Huang, Lan

InstitutionUniversity of California, Irvine
DepartmentPhysiology and Biophysics
Address1001 Health Sciences Road
CA 92697-3950
Phone(949) 824-8548
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    Collapse Research 
    Collapse Research Activities and Funding
    In Vivo Interactome and Dynamics of Cullin-Ring Ligases
    NIH/NIGMS R01GM106003Jul 1, 2013 - Jun 30, 2017
    Role: Principal Investigator
    Function and Regulation of the CSN in the NF-kB Activation Pathway
    NIH/NCI R21CA161807Jun 1, 2012 - May 31, 2014
    Role: Principal Investigator
    Purchase of LTQ-Orbitrap Hybrid Mass Spectrometer
    NIH/NCRR S10RR023552Mar 1, 2007 - Feb 28, 2008
    Role: Principal Investigator
    Proteomics of the Proteasome Interacting Networks
    NIH/NIGMS R01GM074830Aug 1, 2005 - Jul 31, 2019
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Gutierrez CB, Block SA, Yu C, Soohoo SM, Huszagh AS, Rychnovsky SD, Huang L. Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein-Protein Interactions. Anal Chem. 2018 Jun 05. PMID: 29792801.
      View in: PubMed
    2. Alkafeef SS, Yu C, Huang L, Liu H. Wor1 establishes opaque cell fate through inhibition of the general co-repressor Tup1 in Candida albicans. PLoS Genet. 2018 Jan; 14(1):e1007176. PMID: 29337983.
      View in: PubMed
    3. Yu C, Huang L. Cross-Linking Mass Spectrometry: An Emerging Technology for Interactomics and Structural Biology. Anal Chem. 2018 Jan 02; 90(1):144-165. PMID: 29160693.
      View in: PubMed
    4. Kim JK, Liu J, Hu X, Yu C, Roskamp K, Sankaran B, Huang L, Komives EA, Qiao F. Structural Basis for Shelterin Bridge Assembly. Mol Cell. 2017 Nov 16; 68(4):698-714.e5. PMID: 29149597.
      View in: PubMed
    5. Wang X, Chemmama IE, Yu C, Huszagh A, Xu Y, Viner R, Block SA, Cimermancic P, Rychnovsky SD, Ye Y, Sali A, Huang L. The proteasome-interacting Ecm29 protein disassembles the 26S proteasome in response to oxidative stress. J Biol Chem. 2017 09 29; 292(39):16310-16320. PMID: 28821611.
      View in: PubMed
    6. Han JW, Valdez JL, Ho DV, Lee CS, Kim HM, Wang X, Huang L, Chan JY. Nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) is regulated by O-GlcNAc transferase. Free Radic Biol Med. 2017 Sep; 110:196-205. PMID: 28625484.
      View in: PubMed
    7. Wang X, Cimermancic P, Yu C, Schweitzer A, Chopra N, Engel JL, Greenberg C, Huszagh AS, Beck F, Sakata E, Yang Y, Novitsky EJ, Leitner A, Nanni P, Kahraman A, Guo X, Dixon JE, Rychnovsky SD, Aebersold R, Baumeister W, Sali A, Huang L. Molecular Details Underlying Dynamic Structures and Regulation of the Human 26S Proteasome. Mol Cell Proteomics. 2017 May; 16(5):840-854. PMID: 28292943.
      View in: PubMed
    8. Yu C, Huszagh A, Viner R, Novitsky EJ, Rychnovsky SD, Huang L. Developing a Multiplexed Quantitative Cross-Linking Mass Spectrometry Platform for Comparative Structural Analysis of Protein Complexes. Anal Chem. 2016 Oct 18; 88(20):10301-10308. PMID: 27626298.
      View in: PubMed
    9. Gutierrez CB, Yu C, Novitsky EJ, Huszagh AS, Rychnovsky SD, Huang L. Developing an Acidic Residue Reactive and Sulfoxide-Containing MS-Cleavable Homobifunctional Cross-Linker for Probing Protein-Protein Interactions. Anal Chem. 2016 Aug 16; 88(16):8315-22. PMID: 27417384; PMCID: PMC4989248.
    10. Yu C, Yang Y, Wang X, Guan S, Fang L, Liu F, Walters KJ, Kaiser P, Huang L. Characterization of Dynamic UbR-Proteasome Subcomplexes by In vivo Cross-linking (X) Assisted Bimolecular Tandem Affinity Purification (XBAP) and Label-free Quantitation. Mol Cell Proteomics. 2016 07; 15(7):2279-92. PMID: 27114451; PMCID: PMC4937504 [Available on 07/01/17].
    11. Zhang HH, Lechuga TJ, Chen Y, Yang Y, Huang L, Chen DB. Quantitative Proteomics Analysis of VEGF-Responsive Endothelial Protein S-Nitrosylation Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and LC-MS/MS. Biol Reprod. 2016 05; 94(5):114. PMID: 27075618; PMCID: PMC4939742 [Available on 05/01/17].
    12. Yu C, Mao H, Novitsky EJ, Tang X, Rychnovsky SD, Zheng N, Huang L. Gln40 deamidation blocks structural reconfiguration and activation of SCF ubiquitin ligase complex by Nedd8. Nat Commun. 2015 Dec 03; 6:10053. PMID: 26632597; PMCID: PMC4686759.
    13. Kandur WV, Kao A, Vellucci D, Huang L, Rychnovsky SD. Design of CID-cleavable protein cross-linkers: identical mass modifications for simpler sequence analysis. Org Biomol Chem. 2015 Oct 14; 13(38):9793-807. PMID: 26269432; PMCID: PMC4581996.
    14. Jiang L, Kosenko A, Yu C, Huang L, Li X, Hoshi N. Activation of m1 muscarinic acetylcholine receptor induces surface transport of KCNQ channels through a CRMP-2-mediated pathway. J Cell Sci. 2015 Nov 15; 128(22):4235-45. PMID: 26446259; PMCID: PMC4712786 [Available on 11/15/16].
    15. Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K, Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S. Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. PLoS Genet. 2015; 11(9):e1005528. PMID: 26421679; PMCID: PMC4589538.
    16. Liu J, Yu C, Hu X, Kim JK, Bierma JC, Jun HI, Rychnovsky SD, Huang L, Qiao F. Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis. Cell Rep. 2015 Sep 29; 12(12):2169-80. PMID: 26365187; PMCID: PMC4591219.
    17. Burke AM, Kandur W, Novitsky EJ, Kaake RM, Yu C, Kao A, Vellucci D, Huang L, Rychnovsky SD. Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein-protein interactions by mass spectrometry. Org Biomol Chem. 2015 May 07; 13(17):5030-7. PMID: 25823605; PMCID: PMC4420144.
    18. Zhang HH, Wang W, Feng L, Yang Y, Zheng J, Huang L, Chen DB. S-nitrosylation of Cofilin-1 Serves as a Novel Pathway for VEGF-Stimulated Endothelial Cell Migration. J Cell Physiol. 2015 Feb; 230(2):406-17. PMID: 25056928.
      View in: PubMed
    19. Kaake RM, Wang X, Burke A, Yu C, Kandur W, Yang Y, Novtisky EJ, Second T, Duan J, Kao A, Guan S, Vellucci D, Rychnovsky SD, Huang L. A new in vivo cross-linking mass spectrometry platform to define protein-protein interactions in living cells. Mol Cell Proteomics. 2014 Dec; 13(12):3533-43. PMID: 25253489; PMCID: PMC4256503.
    20. Satohisa S, Zhang HH, Feng L, Yang YY, Huang L, Chen DB. Endogenous NO upon estradiol-17ß stimulation and NO donor differentially regulate mitochondrial S-nitrosylation in endothelial cells. Endocrinology. 2014 Aug; 155(8):3005-16. PMID: 24877627; PMCID: PMC4098011.
    21. Kaake RM, Kao A, Yu C, Huang L. Characterizing the dynamics of proteasome complexes by proteomics approaches. Antioxid Redox Signal. 2014 Dec 10; 21(17):2444-56. PMID: 24423446; PMCID: PMC4241863.
    22. Yu C, Kandur W, Kao A, Rychnovsky S, Huang L. Developing new isotope-coded mass spectrometry-cleavable cross-linkers for elucidating protein structures. Anal Chem. 2014 Feb 18; 86(4):2099-106. PMID: 24471733; PMCID: PMC3985771.
    23. Wang X, Huang L. Defining dynamic protein interactions using SILAC-based quantitative mass spectrometry. Methods Mol Biol. 2014; 1188:191-205. PMID: 25059613; PMCID: PMC4444047.
    24. Gordon EA, Whisenant TC, Zeller M, Kaake RM, Gordon WM, Krotee P, Patel V, Huang L, Baldi P, Bardwell L. Combining docking site and phosphosite predictions to find new substrates: identification of smoothelin-like-2 (SMTNL2) as a c-Jun N-terminal kinase (JNK) substrate. Cell Signal. 2013 Dec; 25(12):2518-29. PMID: 23981301; PMCID: PMC4132694.
    25. Anand P, Cermelli S, Li Z, Kassan A, Bosch M, Sigua R, Huang L, Ouellette AJ, Pol A, Welte MA, Gross SP. A novel role for lipid droplets in the organismal antibacterial response. Elife. 2012 Nov 13; 1:e00003. PMID: 23150794; PMCID: PMC3491588.
    26. Yen JL, Flick K, Papagiannis CV, Mathur R, Tyrrell A, Ouni I, Kaake RM, Huang L, Kaiser P. Signal-induced disassembly of the SCF ubiquitin ligase complex by Cdc48/p97. Mol Cell. 2012 Oct 26; 48(2):288-97. PMID: 23000173; PMCID: PMC3483439.
    27. Kao A, Randall A, Yang Y, Patel VR, Kandur W, Guan S, Rychnovsky SD, Baldi P, Huang L. Mapping the structural topology of the yeast 19S proteasomal regulatory particle using chemical cross-linking and probabilistic modeling. Mol Cell Proteomics. 2012 Dec; 11(12):1566-77. PMID: 22550050; PMCID: PMC3518129.
    28. Fang L, Kaake RM, Patel VR, Yang Y, Baldi P, Huang L. Mapping the protein interaction network of the human COP9 signalosome complex using a label-free QTAX strategy. Mol Cell Proteomics. 2012 May; 11(5):138-47. PMID: 22474085; PMCID: PMC3418852.
    29. Xu J, Reddy BJ, Anand P, Shu Z, Cermelli S, Mattson MK, Tripathy SK, Hoss MT, James NS, King SJ, Huang L, Bardwell L, Gross SP. Casein kinase 2 reverses tail-independent inactivation of kinesin-1. Nat Commun. 2012 Mar 27; 3:754. PMID: 22453827; PMCID: PMC3574636.
    30. Aiken CT, Kaake RM, Wang X, Huang L. Oxidative stress-mediated regulation of proteasome complexes. Mol Cell Proteomics. 2011 May; 10(5):R110.006924. PMID: 21543789; PMCID: PMC3098605.
    31. Furuta S, Jeng YM, Zhou L, Huang L, Kuhn I, Bissell MJ, Lee WH. IL-25 causes apoptosis of IL-25R-expressing breast cancer cells without toxicity to nonmalignant cells. Sci Transl Med. 2011 Apr 13; 3(78):78ra31. PMID: 21490275; PMCID: PMC3199022.
    32. Aphasizheva I, Maslov D, Wang X, Huang L, Aphasizhev R. Pentatricopeptide repeat proteins stimulate mRNA adenylation/uridylation to activate mitochondrial translation in trypanosomes. Mol Cell. 2011 Apr 08; 42(1):106-17. PMID: 21474072; PMCID: PMC3073060.
    33. Tsai BP, Wang X, Huang L, Waterman ML. Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Mol Cell Proteomics. 2011 Apr; 10(4):M110.007385. PMID: 21285413; PMCID: PMC3069349.
    34. Wang X, Yen J, Kaiser P, Huang L. Regulation of the 26S proteasome complex during oxidative stress. Sci Signal. 2010 Dec 07; 3(151):ra88. PMID: 21139140; PMCID: PMC3140957.
    35. Whisenant TC, Ho DT, Benz RW, Rogers JS, Kaake RM, Gordon EA, Huang L, Baldi P, Bardwell L. Computational prediction and experimental verification of new MAP kinase docking sites and substrates including Gli transcription factors. PLoS Comput Biol. 2010 Aug 26; 6(8). PMID: 20865152; PMCID: PMC2928751.
    36. Kao A, Chiu CL, Vellucci D, Yang Y, Patel VR, Guan S, Randall A, Baldi P, Rychnovsky SD, Huang L. Development of a novel cross-linking strategy for fast and accurate identification of cross-linked peptides of protein complexes. Mol Cell Proteomics. 2011 Jan; 10(1):M110.002212. PMID: 20736410; PMCID: PMC3013449.
    37. Kaake RM, Wang X, Huang L. Profiling of protein interaction networks of protein complexes using affinity purification and quantitative mass spectrometry. Mol Cell Proteomics. 2010 Aug; 9(8):1650-65. PMID: 20445003; PMCID: PMC2938061.
    38. Vellucci D, Kao A, Kaake RM, Rychnovsky SD, Huang L. Selective enrichment and identification of azide-tagged cross-linked peptides using chemical ligation and mass spectrometry. J Am Soc Mass Spectrom. 2010 Aug; 21(8):1432-45. PMID: 20472459; PMCID: PMC3119349.
    39. Kaake RM, Milenkovic T, Przulj N, Kaiser P, Huang L. Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy. J Proteome Res. 2010 Apr 05; 9(4):2016-29. PMID: 20170199; PMCID: PMC3140958.
    40. Ringpis GE, Aphasizheva I, Wang X, Huang L, Lathrop RH, Hatfield GW, Aphasizhev R. Mechanism of U insertion RNA editing in trypanosome mitochondria: the bimodal TUTase activity of the core complex. J Mol Biol. 2010 Jun 25; 399(5):680-95. PMID: 20362585; PMCID: PMC2885523.
    41. Thompson LM, Aiken CT, Kaltenbach LS, Agrawal N, Illes K, Khoshnan A, Martinez-Vincente M, Arrasate M, O'Rourke JG, Khashwji H, Lukacsovich T, Zhu YZ, Lau AL, Massey A, Hayden MR, Zeitlin SO, Finkbeiner S, Green KN, LaFerla FM, Bates G, Huang L, Patterson PH, Lo DC, Cuervo AM, Marsh JL, Steffan JS. IKK phosphorylates Huntingtin and targets it for degradation by the proteasome and lysosome. J Cell Biol. 2009 Dec 28; 187(7):1083-99. PMID: 20026656; PMCID: PMC2806289.
    42. Aiken CT, Steffan JS, Guerrero CM, Khashwji H, Lukacsovich T, Simmons D, Purcell JM, Menhaji K, Zhu YZ, Green K, Laferla F, Huang L, Thompson LM, Marsh JL. Phosphorylation of threonine 3: implications for Huntingtin aggregation and neurotoxicity. J Biol Chem. 2009 Oct 23; 284(43):29427-36. PMID: 19710014; PMCID: PMC2785575.
    43. Kodani A, Kristensen I, Huang L, Sütterlin C. GM130-dependent control of Cdc42 activity at the Golgi regulates centrosome organization. Mol Biol Cell. 2009 Feb; 20(4):1192-200. PMID: 19109421; PMCID: PMC2642749.
    44. Weng J, Aphasizheva I, Etheridge RD, Huang L, Wang X, Falick AM, Aphasizhev R. Guide RNA-binding complex from mitochondria of trypanosomatids. Mol Cell. 2008 Oct 24; 32(2):198-209. PMID: 18951088; PMCID: PMC2645705.
    45. Fang L, Wang X, Yamoah K, Chen PL, Pan ZQ, Huang L. Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry. J Proteome Res. 2008 Nov; 7(11):4914-25. PMID: 18850735; PMCID: PMC2676908.
    46. Meierhofer D, Wang X, Huang L, Kaiser P. Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry. J Proteome Res. 2008 Oct; 7(10):4566-76. PMID: 18781797; PMCID: PMC2758155.
    47. Guerrero C, Milenkovic T, Przulj N, Kaiser P, Huang L. Characterization of the proteasome interaction network using a QTAX-based tag-team strategy and protein interaction network analysis. Proc Natl Acad Sci U S A. 2008 Sep 09; 105(36):13333-8. PMID: 18757749; PMCID: PMC2533190.
    48. Jones J, Wu K, Yang Y, Guerrero C, Nillegoda N, Pan ZQ, Huang L. A targeted proteomic analysis of the ubiquitin-like modifier nedd8 and associated proteins. J Proteome Res. 2008 Mar; 7(3):1274-87. PMID: 18247557; PMCID: PMC2676899.
    49. Kaiser P, Meierhofer D, Wang X, Huang L. Tandem affinity purification combined with mass spectrometry to identify components of protein complexes. Methods Mol Biol. 2008; 439:309-26. PMID: 18370112; PMCID: PMC3228642.
    50. Wang X, Huang L. Identifying dynamic interactors of protein complexes by quantitative mass spectrometry. Mol Cell Proteomics. 2008 Jan; 7(1):46-57. PMID: 17934176.
      View in: PubMed
    51. Wang X, Guerrero C, Kaiser P, Huang L. Proteomics of proteasome complexes and ubiquitinated proteins. Expert Rev Proteomics. 2007 Oct; 4(5):649-65. PMID: 17941820.
      View in: PubMed
    52. Wang X, Chen CF, Baker PR, Chen PL, Kaiser P, Huang L. Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. Biochemistry. 2007 Mar 20; 46(11):3553-65. PMID: 17323924.
      View in: PubMed
    53. Friedrich ML, Cui M, Hernandez JB, Weist BM, Andersen HM, Zhang X, Huang L, Walsh CM. Modulation of DRAK2 autophosphorylation by antigen receptor signaling in primary lymphocytes. J Biol Chem. 2007 Feb 16; 282(7):4573-84. PMID: 17182616.
      View in: PubMed
    54. Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, Auer B, Huang L, Kaiser P. HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Yeast. 2006 Jun; 23(8):623-32. PMID: 16823883.
      View in: PubMed
    55. Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P. A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Mol Cell Proteomics. 2006 Apr; 5(4):737-48. PMID: 16432255.
      View in: PubMed
    56. Guerrero C, Tagwerker C, Kaiser P, Huang L. An integrated mass spectrometry-based proteomic approach: quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network. Mol Cell Proteomics. 2006 Feb; 5(2):366-78. PMID: 16284124.
      View in: PubMed
    57. Kaiser P, Huang L. Global approaches to understanding ubiquitination. Genome Biol. 2005; 6(10):233. PMID: 16207362; PMCID: PMC1257457.
    58. Huang L, Burlingame AL. Comprehensive mass spectrometric analysis of the 20S proteasome complex. Methods Enzymol. 2005; 405:187-236. PMID: 16413316.
      View in: PubMed
    59. Chou S, Huang L, Liu H. Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeast. Cell. 2004 Dec 29; 119(7):981-90. PMID: 15620356.
      View in: PubMed