Loading...

Poulos, Thomas

TitleChancellor's Professor
InstitutionUniversity of California, Irvine
DepartmentMolecular Biology & Biochemistry
Address2206 Natural Sciences 1
Zot 3900
Irvine CA 92697
Phone(949) 824-7020, 4322, 2856
vCardDownload vCard

    Collapse Research 
    Collapse Research Activities and Funding
    Training Program in Chemical and Structural Biology
    NIH/NIGMS T32GM108561Jul 1, 2014 - Jun 30, 2019
    Role: Principal Investigator
    Acquisition of a Bruker X8 Prospector Protein X-ray Crystallography System
    NIH/NCRR S10RR024679May 7, 2009 - May 6, 2010
    Role: Principal Investigator
    Nitroxyl adducts as structural probes of oxygenase/substrate interactions
    NIH/NIEHS R21ES016441Jan 1, 2008 - Dec 31, 2009
    Role: Principal Investigator
    Structural Studies on Nitric Oxide Synthase
    NIH/NIGMS R01GM057353May 1, 1998 - Aug 31, 2019
    Role: Principal Investigator
    7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEIN
    NIH/NIGMS R01GM045209Jan 1, 1991 - Dec 31, 2003
    Role: Principal Investigator
    NITROGENASE MECHANISM AND ASSEMBLY
    NIH/NIGMS R01GM043144Dec 1, 1989 - Mar 31, 2004
    Role: Principal Investigator
    Diversity in Heme Protein Structure and Function
    NIH/NIGMS R01GM042614Jul 1, 1989 - Mar 31, 2017
    Role: Principal Investigator
    Structural Studies on Cytochromes P450 and Related Proteins
    NIH/NIGMS R01GM033688Mar 1, 1985 - Nov 30, 2014
    Role: Principal Investigator
    X-RAY STUDIES OF PROTEIN STRUCTURE
    NIH/NIGMS R01GM033325Aug 1, 1983 - Jul 31, 1986
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Follmer AH, Mahomed M, Goodin DB, Poulos TL. Substrate Dependent Allosteric Regulation in Cytochrome P450cam (CYP101A1). J Am Chem Soc. 2018 Oct 30. PMID: 30376314.
      View in: PubMed
    2. Li H, Evenson RJ, Chreifi G, Silverman RB, Poulos TL. Structural Basis for Isoform Selective Nitric Oxide Synthase Inhibition by Thiophene-2-carboximidamides. Biochemistry. 2018 Oct 24. PMID: 30335983.
      View in: PubMed
    3. Lewis-Ballester A, Karkashon S, Batabyal D, Poulos TL, Yeh SR. Inhibition Mechanisms of Human Indoleamine 2,3 Dioxygenase 1. J Am Chem Soc. 2018 Jun 13. PMID: 29897749.
      View in: PubMed
    4. Tripathi S, Poulos TL. Testing the N-Terminal Velcro Model of CooA Carbon Monoxide Activation. Biochemistry. 2018 May 11. PMID: 29708736.
      View in: PubMed
    5. Batabyal D, Poulos TL. Effect of redox partner binding on CYP101D1 conformational dynamics. J Inorg Biochem. 2018 Jun; 183:179-183. PMID: 29550100.
      View in: PubMed
    6. Lewis-Ballester A, Pham KN, Batabyal D, Karkashon S, Bonanno JB, Poulos TL, Yeh SR. Structural insights into substrate and inhibitor binding sites in human indoleamine 2,3-dioxygenase 1. Nat Commun. 2017 Nov 22; 8(1):1693. PMID: 29167421.
      View in: PubMed
    7. Do HT, Wang HY, Li H, Chreifi G, Poulos TL, Silverman RB. Improvement of Cell Permeability of Human Neuronal Nitric Oxide Synthase Inhibitors Using Potent and Selective 2-Aminopyridine-Based Scaffolds with a Fluorobenzene Linker. J Med Chem. 2017 Nov 22; 60(22):9360-9375. PMID: 29091437.
      View in: PubMed
    8. Benabbas A, Sun Y, Poulos TL, Champion PM. Ultrafast CO Kinetics in Heme Proteins: Adiabatic Ligand Binding and Heavy Atom Tunneling. J Am Chem Soc. 2017 11 08; 139(44):15738-15747. PMID: 28984134.
      View in: PubMed
    9. Guo Z, Sevrioukova IF, Denisov IG, Zhang X, Chiu TL, Thomas DG, Hanse EA, Cuellar RAD, Grinkova YV, Langenfeld VW, Swedien DS, Stamschror JD, Alvarez J, Luna F, Galván A, Bae YK, Wulfkuhle JD, Gallagher RI, Petricoin EF, Norris B, Flory CM, Schumacher RJ, O'Sullivan MG, Cao Q, Chu H, Lipscomb JD, Atkins WM, Gupta K, Kelekar A, Blair IA, Capdevila JH, Falck JR, Sligar SG, Poulos TL, Georg GI, Ambrose E, Potter DA. Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria. Cell Chem Biol. 2017 Oct 19; 24(10):1314. PMID: 29053951.
      View in: PubMed
    10. Guo Z, Sevrioukova IF, Denisov IG, Zhang X, Chiu TL, Thomas DG, Hanse EA, Cuellar RAD, Grinkova YV, Langenfeld VW, Swedien DS, Stamschror JD, Alvarez J, Luna F, Galván A, Bae YK, Wulfkuhle JD, Gallagher RI, Petricoin EF, Norris B, Flory CM, Schumacher RJ, O'Sullivan MG, Cao Q, Chu H, Lipscomb JD, Atkins WM, Gupta K, Kelekar A, Blair IA, Capdevila JH, Falck JR, Sligar SG, Poulos TL, Georg GI, Ambrose E, Potter DA. Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria. Cell Chem Biol. 2017 Oct 19; 24(10):1259-1275.e6. PMID: 28919040.
      View in: PubMed
    11. Hollingsworth SA, Nguyen BD, Chreifi G, Arce AP, Poulos TL. Insights into the Dynamics and Dissociation Mechanism of a Protein Redox Complex Using Molecular Dynamics. J Chem Inf Model. 2017 09 25; 57(9):2344-2350. PMID: 28841378.
      View in: PubMed
    12. Batabyal D, Richards LS, Poulos TL. Effect of Redox Partner Binding on Cytochrome P450 Conformational Dynamics. J Am Chem Soc. 2017 09 20; 139(37):13193-13199. PMID: 28823160.
      View in: PubMed
    13. Pensa AV, Cinelli MA, Li H, Chreifi G, Mukherjee P, Roman LJ, Martásek P, Poulos TL, Silverman RB. Hydrophilic, Potent, and Selective 7-Substituted 2-Aminoquinolines as Improved Human Neuronal Nitric Oxide Synthase Inhibitors. J Med Chem. 2017 Aug 24; 60(16):7146-7165. PMID: 28776992.
      View in: PubMed
    14. Meneghini LM, Tripathi S, Woodworth MA, Majumdar S, Poulos TL, Weiss GA. Dissecting binding of a ß-barrel membrane protein by phage display. Mol Biosyst. 2017 Jul 25; 13(8):1438-1447. PMID: 28627567.
      View in: PubMed
    15. Chreifi G, Dejam D, Poulos TL. Crystal structure and functional analysis of Leishmania major pseudoperoxidase. J Biol Inorg Chem. 2017 Aug; 22(6):919-927. PMID: 28584975.
      View in: PubMed
    16. Cinelli MA, Li H, Chreifi G, Poulos TL, Silverman RB. Nitrile in the Hole: Discovery of a Small Auxiliary Pocket in Neuronal Nitric Oxide Synthase Leading to the Development of Potent and Selective 2-Aminoquinoline Inhibitors. J Med Chem. 2017 May 11; 60(9):3958-3978. PMID: 28422508.
      View in: PubMed
    17. Sevrioukova IF, Poulos TL. Structural basis for regiospecific midazolam oxidation by human cytochrome P450 3A4. Proc Natl Acad Sci U S A. 2017 01 17; 114(3):486-491. PMID: 28031486.
      View in: PubMed
    18. Poulos TL, Li H. Nitric oxide synthase and structure-based inhibitor design. Nitric Oxide. 2017 Feb 28; 63:68-77. PMID: 27890696.
      View in: PubMed
    19. Batabyal D, Lewis-Ballester A, Yeh SR, Poulos TL. A Comparative Analysis of the Effector Role of Redox Partner Binding in Bacterial P450s. Biochemistry. 2016 Nov 29; 55(47):6517-6523. PMID: 27808504.
      View in: PubMed
    20. Holden JK, Lewis MC, Cinelli MA, Abdullatif Z, Pensa AV, Silverman RB, Poulos TL. Targeting Bacterial Nitric Oxide Synthase with Aminoquinoline-Based Inhibitors. Biochemistry. 2016 Oct 04; 55(39):5587-5594. PMID: 27607918.
      View in: PubMed
    21. Hollingsworth SA, Batabyal D, Nguyen BD, Poulos TL. Conformational selectivity in cytochrome P450 redox partner interactions. Proc Natl Acad Sci U S A. 2016 08 02; 113(31):8723-8. PMID: 27439869; PMCID: PMC4978276 [Available on 02/02/17].
    22. Li H, Wang HY, Kang S, Silverman RB, Poulos TL. Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase. Biochemistry. 2016 07 05; 55(26):3702-7. PMID: 27250740.
      View in: PubMed
    23. Wang HY, Qin Y, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibition by Optimization of the 2-Aminopyridine-Based Scaffold with a Pyridine Linker. J Med Chem. 2016 05 26; 59(10):4913-25. PMID: 27050842; PMCID: PMC4882237 [Available on 05/26/17].
    24. Cinelli MA, Li H, Pensa AV, Kang S, Roman LJ, Martásek P, Poulos TL, Silverman RB. Correction to Phenyl Ether- and Aniline-Containing 2-Aminoquinolines as Potent and Selective Inhibitors of Neuronal Nitric Oxide Synthase. J Med Chem. 2016 Feb 11; 59(3):1246. PMID: 26807481; PMCID: PMC4957696.
    25. Chreifi G, Baxter EL, Doukov T, Cohen AE, McPhillips SE, Song J, Meharenna YT, Soltis SM, Poulos TL. Crystal structure of the pristine peroxidase ferryl center and its relevance to proton-coupled electron transfer. Proc Natl Acad Sci U S A. 2016 Feb 02; 113(5):1226-31. PMID: 26787871; PMCID: PMC4747753.
    26. Fields JB, Hollingsworth SA, Chreifi G, Heyden M, Arce AP, Magaña-Garcia HI, Poulos TL, Tobias DJ. "Bind and Crawl" Association Mechanism of Leishmania major Peroxidase and Cytochrome c Revealed by Brownian and Molecular Dynamics Simulations. Biochemistry. 2015 Dec 15; 54(49):7272-82. PMID: 26598276.
      View in: PubMed
    27. Cinelli MA, Li H, Pensa AV, Kang S, Roman LJ, Martásek P, Poulos TL, Silverman RB. Phenyl Ether- and Aniline-Containing 2-Aminoquinolines as Potent and Selective Inhibitors of Neuronal Nitric Oxide Synthase. J Med Chem. 2015 Nov 12; 58(21):8694-712. PMID: 26469213; PMCID: PMC4644118 [Available on 11/12/16].
    28. Hollingsworth SA, Holden JK, Li H, Poulos TL. Elucidating nitric oxide synthase domain interactions by molecular dynamics. Protein Sci. 2016 Feb; 25(2):374-82. PMID: 26448477; PMCID: PMC4815339.
    29. Kaur P, Chamberlin AR, Poulos TL, Sevrioukova IF. Structure-Based Inhibitor Design for Evaluation of a CYP3A4 Pharmacophore Model. J Med Chem. 2016 05 12; 59(9):4210-20. PMID: 26371436; PMCID: PMC4998966.
    30. Kang S, Li H, Tang W, Martásek P, Roman LJ, Poulos TL, Silverman RB. 2-Aminopyridines with a Truncated Side Chain To Improve Human Neuronal Nitric Oxide Synthase Inhibitory Potency and Selectivity. J Med Chem. 2015 Jul 23; 58(14):5548-60. PMID: 26120733; PMCID: PMC4514563.
    31. Holden JK, Dejam D, Lewis MC, Huang H, Kang S, Jing Q, Xue F, Silverman RB, Poulos TL. Inhibitor Bound Crystal Structures of Bacterial Nitric Oxide Synthase. Biochemistry. 2015 Jul 07; 54(26):4075-82. PMID: 26062720; PMCID: PMC4860011.
    32. Sevrioukova IF, Poulos TL. Anion-Dependent Stimulation of CYP3A4 Monooxygenase. Biochemistry. 2015 Jul 07; 54(26):4083-96. PMID: 26066995.
      View in: PubMed
    33. Holden JK, Kang S, Beasley FC, Cinelli MA, Li H, Roy SG, Dejam D, Edinger AL, Nizet V, Silverman RB, Poulos TL. Nitric Oxide Synthase as a Target for Methicillin-Resistant Staphylococcus aureus. Chem Biol. 2015 Jun 18; 22(6):785-92. PMID: 26091171; PMCID: PMC4475277.
    34. Astashkin AV, Chen L, Elmore BO, Kunwar D, Miao Y, Li H, Poulos TL, Roman LJ, Feng C. Probing the Hydrogen Bonding of the Ferrous-NO Heme Center of nNOS by Pulsed Electron Paramagnetic Resonance. J Phys Chem A. 2015 Jun 25; 119(25):6641-9. PMID: 26035438; PMCID: PMC4533915.
    35. Chreifi G, Hollingsworth SA, Li H, Tripathi S, Arce AP, Magaña-Garcia HI, Poulos TL. Enzymatic Mechanism of Leishmania major Peroxidase and the Critical Role of Specific Ionic Interactions. Biochemistry. 2015 Jun 02; 54(21):3328-36. PMID: 25941976; PMCID: PMC4533909.
    36. Tang W, Li H, Doud EH, Chen Y, Choing S, Plaza C, Kelleher NL, Poulos TL, Silverman RB. Mechanism of Inactivation of Neuronal Nitric Oxide Synthase by (S)-2-Amino-5-(2-(methylthio)acetimidamido)pentanoic Acid. J Am Chem Soc. 2015 May 13; 137(18):5980-9. PMID: 25874809; PMCID: PMC4431946.
    37. Tang W, Li H, Poulos TL, Silverman RB. Mechanistic studies of inactivation of inducible nitric oxide synthase by amidines. Biochemistry. 2015 Apr 21; 54(15):2530-8. PMID: 25811913; PMCID: PMC4410366.
    38. Kaluka D, Batabyal D, Chiang BY, Poulos TL, Yeh SR. Spectroscopic and mutagenesis studies of human PGRMC1. Biochemistry. 2015 Mar 03; 54(8):1638-47. PMID: 25675345; PMCID: PMC4533898.
    39. Holden JK, Kang S, Hollingsworth SA, Li H, Lim N, Chen S, Huang H, Xue F, Tang W, Silverman RB, Poulos TL. Structure-based design of bacterial nitric oxide synthase inhibitors. J Med Chem. 2015 Jan 22; 58(2):994-1004. PMID: 25522110.
      View in: PubMed
    40. Sevrioukova IF, Poulos TL. Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions. Adv Exp Med Biol. 2015; 851:83-105. PMID: 26002732; PMCID: PMC4532289.
    41. Mukherjee P, Li H, Sevrioukova I, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Novel 2,4-disubstituted pyrimidines as potent, selective, and cell-permeable inhibitors of neuronal nitric oxide synthase. J Med Chem. 2015 Feb 12; 58(3):1067-88. PMID: 25489882; PMCID: PMC4329833.
    42. Hollingsworth SA, Poulos TL. Molecular dynamics of the P450cam-Pdx complex reveals complex stability and novel interface contacts. Protein Sci. 2015 Jan; 24(1):49-57. PMID: 25307478; PMCID: PMC4282411.
    43. Li H, Jamal J, Plaza C, Pineda SH, Chreifi G, Jing Q, Cinelli MA, Silverman RB, Poulos TL. Structures of human constitutive nitric oxide synthases. Acta Crystallogr D Biol Crystallogr. 2014 Oct; 70(Pt 10):2667-74. PMID: 25286850; PMCID: PMC4188008.
    44. Holden JK, Lim N, Poulos TL. Identification of redox partners and development of a novel chimeric bacterial nitric oxide synthase for structure activity analyses. J Biol Chem. 2014 Oct 17; 289(42):29437-45. PMID: 25194416; PMCID: PMC4200291.
    45. Jing Q, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Combination of chiral linkers with thiophenecarboximidamide heads to improve the selectivity of inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem Lett. 2014 Sep 15; 24(18):4504-4510. PMID: 25149509; PMCID: PMC4204799.
    46. Li H, Jamal J, Delker S, Plaza C, Ji H, Jing Q, Huang H, Kang S, Silverman RB, Poulos TL. The mobility of a conserved tyrosine residue controls isoform-dependent enzyme-inhibitor interactions in nitric oxide synthases. Biochemistry. 2014 Aug 19; 53(32):5272-9. PMID: 25089924; PMCID: PMC4139154.
    47. Astashkin AV, Chen L, Zhou X, Li H, Poulos TL, Liu KJ, Guillemette JG, Feng C. Pulsed electron paramagnetic resonance study of domain docking in neuronal nitric oxide synthase: the calmodulin and output state perspective. J Phys Chem A. 2014 Aug 28; 118(34):6864-72. PMID: 25046446; PMCID: PMC4148148.
    48. Chreifi G, Li H, McInnes CR, Gibson CL, Suckling CJ, Poulos TL. Communication between the zinc and tetrahydrobiopterin binding sites in nitric oxide synthase. Biochemistry. 2014 Jul 01; 53(25):4216-23. PMID: 24819538; PMCID: PMC4082377.
    49. Kang S, Tang W, Li H, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Nitric oxide synthase inhibitors that interact with both heme propionate and tetrahydrobiopterin show high isoform selectivity. J Med Chem. 2014 May 22; 57(10):4382-96. PMID: 24758147; PMCID: PMC4032192.
    50. Madrona Y, Hollingsworth SA, Tripathi S, Fields JB, Rwigema JC, Tobias DJ, Poulos TL. Crystal structure of cindoxin, the P450cin redox partner. Biochemistry. 2014 Mar 11; 53(9):1435-46. PMID: 24533927; PMCID: PMC3985796.
    51. Cinelli MA, Li H, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB. Simplified 2-aminoquinoline-based scaffold for potent and selective neuronal nitric oxide synthase inhibition. J Med Chem. 2014 Feb 27; 57(4):1513-30. PMID: 24472039; PMCID: PMC3954451.
    52. Huang H, Li H, Yang S, Chreifi G, Martásek P, Roman LJ, Meyskens FL, Poulos TL, Silverman RB. Potent and selective double-headed thiophene-2-carboximidamide inhibitors of neuronal nitric oxide synthase for the treatment of melanoma. J Med Chem. 2014 Feb 13; 57(3):686-700. PMID: 24447275; PMCID: PMC3983353.
    53. Jing Q, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. An Accessible Chiral Linker to Enhance Potency and Selectivity of Neuronal Nitric Oxide Synthase Inhibitors. ACS Med Chem Lett. 2014 Jan 09; 5(1):56-60. PMID: 24660051.
      View in: PubMed
    54. Poulos TL. Heme enzyme structure and function. Chem Rev. 2014 Apr 09; 114(7):3919-62. PMID: 24400737; PMCID: PMC3981943.
    55. Sevrioukova IF, Poulos TL. Ritonavir analogues as a probe for deciphering the cytochrome P450 3A4 inhibitory mechanism. Curr Top Med Chem. 2014; 14(11):1348-55. PMID: 24805065; PMCID: PMC4530633.
    56. Batabyal D, Poulos TL. Crystal structures and functional characterization of wild-type CYP101D1 and its active site mutants. Biochemistry. 2013 Dec 10; 52(49):8898-906. PMID: 24261604; PMCID: PMC3946301.
    57. Holden JK, Li H, Jing Q, Kang S, Richo J, Silverman RB, Poulos TL. Structural and biological studies on bacterial nitric oxide synthase inhibitors. Proc Natl Acad Sci U S A. 2013 Nov 05; 110(45):18127-31. PMID: 24145412; PMCID: PMC3831449.
    58. Jing Q, Li H, Chreifi G, Roman LJ, Martásek P, Poulos TL, Silverman RB. Chiral linkers to improve selectivity of double-headed neuronal nitric oxide synthase inhibitors. Bioorg Med Chem Lett. 2013 Oct 15; 23(20):5674-9. PMID: 23993333; PMCID: PMC3790268.
    59. Batabyal D, Li H, Poulos TL. Synergistic effects of mutations in cytochrome P450cam designed to mimic CYP101D1. Biochemistry. 2013 Aug 13; 52(32):5396-402. PMID: 23865948; PMCID: PMC3790332.
    60. Tripathi S, O'Neill MJ, Wilks A, Poulos TL. Crystal structure of the Pseudomonas aeruginosa cytoplasmic heme binding protein, Apo-PhuS. J Inorg Biochem. 2013 Nov; 128:131-6. PMID: 23973453; PMCID: PMC3843485.
    61. Madrona Y, Hollingsworth SA, Khan B, Poulos TL. P450cin active site water: implications for substrate binding and solvent accessibility. Biochemistry. 2013 Jul 30; 52(30):5039-50. PMID: 23829586; PMCID: PMC3790324.
    62. Sevrioukova IF, Poulos TL. Dissecting cytochrome P450 3A4-ligand interactions using ritonavir analogues. Biochemistry. 2013 Jul 02; 52(26):4474-81. PMID: 23746300.
      View in: PubMed
    63. Jing Q, Li H, Fang J, Roman LJ, Martásek P, Poulos TL, Silverman RB. In search of potent and selective inhibitors of neuronal nitric oxide synthase with more simple structures. Bioorg Med Chem. 2013 Sep 01; 21(17):5323-31. PMID: 23867386; PMCID: PMC3760172.
    64. Tripathi S, Li H, Poulos TL. Structural basis for effector control and redox partner recognition in cytochrome P450. Science. 2013 Jun 07; 340(6137):1227-30. PMID: 23744947.
      View in: PubMed
    65. Sevrioukova IF, Poulos TL. Pyridine-substituted desoxyritonavir is a more potent inhibitor of cytochrome P450 3A4 than ritonavir. J Med Chem. 2013 May 09; 56(9):3733-41. PMID: 23586711; PMCID: PMC4534002.
    66. Jansen Labby K, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. Methylated N(?)-hydroxy-L-arginine analogues as mechanistic probes for the second step of the nitric oxide synthase-catalyzed reaction. Biochemistry. 2013 May 07; 52(18):3062-73. PMID: 23586781; PMCID: PMC3678535.
    67. Huang H, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Structure-guided design of selective inhibitors of neuronal nitric oxide synthase. J Med Chem. 2013 Apr 11; 56(7):3024-32. PMID: 23451760; PMCID: PMC3632207.
    68. Li H, Jamal J, Chreifi G, Venkatesh V, Abou-Ziab H, Poulos TL. Dissecting the kinetics of the NADP(+)-FADH2 charge transfer complex and flavin semiquinones in neuronal nitric oxide synthase. J Inorg Biochem. 2013 Jul; 124:1-10. PMID: 23567464; PMCID: PMC3716911.
    69. Li H, Li C, Zhou S, Poulos TL, Gershon PD. Domain-level rocking motion within a polymerase that translocates on single-stranded nucleic acid. Acta Crystallogr D Biol Crystallogr. 2013 Apr; 69(Pt 4):617-24. PMID: 23519670; PMCID: PMC3606039.
    70. Yang Z, Misner B, Ji H, Poulos TL, Silverman RB, Meyskens FL, Yang S. Targeting nitric oxide signaling with nNOS inhibitors as a novel strategy for the therapy and prevention of human melanoma. Antioxid Redox Signal. 2013 Aug 10; 19(5):433-47. PMID: 23199242; PMCID: PMC3704054.
    71. Poulos TL, Madrona Y. Oxygen activation and redox partner binding in cytochromes P450. Biotechnol Appl Biochem. 2013 Jan-Feb; 60(1):128-33. PMID: 23587000.
      View in: PubMed
    72. Li H, Xue F, Kraus JM, Ji H, Labby KJ, Mataka J, Delker SL, Martásek P, Roman LJ, Poulos TL, Silverman RB. Cyclopropyl- and methyl-containing inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2013 Mar 01; 21(5):1333-43. PMID: 23352768; PMCID: PMC3574199.
    73. Salerno JC, Ray K, Poulos T, Li H, Ghosh DK. Calmodulin activates neuronal nitric oxide synthase by enabling transitions between conformational states. FEBS Lett. 2013 Jan 04; 587(1):44-7. PMID: 23159936.
      View in: PubMed
    74. Jasion VS, Doukov T, Pineda SH, Li H, Poulos TL. Crystal structure of the Leishmania major peroxidase-cytochrome c complex. Proc Natl Acad Sci U S A. 2012 Nov 06; 109(45):18390-4. PMID: 23100535; PMCID: PMC3494967.
    75. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Nat Biotechnol. 2012 Nov; 30(11):1143-8. PMID: 23086203; PMCID: PMC3699407.
    76. Poulos TL, Li H. Structural basis for isoform-selective inhibition in nitric oxide synthase. Acc Chem Res. 2013 Feb 19; 46(2):390-8. PMID: 23030042; PMCID: PMC3835173.
    77. Jensen LM, Meharenna YT, Davidson VL, Poulos TL, Hedman B, Wilmot CM, Sarangi R. Geometric and electronic structures of the His-Fe(IV)=O and His-Fe(IV)-Tyr hemes of MauG. J Biol Inorg Chem. 2012 Dec; 17(8):1241-55. PMID: 23053529; PMCID: PMC3508316.
    78. Sevrioukova IF, Poulos TL. Understanding the mechanism of cytochrome P450 3A4: recent advances and remaining problems. Dalton Trans. 2013 Mar 07; 42(9):3116-26. PMID: 23018626; PMCID: PMC3787833.
    79. Madrona Y, Tripathi S, Li H, Poulos TL. Crystal structures of substrate-free and nitrosyl cytochrome P450cin: implications for O(2) activation. Biochemistry. 2012 Aug 21; 51(33):6623-31. PMID: 22775403; PMCID: PMC3823376.
    80. Huang H, Ji H, Li H, Jing Q, Labby KJ, Martásek P, Roman LJ, Poulos TL, Silverman RB. Selective monocationic inhibitors of neuronal nitric oxide synthase. Binding mode insights from molecular dynamics simulations. J Am Chem Soc. 2012 Jul 18; 134(28):11559-72. PMID: 22731813; PMCID: PMC3399929.
    81. Benabbas A, Karunakaran V, Youn H, Poulos TL, Champion PM. Effect of DNA binding on geminate CO recombination kinetics in CO-sensing transcription factor CooA. J Biol Chem. 2012 Jun 22; 287(26):21729-40. PMID: 22544803; PMCID: PMC3381135.
    82. Jasion VS, Poulos TL. Leishmania major peroxidase is a cytochrome c peroxidase. Biochemistry. 2012 Mar 27; 51(12):2453-60. PMID: 22372542; PMCID: PMC3799847.
    83. Sevrioukova IF, Poulos TL. Interaction of human cytochrome P4503A4 with ritonavir analogs. Arch Biochem Biophys. 2012 Apr 15; 520(2):108-16. PMID: 22410611; PMCID: PMC3606017.
    84. Labby KJ, Xue F, Kraus JM, Ji H, Mataka J, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Intramolecular hydrogen bonding: a potential strategy for more bioavailable inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2012 Apr 01; 20(7):2435-43. PMID: 22370337; PMCID: PMC3303928.
    85. Sevrioukova IF, Poulos TL. Structural and mechanistic insights into the interaction of cytochrome P4503A4 with bromoergocryptine, a type I ligand. J Biol Chem. 2012 Jan 27; 287(5):3510-7. PMID: 22157006; PMCID: PMC3271004.
    86. Xue F, Kraus JM, Labby KJ, Ji H, Mataka J, Xia G, Li H, Delker SL, Roman LJ, Martásek P, Poulos TL, Silverman RB. Improved synthesis of chiral pyrrolidine inhibitors and their binding properties to neuronal nitric oxide synthase. J Med Chem. 2011 Sep 22; 54(18):6399-403. PMID: 21809851; PMCID: PMC3174355.
    87. Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL. Crystal structure of Leishmania major peroxidase and characterization of the compound i tryptophan radical. J Biol Chem. 2011 Jul 15; 286(28):24608-15. PMID: 21566139; PMCID: PMC3137036.
    88. Doukov T, Li H, Sharma A, Martell JD, Soltis SM, Silverman RB, Poulos TL. Temperature-dependent spin crossover in neuronal nitric oxide synthase bound with the heme-coordinating thioether inhibitors. J Am Chem Soc. 2011 Jun 01; 133(21):8326-34. PMID: 21534614; PMCID: PMC3193996.
    89. Xue F, Fang J, Delker SL, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Symmetric double-headed aminopyridines, a novel strategy for potent and membrane-permeable inhibitors of neuronal nitric oxide synthase. J Med Chem. 2011 Apr 14; 54(7):2039-48. PMID: 21410186; PMCID: PMC3072459.
    90. Delker SL, Xue F, Li H, Jamal J, Silverman RB, Poulos TL. Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase . Biochemistry. 2010 Dec 28; 49(51):10803-10. PMID: 21138269; PMCID: PMC3193998.
    91. Ji H, Delker SL, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Exploration of the active site of neuronal nitric oxide synthase by the design and synthesis of pyrrolidinomethyl 2-aminopyridine derivatives. J Med Chem. 2010 Nov 11; 53(21):7804-24. PMID: 20958055; PMCID: PMC2978073.
    92. Xue F, Li H, Delker SL, Fang J, Martásek P, Roman LJ, Poulos TL, Silverman RB. Potent, highly selective, and orally bioavailable gem-difluorinated monocationic inhibitors of neuronal nitric oxide synthase. J Am Chem Soc. 2010 Oct 13; 132(40):14229-38. PMID: 20843082; PMCID: PMC2956604.
    93. Sevrioukova IF, Poulos TL. Structure and mechanism of the complex between cytochrome P4503A4 and ritonavir. Proc Natl Acad Sci U S A. 2010 Oct 26; 107(43):18422-7. PMID: 20937904; PMCID: PMC2973003.
    94. Sevrioukova IF, Poulos TL. Structural biology of redox partner interactions in P450cam monooxygenase: a fresh look at an old system. Arch Biochem Biophys. 2011 Mar 01; 507(1):66-74. PMID: 20816746; PMCID: PMC3193999.
    95. Xue F, Li H, Fang J, Roman LJ, Martásek P, Poulos TL, Silverman RB. Peripheral but crucial: a hydrophobic pocket (Tyr(706), Leu(337), and Met(336)) for potent and selective inhibition of neuronal nitric oxide synthase. Bioorg Med Chem Lett. 2010 Nov 01; 20(21):6258-61. PMID: 20833542; PMCID: PMC2952696.
    96. Meharenna YT, Poulos TL. Using molecular dynamics to probe the structural basis for enhanced stability in thermal stable cytochromes P450. Biochemistry. 2010 Aug 10; 49(31):6680-6. PMID: 20593793; PMCID: PMC3193997.
    97. Xue F, Huang J, Ji H, Fang J, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Structure-based design, synthesis, and biological evaluation of lipophilic-tailed monocationic inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2010 Sep 01; 18(17):6526-37. PMID: 20673724; PMCID: PMC2925225.
    98. Sevrioukova IF, Poulos TL. Arginines 65 and 310 in putidaredoxin reductase are critical for interaction with putidaredoxin. Biochemistry. 2010 Jun 29; 49(25):5160-6. PMID: 20524621.
      View in: PubMed
    99. Delker SL, Ji H, Li H, Jamal J, Fang J, Xue F, Silverman RB, Poulos TL. Unexpected binding modes of nitric oxide synthase inhibitors effective in the prevention of a cerebral palsy phenotype in an animal model. J Am Chem Soc. 2010 Apr 21; 132(15):5437-42. PMID: 20337441; PMCID: PMC3202968.
    100. Meharenna YT, Doukov T, Li H, Soltis SM, Poulos TL. Crystallographic and single-crystal spectral analysis of the peroxidase ferryl intermediate. Biochemistry. 2010 Apr 13; 49(14):2984-6. PMID: 20230048; PMCID: PMC3202969.
    101. Sundaramoorthy M, Gold MH, Poulos TL. Ultrahigh (0.93A) resolution structure of manganese peroxidase from Phanerochaete chrysosporium: implications for the catalytic mechanism. J Inorg Biochem. 2010 Jun; 104(6):683-90. PMID: 20356630; PMCID: PMC2866031.
    102. Poulos TL. Thirty years of heme peroxidase structural biology. Arch Biochem Biophys. 2010 Aug 01; 500(1):3-12. PMID: 20206121; PMCID: PMC3202974.
    103. Sevrioukova IF, Poulos TL, Churbanova IY. Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex. J Biol Chem. 2010 Apr 30; 285(18):13616-20. PMID: 20179327; PMCID: PMC2859523.
    104. Martell JD, Li H, Doukov T, Martásek P, Roman LJ, Soltis M, Poulos TL, Silverman RB. Heme-coordinating inhibitors of neuronal nitric oxide synthase. Iron-thioether coordination is stabilized by hydrophobic contacts without increased inhibitor potency. J Am Chem Soc. 2010 Jan 20; 132(2):798-806. PMID: 20014790; PMCID: PMC2826131.
    105. Churbanova IY, Poulos TL, Sevrioukova IF. Production and characterization of a functional putidaredoxin reductase-putidaredoxin covalent complex. Biochemistry. 2010 Jan 12; 49(1):58-67. PMID: 19954240.
      View in: PubMed
    106. Doukov T, Li H, Soltis M, Poulos TL. Single crystal structural and absorption spectral characterizations of nitric oxide synthase complexed with N(omega)-hydroxy-L-arginine and diatomic ligands. Biochemistry. 2009 Nov 03; 48(43):10246-54. PMID: 19791770; PMCID: PMC3740733.
    107. Ioanoviciu A, Meharenna YT, Poulos TL, Ortiz de Montellano PR. DevS oxy complex stability identifies this heme protein as a gas sensor in Mycobacterium tuberculosis dormancy. Biochemistry. 2009 Jun 30; 48(25):5839-48. PMID: 19463006; PMCID: PMC2756985.
    108. Kumar MR, Pervitsky D, Chen L, Poulos T, Kundu S, Hargrove MS, Rivera EJ, Diaz A, Colón JL, Farmer PJ. Nitrosyl hydride (HNO) as an O2 analogue: long-lived HNO adducts of ferrous globins. Biochemistry. 2009 Jun 09; 48(22):5018-25. PMID: 19368336; PMCID: PMC2755260.
    109. Li C, Li H, Zhou S, Sun E, Yoshizawa J, Poulos TL, Gershon PD. Polymerase translocation with respect to single-stranded nucleic acid: looping or wrapping of primer around a poly(A) polymerase. Structure. 2009 May 13; 17(5):680-9. PMID: 19446524.
      View in: PubMed
    110. Igarashi J, Li H, Jamal J, Ji H, Fang J, Lawton GR, Silverman RB, Poulos TL. Crystal structures of constitutive nitric oxide synthases in complex with de novo designed inhibitors. J Med Chem. 2009 Apr 09; 52(7):2060-6. PMID: 19296678; PMCID: PMC3103786.
    111. Ji H, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Discovery of highly potent and selective inhibitors of neuronal nitric oxide synthase by fragment hopping. J Med Chem. 2009 Feb 12; 52(3):779-97. PMID: 19125620; PMCID: PMC2664101.
    112. Ji H, Tan S, Igarashi J, Li H, Derrick M, Martásek P, Roman LJ, Vásquez-Vivar J, Poulos TL, Silverman RB. Selective neuronal nitric oxide synthase inhibitors and the prevention of cerebral palsy. Ann Neurol. 2009 Feb; 65(2):209-17. PMID: 19235180; PMCID: PMC2658647.
    113. Li H, Das A, Sibhatu H, Jamal J, Sligar SG, Poulos TL. Exploring the electron transfer properties of neuronal nitric-oxide synthase by reversal of the FMN redox potential. J Biol Chem. 2008 Dec 12; 283(50):34762-72. PMID: 18852262; PMCID: PMC2596388.
    114. Meharenna YT, Oertel P, Bhaskar B, Poulos TL. Engineering ascorbate peroxidase activity into cytochrome c peroxidase. Biochemistry. 2008 Sep 30; 47(39):10324-32. PMID: 18771292; PMCID: PMC2770236.
    115. Fasan R, Meharenna YT, Snow CD, Poulos TL, Arnold FH. Evolutionary history of a specialized p450 propane monooxygenase. J Mol Biol. 2008 Nov 28; 383(5):1069-80. PMID: 18619466; PMCID: PMC2637765.
    116. Ji H, Stanton BZ, Igarashi J, Li H, Martásek P, Roman LJ, Poulos TL, Silverman RB. Minimal pharmacophoric elements and fragment hopping, an approach directed at molecular diversity and isozyme selectivity. Design of selective neuronal nitric oxide synthase inhibitors. J Am Chem Soc. 2008 Mar 26; 130(12):3900-14. PMID: 18321097; PMCID: PMC2929563.
    117. Meharenna YT, Slessor KE, Cavaignac SM, Poulos TL, De Voss JJ. The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin. J Biol Chem. 2008 Apr 18; 283(16):10804-12. PMID: 18270198; PMCID: PMC2447660.
    118. Ho WW, Li H, Nishida CR, de Montellano PR, Poulos TL. Crystal structure and properties of CYP231A2 from the thermoacidophilic archaeon Picrophilus torridus. Biochemistry. 2008 Feb 19; 47(7):2071-9. PMID: 18197710.
      View in: PubMed
    119. Ho WW, Li H, Eakanunkul S, Tong Y, Wilks A, Guo M, Poulos TL. Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins. J Biol Chem. 2007 Dec 07; 282(49):35796-802. PMID: 17925389.
      View in: PubMed
    120. Corbett MC, Latimer MJ, Poulos TL, Sevrioukova IF, Hodgson KO, Hedman B. Photoreduction of the active site of the metalloprotein putidaredoxin by synchrotron radiation. Acta Crystallogr D Biol Crystallogr. 2007 Sep; 63(Pt 9):951-60. PMID: 17704563.
      View in: PubMed
    121. Ibrahim M, Kuchinskas M, Youn H, Kerby RL, Roberts GP, Poulos TL, Spiro TG. Mechanism of the CO-sensing heme protein CooA: new insights from the truncated heme domain and UVRR spectroscopy. J Inorg Biochem. 2007 Nov; 101(11-12):1776-85. PMID: 17720248; PMCID: PMC2096632.
    122. Kimmich N, Das A, Sevrioukova I, Meharenna Y, Sligar SG, Poulos TL. Electron transfer between cytochrome P450cin and its FMN-containing redox partner, cindoxin. J Biol Chem. 2007 Sep 14; 282(37):27006-11. PMID: 17606612.
      View in: PubMed
    123. Levin AM, Murase K, Jackson PJ, Flinspach ML, Poulos TL, Weiss GA. Double barrel shotgun scanning of the caveolin-1 scaffolding domain. ACS Chem Biol. 2007 Jul 20; 2(7):493-500. PMID: 17602618; PMCID: PMC2532506.
    124. Seo J, Igarashi J, Li H, Martasek P, Roman LJ, Poulos TL, Silverman RB. Structure-based design and synthesis of N(omega)-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water. J Med Chem. 2007 May 03; 50(9):2089-99. PMID: 17425297; PMCID: PMC2562355.
    125. Poulos TL. The Janus nature of heme. Nat Prod Rep. 2007 Jun; 24(3):504-10. PMID: 17534526.
      View in: PubMed
    126. Borjigin M, Li H, Lanz ND, Kerby RL, Roberts GP, Poulos TL. Structure-based hypothesis on the activation of the CO-sensing transcription factor CooA. Acta Crystallogr D Biol Crystallogr. 2007 Mar; 63(Pt 3):282-7. PMID: 17327664.
      View in: PubMed
    127. Poulos TL. Structural biology of p450-oxy complexes. Drug Metab Rev. 2007; 39(2-3):557-66. PMID: 17786638.
      View in: PubMed
    128. Friedman J, Meharenna YT, Wilks A, Poulos TL. Diatomic ligand discrimination by the heme oxygenases from Neisseria meningitidis and Pseudomonas aeruginosa. J Biol Chem. 2007 Jan 12; 282(2):1066-71. PMID: 17095508.
      View in: PubMed
    129. Kuznetsov VY, Poulos TL, Sevrioukova IF. Putidaredoxin-to-cytochrome P450cam electron transfer: differences between the two reductive steps required for catalysis. Biochemistry. 2006 Oct 03; 45(39):11934-44. PMID: 17002293; PMCID: PMC2533851.
    130. Poulos TL. Soluble guanylate cyclase. Curr Opin Struct Biol. 2006 Dec; 16(6):736-43. PMID: 17015012.
      View in: PubMed
    131. Li H, Igarashi J, Jamal J, Yang W, Poulos TL. Structural studies of constitutive nitric oxide synthases with diatomic ligands bound. J Biol Inorg Chem. 2006 Sep; 11(6):753-68. PMID: 16804678.
      View in: PubMed
    132. Kuchinskas M, Li H, Conrad M, Roberts G, Poulos TL. The role of the DNA-binding domains in CooA activation. Biochemistry. 2006 Jun 13; 45(23):7148-53. PMID: 16752905.
      View in: PubMed
    133. Swartz L, Kuchinskas M, Li H, Poulos TL, Lanzilotta WN. Redox-dependent structural changes in the Azotobacter vinelandii bacterioferritin: new insights into the ferroxidase and iron transport mechanism. Biochemistry. 2006 Apr 11; 45(14):4421-8. PMID: 16584178.
      View in: PubMed
    134. Mbadugha BN, Seo J, Ji H, Martásek P, Roman LJ, Shea TM, Li H, Poulos TL, Silverman RB. Hydroxyl-terminated peptidomimetic inhibitors of neuronal nitric oxide synthase. Bioorg Med Chem. 2006 Jun 01; 14(11):3681-90. PMID: 16480878.
      View in: PubMed
    135. Li H, Flinspach ML, Igarashi J, Jamal J, Yang W, Gómez-Vidal JA, Litzinger EA, Huang H, Erdal EP, Silverman RB, Poulos TL. Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases. Biochemistry. 2005 Nov 22; 44(46):15222-9. PMID: 16285725.
      View in: PubMed
    136. Barrows TP, Poulos TL. Role of electrostatics and salt bridges in stabilizing the compound I radical in ascorbate peroxidase. Biochemistry. 2005 Nov 01; 44(43):14062-8. PMID: 16245922.
      View in: PubMed
    137. Poulos TL. Structural biology of heme monooxygenases. Biochem Biophys Res Commun. 2005 Dec 09; 338(1):337-45. PMID: 16185651.
      View in: PubMed
    138. Nagano S, Poulos TL. Crystallographic study on the dioxygen complex of wild-type and mutant cytochrome P450cam. Implications for the dioxygen activation mechanism. J Biol Chem. 2005 Sep 09; 280(36):31659-63. PMID: 15994329.
      View in: PubMed
    139. Bhaskar B, Poulos TL. The 1.13-A structure of iron-free cytochrome c peroxidase. J Biol Inorg Chem. 2005 Jun; 10(4):425-30. PMID: 15900441.
      View in: PubMed
    140. Sundaramoorthy M, Youngs HL, Gold MH, Poulos TL. High-resolution crystal structure of manganese peroxidase: substrate and inhibitor complexes. Biochemistry. 2005 May 03; 44(17):6463-70. PMID: 15850380.
      View in: PubMed
    141. Poulos TL. Intermediates in P450 catalysis. Philos Trans A Math Phys Eng Sci. 2005 Apr 15; 363(1829):793-806; discussion 1035-40. PMID: 15901536.
      View in: PubMed
    142. Nagano S, Cupp-Vickery JR, Poulos TL. Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF. J Biol Chem. 2005 Jun 10; 280(23):22102-7. PMID: 15824115.
      View in: PubMed
    143. Kuznetsov VY, Blair E, Farmer PJ, Poulos TL, Pifferitti A, Sevrioukova IF. The putidaredoxin reductase-putidaredoxin electron transfer complex: theoretical and experimental studies. J Biol Chem. 2005 Apr 22; 280(16):16135-42. PMID: 15716266.
      View in: PubMed
    144. Lad L, Koshkin A, de Montellano PR, Poulos TL. Crystal structures of the G139A, G139A-NO and G143H mutants of human heme oxygenase-1. A finely tuned hydrogen-bonding network controls oxygenase versus peroxidase activity. J Biol Inorg Chem. 2005 Mar; 10(2):138-46. PMID: 15690204.
      View in: PubMed
    145. Li H, Poulos TL. Structure-function studies on nitric oxide synthases. J Inorg Biochem. 2005 Jan; 99(1):293-305. PMID: 15598508.
      View in: PubMed
    146. Wang J, Lad L, Poulos TL, Ortiz de Montellano PR. Regiospecificity determinants of human heme oxygenase: differential NADPH- and ascorbate-dependent heme cleavage by the R183E mutant. J Biol Chem. 2005 Jan 28; 280(4):2797-806. PMID: 15525643.
      View in: PubMed
    147. Lad L, Ortiz de Montellano PR, Poulos TL. Crystal structures of ferrous and ferrous-NO forms of verdoheme in a complex with human heme oxygenase-1: catalytic implications for heme cleavage. J Inorg Biochem. 2004 Nov; 98(11):1686-95. PMID: 15522396.
      View in: PubMed
    148. Poulos TL. Structural and functional diversity in heme monooxygenases. Drug Metab Dispos. 2005 Jan; 33(1):10-8. PMID: 15475411.
      View in: PubMed
    149. Wang J, Niemevz F, Lad L, Huang L, Alvarez DE, Buldain G, Poulos TL, de Montellano PR. Human heme oxygenase oxidation of 5- and 15-phenylhemes. J Biol Chem. 2004 Oct 08; 279(41):42593-604. PMID: 15297453.
      View in: PubMed
    150. Meharenna YT, Li H, Hawkes DB, Pearson AG, De Voss J, Poulos TL. Crystal structure of P450cin in a complex with its substrate, 1,8-cineole, a close structural homologue to D-camphor, the substrate for P450cam. Biochemistry. 2004 Jul 27; 43(29):9487-94. PMID: 15260491.
      View in: PubMed
    151. Nagano S, Tosha T, Ishimori K, Morishima I, Poulos TL. Crystal structure of the cytochrome p450cam mutant that exhibits the same spectral perturbations induced by putidaredoxin binding. J Biol Chem. 2004 Oct 08; 279(41):42844-9. PMID: 15269210.
      View in: PubMed
    152. Barrows TP, Bhaskar B, Poulos TL. Electrostatic control of the tryptophan radical in cytochrome c peroxidase. Biochemistry. 2004 Jul 13; 43(27):8826-34. PMID: 15236591.
      View in: PubMed
    153. Flinspach M, Li H, Jamal J, Yang W, Huang H, Silverman RB, Poulos TL. Structures of the neuronal and endothelial nitric oxide synthase heme domain with D-nitroarginine-containing dipeptide inhibitors bound. Biochemistry. 2004 May 11; 43(18):5181-7. PMID: 15122883.
      View in: PubMed
    154. Friedman J, Lad L, Li H, Wilks A, Poulos TL. Structural basis for novel delta-regioselective heme oxygenation in the opportunistic pathogen Pseudomonas aeruginosa. Biochemistry. 2004 May 11; 43(18):5239-45. PMID: 15122889.
      View in: PubMed
    155. Guo M, Bhaskar B, Li H, Barrows TP, Poulos TL. Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link. Proc Natl Acad Sci U S A. 2004 Apr 20; 101(16):5940-5. PMID: 15071191; PMCID: PMC395902.
    156. Lad L, Friedman J, Li H, Bhaskar B, Ortiz de Montellano PR, Poulos TL. Crystal structure of human heme oxygenase-1 in a complex with biliverdin. Biochemistry. 2004 Apr 06; 43(13):3793-801. PMID: 15049686.
      View in: PubMed
    157. Sevrioukova IF, Li H, Poulos TL. Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase. J Mol Biol. 2004 Feb 27; 336(4):889-902. PMID: 15095867.
      View in: PubMed
    158. Li H, Poulos TL. Crystallization of cytochromes P450 and substrate-enzyme interactions. Curr Top Med Chem. 2004; 4(16):1789-802. PMID: 15579108.
      View in: PubMed
    159. Flinspach ML, Li H, Jamal J, Yang W, Huang H, Hah JM, Gómez-Vidal JA, Litzinger EA, Silverman RB, Poulos TL. Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase. Nat Struct Mol Biol. 2004 Jan; 11(1):54-9. PMID: 14718923.
      View in: PubMed
    160. Ji H, Li H, Flinspach M, Poulos TL, Silverman RB. Computer modeling of selective regions in the active site of nitric oxide synthases: implication for the design of isoform-selective inhibitors. J Med Chem. 2003 Dec 18; 46(26):5700-11. PMID: 14667223.
      View in: PubMed
    161. Poulos TL. The past and present of P450cam structural biology. Biochem Biophys Res Commun. 2003 Dec 05; 312(1):35-9. PMID: 14630013.
      View in: PubMed
    162. Poulos TL. Cytochrome P450 flexibility. Proc Natl Acad Sci U S A. 2003 Nov 11; 100(23):13121-2. PMID: 14597705; PMCID: PMC263725.
    163. Sevrioukova IF, Garcia C, Li H, Bhaskar B, Poulos TL. Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida. J Mol Biol. 2003 Oct 17; 333(2):377-92. PMID: 14529624.
      View in: PubMed
    164. Bretscher LE, Li H, Poulos TL, Griffith OW. Structural characterization and kinetics of nitric-oxide synthase inhibition by novel N5-(iminoalkyl)- and N5-(iminoalkenyl)-ornithines. J Biol Chem. 2003 Nov 21; 278(47):46789-97. PMID: 12960153.
      View in: PubMed
    165. Nagano S, Li H, Shimizu H, Nishida C, Ogura H, Ortiz de Montellano PR, Poulos TL. Crystal structures of epothilone D-bound, epothilone B-bound, and substrate-free forms of cytochrome P450epoK. J Biol Chem. 2003 Nov 07; 278(45):44886-93. PMID: 12933799.
      View in: PubMed
    166. Yano JK, Poulos TL. New understandings of thermostable and peizostable enzymes. Curr Opin Biotechnol. 2003 Aug; 14(4):360-5. PMID: 12943843.
      View in: PubMed
    167. Lad L, Wang J, Li H, Friedman J, Bhaskar B, Ortiz de Montellano PR, Poulos TL. Crystal structures of the ferric, ferrous, and ferrous-NO forms of the Asp140Ala mutant of human heme oxygenase-1: catalytic implications. J Mol Biol. 2003 Jul 11; 330(3):527-38. PMID: 12842469.
      View in: PubMed
    168. Friedman J, Lad L, Deshmukh R, Li H, Wilks A, Poulos TL. Crystal structures of the NO- and CO-bound heme oxygenase from Neisseriae meningitidis. Implications for O2 activation. J Biol Chem. 2003 Sep 05; 278(36):34654-9. PMID: 12819228.
      View in: PubMed
    169. Jia Q, Cai T, Huang M, Li H, Xian M, Poulos TL, Wang PG. Isoform-selective substrates of nitric oxide synthase. J Med Chem. 2003 Jun 05; 46(12):2271-4. PMID: 12773030.
      View in: PubMed
    170. Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL. High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase. Biochemistry. 2003 May 20; 42(19):5600-8. PMID: 12741816.
      View in: PubMed
    171. Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL. A novel heme and peroxide-dependent tryptophan-tyrosine cross-link in a mutant of cytochrome c peroxidase. J Mol Biol. 2003 Apr 18; 328(1):157-66. PMID: 12684005.
      View in: PubMed
    172. Lad L, Schuller DJ, Shimizu H, Friedman J, Li H, Ortiz de Montellano PR, Poulos TL. Comparison of the heme-free and -bound crystal structures of human heme oxygenase-1. J Biol Chem. 2003 Mar 07; 278(10):7834-43. PMID: 12500973.
      View in: PubMed
    173. Li H, Shimizu H, Flinspach M, Jamal J, Yang W, Xian M, Cai T, Wen EZ, Jia Q, Wang PG, Poulos TL. The novel binding mode of N-alkyl-N'-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO biosynthesis. Biochemistry. 2002 Nov 26; 41(47):13868-75. PMID: 12437343.
      View in: PubMed
    174. Yano JK, Blasco F, Li H, Schmid RD, Henne A, Poulos TL. Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus. J Biol Chem. 2003 Jan 03; 278(1):608-16. PMID: 12401810.
      View in: PubMed
    175. Sevrioukova IF, Poulos TL. Putidaredoxin reductase, a new function for an old protein. J Biol Chem. 2002 Jul 12; 277(28):25831-9. PMID: 12011076.
      View in: PubMed
    176. Bhaskar B, Bonagura CA, Li H, Poulos TL. Cation-induced stabilization of the engineered cation-binding loop in cytochrome c peroxidase (CcP). Biochemistry. 2002 Feb 26; 41(8):2684-93. PMID: 11851415.
      View in: PubMed
    177. Li H, Yano JK, Poulos TL. Molecular replacement in P450 crystal structure determinations. Methods Enzymol. 2002; 357:79-93. PMID: 12424900.
      View in: PubMed
    178. Poulos TL, Raman CS, Li H. NO news is good news. Structure. 1998 Mar 15; 6(3):255-8. PMID: 9551547.
      View in: PubMed