-30. PMID: 6253470.
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    306. Poulos TL, Kraut J. The stereochemistry of peroxidase catalysis. J Biol Chem. 1980 Sep 10; 255(17):8199-205. PMID: 6251047.
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    308. Poulos TL, Freer ST, Alden RA, Xuong NH, Edwards SL, Hamlin RC, Kraut J. Crystallographic determination of the heme orientation and location of the cyanide binding site in yeast cytochrome c peroxidase. J Biol Chem. 1978 May 25; 253(10):3730-5. PMID: 206550.
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    309. Poulos TL, Alden RA, Freer ST, Birktoft JJ, Kraut J. Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. X-ray crystallographic comparison of lysine- and phenylalanine-polypeptide chloromethyl ketone-inhibited subtilisin. J Biol Chem. 1976 Feb 25; 251(4):1097-103. PMID: 1249069.
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    310. Poulos TL, Price PA. The involvement of serine and carboxyl groups in the activity of bovine pancreatic deoxyribonuclease A. J Biol Chem. 1974 Mar 10; 249(5):1453-7. PMID: 4361736.
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    311. Poulos TL, Price PA. Some effects of calcium ions on the structure of bovine pancreatic deoxyribonuclease A. J Biol Chem. 1972 May 10; 247(9):2900-4. PMID: 5063468.
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    312. Poulos TL, Price PA. The identification of a tryptophan residue essential to the catalytic activity of bovine pancreatic deoxyribonuclease. J Biol Chem. 1971 Jun 25; 246(12):4041-5. PMID: 5105445.
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